scholarly journals A Molecular Handoff between Bacteriophage T7 DNA Primase and T7 DNA Polymerase Initiates DNA Synthesis

2004 ◽  
Vol 279 (29) ◽  
pp. 30554-30562 ◽  
Author(s):  
Masato Kato ◽  
Takuhiro Ito ◽  
Gerhard Wagner ◽  
Tom Ellenberger
Keyword(s):  
Dna Synthesis ◽  
Dna Polymerase ◽  
Bacteriophage T7 ◽  
Dna Primase

scholarly journals Primer release is the rate-limiting event in lagging-strand synthesis mediated by the T7 replisome

2016 ◽  
Vol 113 (21) ◽  
pp. 5916-5921 ◽  
Author(s):  
Alfredo J. Hernandez ◽  
Seung-Joo Lee ◽  
Charles C. Richardson
Keyword(s):  
Dna Synthesis ◽  
Dna Polymerase ◽  
Fragment Length ◽  
Dna Binding Protein ◽  
Okazaki Fragment ◽  
Dna Primase ◽  
Dna Strands ◽  
Leading Strand ◽  
Rate Limiting ◽  
Lagging Strand

DNA replication occurs semidiscontinuously due to the antiparallel DNA strands and polarity of enzymatic DNA synthesis. Although the leading strand is synthesized continuously, the lagging strand is synthesized in small segments designated Okazaki fragments. Lagging-strand synthesis is a complex event requiring repeated cycles of RNA primer synthesis, transfer to the lagging-strand polymerase, and extension effected by cooperation between DNA primase and the lagging-strand polymerase. We examined events controlling Okazaki fragment initiation using the bacteriophage T7 replication system. Primer utilization by T7 DNA polymerase is slower than primer formation. Slow primer release from DNA primase allows the polymerase to engage the complex and is followed by a slow primer handoff step. The T7 single-stranded DNA binding protein increases primer formation and extension efficiency but promotes limited rounds of primer extension. We present a model describing Okazaki fragment initiation, the regulation of fragment length, and their implications for coordinated leading- and lagging-strand DNA synthesis.

Mechanism of initiation of in vitro DNA synthesis by the immunopurified complex between yeast DNA polymerase I and DNA primase

1986 ◽  
Vol 161 (2) ◽  
pp. 435-440 ◽  
Author(s):  
Gianfranco BADARACCO ◽  
Paola VALSASNINI ◽  
Marco FOIANI ◽  
Roberta BENFANTE ◽  
Giovanna LUCCHINI ◽  
...  
Keyword(s):  
Dna Synthesis ◽  
Dna Polymerase ◽  
Dna Polymerase I ◽  
Dna Primase ◽  
Polymerase I

Initiation, elongation and pausing of in vitro DNA synthesis catalyzed by immunopurified yeast DNA primase: DNA polymerase complex.

1985 ◽  
Vol 4 (5) ◽  
pp. 1313-1317 ◽  
Author(s):  
G. Badaracco ◽  
M. Bianchi ◽  
P. Valsasnini ◽  
G. Magni ◽  
P. Plevani
Keyword(s):  
Dna Synthesis ◽  
Dna Polymerase ◽  
Polymerase Complex ◽  
Dna Primase

scholarly journals Yeast DNA primase and DNA polymerase activities. An analysis of RNA priming and its coupling to DNA synthesis.

1986 ◽  
Vol 261 (18) ◽  
pp. 8564-8569
Author(s):  
H Singh ◽  
R G Brooke ◽  
M H Pausch ◽  
G T Williams ◽  
C Trainor ◽  
...  
Keyword(s):  
Dna Synthesis ◽  
Dna Polymerase ◽  
Dna Primase
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