scholarly journals Ecdysone-inducible gene expression in mammalian cells and transgenic mice.

1996 ◽  
Vol 93 (8) ◽  
pp. 3346-3351 ◽  
Author(s):  
D. No ◽  
T. P. Yao ◽  
R. M. Evans
Keyword(s):  
Gene Expression ◽  
Transgenic Mice ◽  
Mammalian Cells ◽  
Inducible Gene Expression ◽  
Expression In Mammalian Cells ◽  
Inducible Gene

Inducible gene expression in mammalian cells and transgenic mice

1997 ◽  
Vol 8 (5) ◽  
pp. 608-616 ◽  
Author(s):  
Enriquez Saez ◽  
David No ◽  
Andrew West ◽  
Ronald M Evans
Keyword(s):  
Gene Expression ◽  
Transgenic Mice ◽  
Mammalian Cells ◽  
Inducible Gene Expression ◽  
Expression In Mammalian Cells ◽  
Inducible Gene

Ecdysone as Regulator of Inducible Gene Expression in Mammalian Cells

2006 ◽  
Vol 2006 (1) ◽  
pp. pdb.prot3186 ◽  
Author(s):  
Joseph Sambrook ◽  
David W. Russell
Keyword(s):  
Gene Expression ◽  
Mammalian Cells ◽  
Inducible Gene Expression ◽  
Expression In Mammalian Cells ◽  
Inducible Gene

Behavior analysis of Zaki-4 transgenic mice, with the inducible gene expression in brain using tTA system

2009 ◽  
Vol 65 ◽  
pp. S129
Author(s):  
Keiko Toyama ◽  
Nobuyuki Yamasaki ◽  
Keizo Takao ◽  
Tsuyoshi Miyakawa
Keyword(s):  
Gene Expression ◽  
Transgenic Mice ◽  
Behavior Analysis ◽  
Inducible Gene Expression ◽  
Inducible Gene

Selecting the optimal Tet-On system for doxycycline-inducible gene expression in transiently transfected and stably transduced mammalian cells

2015 ◽  
Vol 11 (1) ◽  
pp. 71-79 ◽  
Author(s):  
Atze T. Das ◽  
Xue Zhou ◽  
Stefan W. Metz ◽  
Monique A. Vink ◽  
Ben Berkhout
Keyword(s):  
Gene Expression ◽  
Mammalian Cells ◽  
Inducible Gene Expression ◽  
Inducible Gene

scholarly journals Generation of H11-albumin-rtTA Transgenic Mice: A Tool for Inducible Gene Expression in the Liver

2018 ◽  
pp. g3.200963.2018
Author(s):  
Yu-Shan Li ◽  
Ran-Ran Meng ◽  
Xiu Chen ◽  
Cui-Ling Shang ◽  
Hong-Bin Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transgenic Mice ◽  
Inducible Gene Expression ◽  
Inducible Gene

Tetracycline-inducible gene expression in cultured rat renal CD cells and in intact CD from transgenic mice

2001 ◽  
Vol 281 (6) ◽  
pp. F1164-F1172 ◽  
Author(s):  
Stefania Puttini ◽  
Ahmed T. Beggah ◽  
Antoine Ouvrard-Pascaud ◽  
Christine Legris ◽  
Marcel Blot-Chabaud ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transgenic Mice ◽  
Cell Line ◽  
Reporter Gene ◽  
Hormone Receptors ◽  
Collecting Duct ◽  
Cortical Collecting Duct ◽  
Inducible Gene Expression ◽  
Inducible Gene

First published August 30, 2001; 10.1152/ajprenal.00360.2000.—The renal collecting duct (CD) plays a key role in the control of ion and fluid homeostasis. Several genetic diseases that involve mutations in genes encoding for ion transporters or hormone receptors specifically expressed in CD have been described. Suitable cellular or transgenic animal models expressing such mutated genes in an inducible manner should represent attractive systems for structure-function relationship analyses and the generation of appropriate physiopathological models of related diseases. Our first goal was to develop a CD cell line that allows inducible gene expression using the tetracycline-inducible system (Tet-On). We designed several strategies aimed at the development of a tight and highly inducible system in RCCD1 cells, a rat cortical collecting duct (CCD) cell line exhibiting several properties of the native CCD. Analysis of reporter gene expression demonstrated that the Tet-On system is suitable for inducible gene expression in these cells. In a second step, we have tested whether transgenic Tet-On mice expressing the tetracycline transactivator under the control of the human cytomegalovirus promoter were suitable for inducible gene expression in tubule epithelial cells. The results indicate that, in vivo , the inducible expression of the lacZ reporter gene appeared to be restricted to the CD. This particular strain of transgenic mice may therefore be useful for the expression of genes of interest in an inducible manner in the collecting duct.

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