scholarly journals Improves theIn VitroDevelopmental Competence and Reprogramming Efficiency of Cloned Bovine Embryos by Additional Complimentary Cytoplasm

2019 ◽  
Vol 21 (1) ◽  
pp. 51-60 ◽  
Author(s):  
Lianguang Xu ◽  
Ayman Mesalam ◽  
Kyeong-Lim Lee ◽  
Seok-Hwan Song ◽  
Imran Khan ◽  
...  
Keyword(s):  
Bovine Embryos ◽  
Reprogramming Efficiency ◽  
Cloned Bovine

Effects of the removal of cytoplasm on the development of early cloned bovine embryos

2011 ◽  
Vol 126 (1-2) ◽  
pp. 37-44 ◽  
Author(s):  
Song Hua ◽  
Hui Zhang ◽  
Jian Min Su ◽  
Tuo Zhang ◽  
Fu Sheng Quan ◽  
...  
Keyword(s):  
Bovine Embryos ◽  
Cloned Bovine

scholarly journals GLOBAL GENE EXPRESSION PROFILING OF SINGLE, CLONED BOVINE EMBRYOS WITH DIFFERENT DEVELOPMENTAL COMPETENCIES: THE GOOD, THE BAD, AND THE UGLY

2007 ◽  
Vol 77 (Suppl_1) ◽  
pp. 217-217
Author(s):  
Sadie Smith ◽  
Daniel Le Bourhis ◽  
Xavier Vignon ◽  
Yvan Heyman ◽  
Harris Lewin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Global Gene Expression ◽  
Bovine Embryos ◽  
Global Gene Expression Profiling ◽  
Cloned Bovine

Aberrant methylation of donor genome in cloned bovine embryos

10.1038/88903 ◽  
2001 ◽  
Vol 28 (2) ◽  
pp. 173-177 ◽  
Author(s):  
Yong-Kook Kang ◽  
Deog-Bon Koo ◽  
Jung-Sun Park ◽  
Young-Hee Choi ◽  
An-Sik Chung ◽  
...  
Keyword(s):  
Aberrant Methylation ◽  
Bovine Embryos ◽  
Donor Genome ◽  
Cloned Bovine

55 EFFECT OF FUSION-ACTIVATION INTERVAL AND EMBRYO AGGREGATION ON IN VITRO AND IN VIVO DEVELOPMENT OF CLONED BOVINE EMBRYOS PRODUCED BY HANDMADE CLONING

2010 ◽  
Vol 22 (1) ◽  
pp. 185
Author(s):  
R. P. C. Gerger ◽  
F. Forell ◽  
J. C. Mezzalira ◽  
F. Zago ◽  
F. K. Vieira ◽  
...  
Keyword(s):  
Somatic Cell ◽  
High Rate ◽  
Bovine Embryos ◽  
In Vitro Development ◽  
Cloned Bovine ◽  
Vitro Development ◽  
Handmade Cloning ◽  
Activation Intervals

Despite the apparent success of cloning by somatic cell nuclear transfer (SCNT), the efficiency in development to term remains low, with a high rate of losses occurring throughout pregnancy due to faulty reprogramming and conceptus abnormalities. As the ideal fusion-activation interval for optimal nuclear reprogramming after cloning is still ill-defined, the aim of this study was to determine the effect of 2 distinct fusion-activation intervals and embryo aggregation on in vitro development of cloned bovine embryos. Bovine COCs from slaughterhouse ovaries were used after IVM for the production of cloned embryos by handmade cloning, according to our established procedures (Ribeiro et al. 2009 Cloning Stem Cells, in press). Following cumulus and zona removal, oocytes were manually bisected, with hemi-cytoplasts selected by DNA staining. Two hemi-cytoplasts and an adult skin somatic cell were attached and fused with a 15V AC pre-pulse for 5 s, followed by a double 1.2 kV cm-1 DC pulse for 20 μs. Reconstructed embryos were activated in ionomycin exactly at 2 or 4 h post-fusion (2 hpf or 4 hpf), followed by an incubation in 6-DMAP for 4 h. Cloned embryos from both fusion-activation intervals were in vitro-cultured in the well of the well (WOW) system for 7 days, allocating one (1 × 100%) or two (2 × 100%) cloned embryos per WOW. Grade 1 Day-7 blastocysts were transferred to synchronous recipients. Cleavage (Day 2) and blastocyst (Day 7) rates, on a per WOW basis, and pregnancy (Days 30 and 150) rates were compared using the chi-square or the Fisher test, with results from 9 replications summarized in Table 1. Increasing the fusion-activation interval to 4 h decreased cleavage but not blastocyst rates in 1 × 100% embryos. Also, blastocyst rates were lower in 1 × 100% embryos activated 2 h post-fusion. In general, cleavage and blastocysts rates for 2 × 100% embryos (91.5 and 46.0%) were higher than for 1 × 100% embryo counterparts (74.4 and 31.3%), respectively, regardless of the activation time. In addition, blastocyst rates for 4 hpf-activated embryos (50.3%), based on cleavage, were higher than for 2 hpf-activated embryos (38.3%), irrespective of the aggregation scheme. Nonetheless, despite differences in in vitro development, pregnancy rates and conceptus development in the first half of pregnancy were similar between groups. A longer fusion-activation interval (4 hpf) or embryo aggregation (2 × 100%) increased blastocyst yield but did not improve in vivo development and pregnancy maintenance following the transfer to female recipients in cattle. Table 1.In vitro and in vivo development of cloned bovine embryos This study was supported by FAPESP and CAPES, Brazil.

Effects of Insulin-like Growth Factor-1 on Development of Somatic Cell Cloned Bovine Embryos

2016 ◽  
Vol 18 (3) ◽  
pp. 162-170 ◽  
Author(s):  
Pengxiang Qu ◽  
Yanyan Li ◽  
Tengfei Deng ◽  
Dan Jia ◽  
Suzhu Qing ◽  
...  
Keyword(s):  
Growth Factor ◽  
Somatic Cell ◽  
Insulin Like Growth Factor ◽  
Bovine Embryos ◽  
Cloned Bovine

scholarly journals Influence of oocyte nuclei on demethylation of donor genome in cloned bovine embryos

FEBS Letters ◽  
2001 ◽  
Vol 499 (1-2) ◽  
pp. 55-58 ◽  
Author(s):  
Yong-Kook Kang ◽  
Deog-Bon Koo ◽  
Jung-Sun Park ◽  
Young-Hee Choi ◽  
Kyung-Kwang Lee ◽  
...  
Keyword(s):  
Bovine Embryos ◽  
Donor Genome ◽  
Cloned Bovine

Effects of Co-culture of Cumulus Oocyte Complexes with Denuded Oocytes During In Vitro Maturation on the Developmental Competence of Cloned Bovine Embryos

2015 ◽  
Vol 50 (2) ◽  
pp. 292-298 ◽  
Author(s):  
A-N Ha ◽  
M Fakruzzaman ◽  
K-L Lee ◽  
J-I Bang ◽  
G-K Deb ◽  
...  
Keyword(s):  
In Vitro Maturation ◽  
Developmental Competence ◽  
Bovine Embryos ◽  
Cloned Bovine

scholarly journals Effect of Cryoprotectant Composition on In Vitro Viability of In Vitro Fertilized and Cloned Bovine Embryos Following Vitrification and In-Straw Dilution

2007 ◽  
Vol 53 (4) ◽  
pp. 963-969 ◽  
Author(s):  
Masayasu TANIGUCHI ◽  
Akiko IKEDA ◽  
Eri ARIKAWA ◽  
Pimprapar WONGSRIKEAO ◽  
Budiyanto AGUNG ◽  
...  
Keyword(s):  
Bovine Embryos ◽  
Cloned Bovine

Cytogenetic analysis of diploidy in cloned bovine embryos using an improved air-dry karyotyping method

2005 ◽  
Vol 63 (9) ◽  
pp. 2434-2444 ◽  
Author(s):  
Guang-Peng Li ◽  
Ying Liu ◽  
Kenneth L. White ◽  
Thomas D. Bunch
Keyword(s):  
Cytogenetic Analysis ◽  
Bovine Embryos ◽  
Cloned Bovine
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