A Screening Test for Detection of Elevated Serum Glutamic Oxalacetic Transaminase Activity

1967 ◽  
Vol 47 (4_ts) ◽  
pp. 541-544 ◽  
Author(s):  
J. Lamar Rencher ◽  
Myrton F. Beeler
Keyword(s):  
Screening Test ◽  
Elevated Serum ◽  
Transaminase Activity ◽  
Glutamic Oxalacetic Transaminase ◽  
Serum Glutamic Oxalacetic Transaminase

Sources of Variation in a Standardized and a Semi-micro Procedure for the Spectrophotometric Assay of Serum Glutamic-Oxalacetic Transaminase Concentration

1958 ◽  
Vol 4 (5) ◽  
pp. 392-408 ◽  
Author(s):  
A J Schneider ◽  
Myron J Willis
Keyword(s):  
Arrhenius Equation ◽  
Spectrophotometric Assay ◽  
Light Path ◽  
Transaminase Activity ◽  
Arrhenius Law ◽  
Glutamic Oxalacetic Transaminase ◽  
Sources Of Variation ◽  
The Mean ◽  
Serum Glutamic Oxalacetic Transaminase ◽  
Temperature Factors

Abstract 1. Both a standard method and a semi-micro method for the spectrophotometric assay of serum glutamic-oxalacetic transaminase (S-GOT) concentrations have been described. 2. Either procedure is associated with an error defined by a factor of less than 1.03. 3. The temperature dependence of the rate of transamination was shown to follow Arrhenius' law over the range of temperature from 25° to 38°. 4. A tabulation of temperature factors calculated from the derived Arrhenius equation was presented. These factors permit correction of rates observed at temperature T to rates expected at 32°. 5. A comparison of normal S-GOT values from various sources was made, with correction for temperature differences. Based on 779 values from four different laboratories, the combined mean for adults was 21.9. 6. A standard unit of transaminase activity was defined and referred to as a Karmen unit. A Karmen unit represents that amount of transaminase in 1 ml. of sample which will cause a decrease in optical density at 340 mµ of 0.001 per minute at a temperature of 32°, an effective light path of 1 cm., and a volume of test solution of 3 ml. According to this definition, the mean normal adult S-GOT concentration is 21.9 Karmen units. The practical upper limit of normal will be defined in another publication.

A Study of the Colorimetric Dinitrophenylhydrazine Method for the Determination of Serum Glutamic Oxalacetic Transaminase Activity

1966 ◽  
Vol 12 (4) ◽  
pp. 217-225 ◽  
Author(s):  
J S Annino
Keyword(s):  
Enzyme Activity ◽  
Transaminase Activity ◽  
Reagent Blank ◽  
Glutamic Oxalacetic Transaminase ◽  
Color Development ◽  
High Enzyme ◽  
Serum Glutamic Oxalacetic Transaminase ◽  
The Relationship ◽  
High Enzyme Activity

Abstract Study of the colorimetric transaminase method of Reitman and Frankel for the determination of serum glutamic oxalacetic transaminase activity revealed the following: (1) although maximum absorption occurs at 444 mµ, absorbance readings at 505 mµ gave satisfactory results; (2) color development is immediate and the color is stable for at least 1 hr.; (3) a pyruvate calibration standard may be used; (4) sample blanks are not usually necessary; (5) a reagent blank should accompany each group of analyses and should be used as a photometric reference; (6) the relationship between dilution and enzyme activity is linear; and (7) although the relationship between incubation time and activity is not exactly linear, a factor has been determined to permit the use of a 12-min. incubation period with samples showing high enzyme activity.

Detection of Hepatitis Carriers by Serum Glutamic Oxalacetic Transaminase Activity*

1960 ◽  
Vol 39 (3) ◽  
pp. 309???314
Author(s):  
NILS U. BANG ◽  
PAUL RUEGSEGGER ◽  
ALLYN B. LEY ◽  
JOHN S. LA DUE
Keyword(s):  
Transaminase Activity ◽  
Glutamic Oxalacetic Transaminase ◽  
Serum Glutamic Oxalacetic Transaminase
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