scholarly journals Genes involved in centrosome-independent mitotic spindle assembly in Drosophila S2 cells

2013 ◽  
Vol 110 (49) ◽  
pp. 19808-19813 ◽  
Author(s):  
S. Moutinho-Pereira ◽  
N. Stuurman ◽  
O. Afonso ◽  
M. Hornsveld ◽  
P. Aguiar ◽  
...  
Keyword(s):  
Mitotic Spindle ◽  
Spindle Assembly ◽  
S2 Cells ◽  
Drosophila S2 Cells ◽  
Mitotic Spindle Assembly

scholarly journals Kinetochore-driven formation of kinetochore fibers contributes to spindle assembly during animal mitosis

2004 ◽  
Vol 167 (5) ◽  
pp. 831-840 ◽  
Author(s):  
Helder Maiato ◽  
Conly L. Rieder ◽  
Alexey Khodjakov
Keyword(s):  
Mitotic Spindle ◽  
Spindle Assembly ◽  
Spindle Pole ◽  
S2 Cells ◽  
Spindle Poles ◽  
Drosophila S2 Cells ◽  
Mitotic Spindle Assembly ◽  
Normal Mitosis ◽  
Proper Orientation ◽  
Dependent Mechanism

It is now clear that a centrosome-independent pathway for mitotic spindle assembly exists even in cells that normally possess centrosomes. The question remains, however, whether this pathway only activates when centrosome activity is compromised, or whether it contributes to spindle morphogenesis during a normal mitosis. Here, we show that many of the kinetochore fibers (K-fibers) in centrosomal Drosophila S2 cells are formed by the kinetochores. Initially, kinetochore-formed K-fibers are not oriented toward a spindle pole but, as they grow, their minus ends are captured by astral microtubules (MTs) and transported poleward through a dynein-dependent mechanism. This poleward transport results in chromosome bi-orientation and congression. Furthermore, when individual K-fibers are severed by laser microsurgery, they regrow from the kinetochore outward via MT plus-end polymerization at the kinetochore. Thus, even in the presence of centrosomes, the formation of some K-fibers is initiated by the kinetochores. However, centrosomes facilitate the proper orientation of K-fibers toward spindle poles by integrating them into a common spindle.

scholarly journals Genes Required for Mitotic Spindle Assembly in Drosophila S2 Cells

Science ◽  
2007 ◽  
Vol 316 (5823) ◽  
pp. 417-421 ◽  
Author(s):  
G. Goshima ◽  
R. Wollman ◽  
S. S. Goodwin ◽  
N. Zhang ◽  
J. M. Scholey ◽  
...  
Keyword(s):  
Mitotic Spindle ◽  
Spindle Assembly ◽  
S2 Cells ◽  
Drosophila S2 Cells ◽  
Mitotic Spindle Assembly

scholarly journals Novel insights into the mechanisms of mitotic spindle assembly by NEK kinases

2016 ◽  
Vol 3 (3) ◽  
pp. e1062952 ◽  
Author(s):  
Suzanna L. Prosser ◽  
Laura O'Regan ◽  
Andrew M. Fry
Keyword(s):  
Mitotic Spindle ◽  
Spindle Assembly ◽  
Mitotic Spindle Assembly
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