Faculty Opinions recommendation of Modular architecture of the bacteriophage T7 primase couples RNA primer synthesis to DNA synthesis.

2003 ◽  
Author(s):  
Edward Egelman
Keyword(s):  
Dna Synthesis ◽  
Bacteriophage T7 ◽  
Modular Architecture

scholarly journals Modular Architecture of the Bacteriophage T7 Primase Couples RNA Primer Synthesis to DNA Synthesis

2003 ◽  
Vol 11 (5) ◽  
pp. 1349-1360 ◽  
Author(s):  
Masato Kato ◽  
Takuhiro Ito ◽  
Gerhard Wagner ◽  
Charles C. Richardson ◽  
Tom Ellenberger
Keyword(s):  
Dna Synthesis ◽  
Bacteriophage T7 ◽  
Modular Architecture

Development of T7 phage and T7 phage containing apurinic sites in an exonuclease III, endonuclease IV double mutant of Escherichia coli

1992 ◽  
Vol 70 (7) ◽  
pp. 605-608 ◽  
Author(s):  
Giselle Sanchez ◽  
Margaret D. Mamet-Bratley
Keyword(s):  
Escherichia Coli ◽  
Dna Repair ◽  
Dna Synthesis ◽  
Double Mutant ◽  
Bacteriophage T7 ◽  
Bacterial Dna ◽  
Exonuclease Iii ◽  
Repair Enzymes ◽  
Phage Dna ◽  
T7 Phage

The development of bacteriophage T7 was examined in an Escherichia coli double mutant defective for the two major apurinic, apyrimidinic endonucleases (exonuclease III and endonuclease IV, xth nfo). In cells infected with phages containing apurinic sites, the defect in repair enzymes led to a decrease of phage survival and a total absence of bacterial DNA degradation and of phage DNA synthesis. These results directly demonstrate the toxic action of apurinic sites on bacteriophage T7 at the intracellular level and its alleviation by DNA repair. In addition, untreated T7 phage unexpectedly displayed reduced plating efficiency and decreased DNA synthesis in the xth nfo double mutant.Key words: apurinic sites, DNA repair, T7 phage.

Studies on bacteriophage T7 DNA synthesis in vitro

1975 ◽  
Vol 141 (3) ◽  
pp. 233-249 ◽  
Author(s):  
Eberhard Scherzinger ◽  
Günther Klotz
Keyword(s):  
Dna Synthesis ◽  
Bacteriophage T7

A novel mutant of bacteriophage T7 that is defective in early phage DNA synthesis

1980 ◽  
Vol 179 (3) ◽  
pp. 683-691 ◽  
Author(s):  
Richard North ◽  
Ian J. Molineux
Keyword(s):  
Dna Synthesis ◽  
Bacteriophage T7 ◽  
Phage Dna

scholarly journals A Molecular Handoff between Bacteriophage T7 DNA Primase and T7 DNA Polymerase Initiates DNA Synthesis

2004 ◽  
Vol 279 (29) ◽  
pp. 30554-30562 ◽  
Author(s):  
Masato Kato ◽  
Takuhiro Ito ◽  
Gerhard Wagner ◽  
Tom Ellenberger
Keyword(s):  
Dna Synthesis ◽  
Dna Polymerase ◽  
Bacteriophage T7 ◽  
Dna Primase

scholarly journals Visualization of Repair of Double-Strand Breaks in the Bacteriophage T7 Genome without Normal DNA Replication

2000 ◽  
Vol 182 (2) ◽  
pp. 327-336 ◽  
Author(s):  
Ying-Ta Lai ◽  
Warren Masker
Keyword(s):  
Dna Synthesis ◽  
High Efficiency ◽  
Strand Break ◽  
Double Strand Break ◽  
Double Strand Breaks ◽  
Bacteriophage T7 ◽  
Growth Step ◽  
Strand Breaks

ABSTRACT An in vitro system based on extracts of Escherichia coli infected with bacteriophage T7 is able to repair double-strand breaks in a T7 genome with efficiencies of 20% or more. To achieve this high repair efficiency it is necessary that the reaction mixtures contain molecules of donor DNA that bracket the double-strand break. Gaps as long as 1,600 nucleotides are repaired almost as efficiently as simple double-strand breaks. DNA synthesis was measured while repair was taking place. It was found that the amount of DNA synthesis associated with repair of a double-strand break was below the level of detection possible with this system. Furthermore, repair efficiencies were the same with or without normal levels of T7 DNA polymerase. However, the repair required the 5′→3′ exonuclease encoded by T7 gene 6. The high efficiency of DNA repair allowed visualization of the repaired product after in vitro repair, thereby assuring that the repair took place in vitro rather than during an in vivo growth step after packaging.

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