scholarly journals Evaluation of potential risks associated with recombination in transgenic plants expressing viral sequences

2008 ◽  
Vol 89 (1) ◽  
pp. 327-335 ◽  
Author(s):  
Camilla Turturo ◽  
Arianna Friscina ◽  
Stéphane Gaubert ◽  
Mireille Jacquemond ◽  
Jeremy R. Thompson ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Viral Rna ◽  
Rna Recombination ◽  
Recombinant Viruses ◽  
Tomato Aspermy Virus ◽  
Potential Risks ◽  
Viral Sequences

Virus-resistant transgenic plants have been created primarily through the expression of viral sequences. It has been hypothesized that recombination between the viral transgene mRNA and the RNA of an infecting virus could generate novel viruses. As mRNA/viral RNA recombination can occur in virus-resistant transgenic plants, the key to testing this risk hypothesis is to compare the populations of recombinant viruses generated in transgenic and non-transgenic plants. This has been done with two cucumoviral systems, involving either two strains of cucumber mosaic virus (CMV), or CMV and the related tomato aspermy virus (TAV). Although the distribution of the sites of recombination in the CMV/CMV and TAV/CMV systems was quite different, equivalent populations of recombinant viruses were observed in both cases. These results constitute the first comparison of the populations of recombinants in transgenic and non-transgenic plants, and suggest that there is little risk of emergence of recombinant viruses in these plants, other than those that could emerge in non-transgenic plants.

scholarly journals Deep Sequencing of Recombinant Virus Populations in Transgenic and Nontransgenic Plants Infected with Cucumber mosaic virus

2013 ◽  
Vol 26 (7) ◽  
pp. 801-811 ◽  
Author(s):  
Marco Morroni ◽  
Mireille Jacquemond ◽  
Mark Tepfer
Keyword(s):  
Transgenic Plants ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
De Novo ◽  
Recombinant Viruses ◽  
Viral Genomes ◽  
Large Deletions ◽  
Cp Gene ◽  
Viral Sequences ◽  
Virus Variability

Recombination is a major source of virus variability, and the question of whether novel recombinant viruses would emerge in transgenic plants expressing viral sequences has been a biosafety issue. We describe the results of pyrosequencing the recombinant viral RNAs appearing in transgenic plants expressing the coat protein (CP) gene and 3′ noncoding region of Cucumber mosaic virus RNA3, as well as in nontransgenic controls. The populations of recombinants in both transgenic and nontransgenic plants were similar to those previously described from Sanger sequencing but many more recombinant types were observed, including a novel class of large deletions removing all or nearly the entire CP gene. These results show that populations of recombinant viral genomes arising de novo can be characterized in detail by pyrosequencing, and confirm that the transgenic plants did not harbor novel recombinants of biosafety concern.

scholarly journals Comparison of properties of particles of Cucumber mosaic virus and Tomato aspermy virus based on the analysis of molecular surfaces of capsids

2006 ◽  
Vol 87 (7) ◽  
pp. 2073-2083 ◽  
Author(s):  
Luis F. Pacios ◽  
Fernando García-Arenal
Keyword(s):  
Coat Protein ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Capsid Proteins ◽  
Particle Structure ◽  
External Region ◽  
Molecular Surfaces ◽  
Recombinant Viruses ◽  
Tomato Aspermy Virus ◽  
Particle Stability

The plant RNA viruses Cucumber mosaic virus (CMV) and Tomato aspermy virus (TAV) (genus Cucumovirus) have similar icosahedral particles, the crystal structures of which have been reported recently. Similarity in particle structure agrees with reports of stable capsids assembled from their capsid proteins and of viable recombinant viruses with chimeric capsid proteins derived from CMV and TAV. However, differences between the cucumoviruses have been reported for physicochemical properties. Here, structural and electrostatic features of the molecular surfaces are studied to investigate their relationship with these observations. Two coat-protein recombinants with structures modelled by taking CMV and TAV as templates were also included in the analysis. Results show that there exists an external region of negative electrostatic potential that has arisen from strictly conserved charged residues situated near the external HI loop of the subunits in the capsomers. This negative domain surrounds the fivefold and quasi-sixfold axes and locates above regions of positive potential that extend to cover, nearly homogeneously, the inner surface of capsids, where interaction with encapsidated RNA occurs. Differences between the outer electrostatic distributions in CMV and TAV explain the distinct response of both viruses to variations in physicochemical conditions required for particle stability and are essential to rationalize the biological activity of the coat-protein recombinants, in spite of their seemingly distinct electrostatic characteristics.

Cucumber mosaic virus-associated RNA 5 IX. The overtaking of viral RNA synthesis by CARNA 5 and dsCARNA 5 in tobacco

Virology ◽  
1982 ◽  
Vol 122 (1) ◽  
pp. 147-157 ◽  
Author(s):  
P. Piazzolla ◽  
M.E. Tousignant ◽  
J.M. Kaper
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Rna Synthesis ◽  
Viral Rna

Differential interactions among strains of tomato aspermy virus and satellite RNAs of cucumber mosaic virus

Virology ◽  
1992 ◽  
Vol 186 (2) ◽  
pp. 475-480 ◽  
Author(s):  
Enrique Moriones ◽  
Isabel Diaz ◽  
Emilio Rodriguez-Cerezo ◽  
Aurora Fraile ◽  
Fernando Garcia-Arenal
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Tomato Aspermy Virus ◽  
Satellite Rnas

Anti-sense RNAs of cucumber mosaic virus in transgenic plants assessed for control of the virus

1988 ◽  
Vol 11 (4) ◽  
pp. 463-471 ◽  
Author(s):  
M. Ali Rezaian ◽  
Ken G. M. Skene ◽  
Jeff G. Ellis
Keyword(s):  
Transgenic Plants ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus
Sign in / Sign up

Export Citation Format

Share Document