Rapid colorimetric determination of nitrate in plant tissue by nitration of salicylic acid

Total starch was previously extracted from plant tissue and enzymatically digested to D-Glucose. This protocol is based on the NZYtech GOD-POD kit's colorimetric determination of D-Glucose and includes basic calculations to convert D-Glucose back to total starch in the original tissue.

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Colorimetric Determination of Free Salicylic Acid in Aspirin and Urine by Scanometry as a New, Reliable, Inexpensive and Simple Method

Pharmaceutical Chemistry Journal ◽

10.1007/s11094-017-1607-2 ◽

2017 ◽

Vol 51 (4) ◽

pp. 324-329 ◽

Cited By ~ 1

Author(s):

Ardeshir Shokrollahi ◽

Zahra Mohammadpour ◽

Abdolkarim Abbaspour

Keyword(s):

Salicylic Acid ◽

Colorimetric Determination ◽

Simple Method

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COLORIMETRIC DETERMINATION OF CAROTENE IN PLANT TISSUE

PLANT PHYSIOLOGY ◽

10.1104/pp.10.2.325 ◽

1935 ◽

Vol 10 (2) ◽

pp. 325-340 ◽

Cited By ~ 17

Author(s):

Walter C. Russell ◽

M. W. Taylor ◽

D. F. Chichester

Keyword(s):

Plant Tissue ◽

Colorimetric Determination

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Colorimetric Determination of Copper with Pyridine and Salicylic Acid

Analytical Chemistry ◽

10.1021/ac60045a020 ◽

1950 ◽

Vol 22 (9) ◽

pp. 1166-1168 ◽

Cited By ~ 16

Author(s):

V. A. Gordieyeff

Keyword(s):

Salicylic Acid ◽

Colorimetric Determination ◽

Determination Of Copper

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Improved Colorimetric Determination of Salicylic Acid and Its Metabolites in Urine

Clinical Chemistry ◽

10.1093/clinchem/21.8.1167 ◽

1975 ◽

Vol 21 (8) ◽

pp. 1167-1168 ◽

Cited By ~ 20

Author(s):

Nagy A Farid ◽

Gordon S Born ◽

Wayne V Kessler ◽

S M Shaw ◽

W E Lange

Keyword(s):

Salicylic Acid ◽

Sodium Salicylate ◽

Nitrate Solution ◽

Ethylene Dichloride ◽

Ferric Nitrate ◽

Colorimetric Determination ◽

Improved Method ◽

Salicyluric Acid ◽

Sample Recovery

Abstract We describe an improvement in the Levy and Procknal method [J. Pharm. Sci. 57, 1330 (1968)] for determination of salicylic acid and its metabolites in urine. Salicylic acid and salicyluric acid are successively extracted from 1 or 2 ml of urine (acidified with HCl) by two 10-ml portions each of carbon tetrachloride and ethylene dichloride. The extracts of each solvent are shaken with 5 ml of ferric nitrate solution (a 10-fold dilution of 17 g of Fe(NO3)3·9H2O in 1 liter of 70 mmol/liter HNO3). The aqueous phases are centrifuged and their absorbances measured at 530 nm. For total salicylate, 3 ml of urine and 3 ml of HCl are heated in a partially evacuated serum vial at 100 °C for 16 h and then salicylic acid is assayed in the hydrolyzed sample. Recovery of a weighed oral dose of sodium salicylate in urine was 105.4%; it was 127.9% by the Levy and Procknal method for the same sample. The improved method is faster and more accurate.

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