High-throughput single-molecule force spectroscopy for membrane proteins

2008 ◽  
Vol 19 (38) ◽  
pp. 384014 ◽  
Author(s):  
Patrick D Bosshart ◽  
Fabio Casagrande ◽  
Patrick L T M Frederix ◽  
Merce Ratera ◽  
Christian A Bippes ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
High Throughput ◽  
Single Molecule ◽  
Force Spectroscopy ◽  
Single Molecule Force Spectroscopy

scholarly journals Sample preparation method to improve the efficiency of high-throughput single-molecule force spectroscopy

2019 ◽  
Vol 5 (4) ◽  
pp. 176-183 ◽  
Author(s):  
Lei Jin ◽  
Li Kou ◽  
Yanan Zeng ◽  
Chunguang Hu ◽  
Xiaodong Hu
Keyword(s):  
Sample Preparation ◽  
High Throughput ◽  
Single Molecule ◽  
Preparation Method ◽  
Force Spectroscopy ◽  
Experimental Result ◽  
Sample Preparation Method ◽  
Dna Stretching ◽  
Single Molecule Force Spectroscopy ◽  
Preparation Methods

Abstract Inefficient sample preparation methods hinder the performance of high-throughput single-molecule force spectroscopy (H-SMFS) for viscous damping among reactants and unstable linkage. Here, we demonstrated a sample preparation method for H-SMFS systems to achieve a higher ratio of effective target molecules per sample cell by gas-phase silanization and reactant hydrophobization. Digital holographic centrifugal force microscopy (DH-CFM) was used to verify its performance. The experimental result indicated that the DNA stretching success ratio was improved from 0.89% to 13.5%. This enhanced efficiency preparation method has potential application for force-based DNA stretching experiments and other modifying procedures.

Single-Molecule Force Spectroscopy from Nanodiscs: An Assay to Quantify Folding, Stability, and Interactions of Native Membrane Proteins

ACS Nano ◽  
2011 ◽  
Vol 6 (1) ◽  
pp. 961-971 ◽  
Author(s):  
Michael Zocher ◽  
Christian Roos ◽  
Susanne Wegmann ◽  
Patrick D. Bosshart ◽  
Volker Dötsch ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Single Molecule ◽  
Force Spectroscopy ◽  
Single Molecule Force Spectroscopy

Single-Molecule Force Spectroscopy of Membrane Proteins from Membranes Freely Spanning Across Nanoscopic Pores

Nano Letters ◽  
2015 ◽  
Vol 15 (5) ◽  
pp. 3624-3633 ◽  
Author(s):  
Rafayel Petrosyan ◽  
Christian A. Bippes ◽  
Stefan Walheim ◽  
Daniel Harder ◽  
Dimitrios Fotiadis ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Single Molecule ◽  
Force Spectroscopy ◽  
Single Molecule Force Spectroscopy
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