Satellite-RNA-mediated resistance to cucumber mosaic virus in transgenic plants of hot pepper (Capsicum annuum cv. Golden Tower)

1997 ◽  
Vol 16 (12) ◽  
pp. 825-830 ◽  
Author(s):  
S. J. Kim ◽  
S. J. Lee ◽  
B.-D. Kim ◽  
K.-H. Paek
Keyword(s):  
Transgenic Plants ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Capsicum Annuum ◽  
Hot Pepper ◽  
Satellite Rna

Virus resistance in transgenic plants that express cucumber mosaic virus satellite RNA

Nature ◽  
1987 ◽  
Vol 328 (6133) ◽  
pp. 799-802 ◽  
Author(s):  
Bryan D. Harrison ◽  
Michael A. Mayo ◽  
David C. Baulcombe
Keyword(s):  
Transgenic Plants ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Virus Resistance ◽  
Satellite Rna

Characterisation of a satellite RNA of Cucumber mosaic virus that induces chlorosis in Capsicum annuum

Virus Genes ◽  
2011 ◽  
Vol 43 (1) ◽  
pp. 111-119 ◽  
Author(s):  
Seung-Kook Choi ◽  
Yong-Woon Jeon ◽  
Ju-Yeon Yoon ◽  
Jang-Kyung Choi
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Capsicum Annuum ◽  
Satellite Rna

scholarly journals Transgenic Tomato Plants Expressing Satellite RNA Are Tolerant to Some Strains of Cucumber Mosaic Virus

1994 ◽  
Vol 119 (3) ◽  
pp. 642-647 ◽  
Author(s):  
P.B. McGarvey ◽  
M.S. Montasser ◽  
J.M. Kaper
Keyword(s):  
Transgenic Plants ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Unit Length ◽  
Transgenic Tomato ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Satellite Rna ◽  
Tomato Plants ◽  
Transgenic Tomato Plants

Transgenic tomato plants (Lycopersicon esculentum Mill.) expressing cucumber mosaic virus (CMV) satellite RNA fused to a gene for β-glucuronidase were produced using Agrobacterium-mediated transformation. The R1 progeny of self-crossed R0 plants were challenge-inoculated with virion or RNA preparations of CMV or tomato aspermy virus (TAV). The transgenic plants challenged with CMV-1 showed mild disease symptoms in the first 2 weeks postchallenge followed by a decrease in symptoms, resulting in little difference between the transgenic and uninfected control group by the fourth week. Enzyme-linked immunosorbent assay results showed about a 10-fold decrease in virus accumulation in the transgenic plants compared to controls. Tolerance was evident only in plants that contained the recombinant insert and produced mature unit-length satellite RNA after CMV infection. Plants challenged with TAV showed no significant tolerance to virus-induced symptoms.

scholarly journals Protective Effect of a Satellite RNA Expressed in Transgenic Plants on Disease Incidence after Inoculation of Cucumber Mosaic Virus.

1994 ◽  
Vol 60 (2) ◽  
pp. 228-232 ◽  
Author(s):  
Chikara MASUTA ◽  
Yumiko HAYASHI ◽  
Masashi SUZUKI ◽  
Shigeru KUWATA ◽  
Yoichi TAKANAMI ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Protective Effect ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Disease Incidence ◽  
Satellite Rna

scholarly journals Delay of Disease Development in Transgenic Petunia Plants Expressing Cucumber Mosaic Virus I17N-Satellite RNA

1995 ◽  
Vol 120 (2) ◽  
pp. 353-359 ◽  
Author(s):  
Shin Je Kim ◽  
Kyung-Hee Paek ◽  
Byung-Dong Kim
Keyword(s):  
Transgenic Plants ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Unit Length ◽  
Cauliflower Mosaic Virus ◽  
35S Promoter ◽  
Disease Development ◽  
Satellite Rna ◽  
Camv 35S ◽  
Transgenic Petunia

A cDNA clone of cucumber mosaic virus (CMV) 117 N-satellite RNA driven by the cauliflower mosaic virus (CaMV) 35S transcript promoter, was stably integrated into the genome of Petunia hybrida `Bluepicoti' tissues by Agrobacterium tumefaciens Ti plasmid-mediated transformation. Transgenic plants producing CMV satellite RNA showed delayed disease development when inoculated with CMV-Y, a helper virus for the I17N-satellite RNA. Furthermore, transgenic petunia plants showed delayed disease development against tobacco mosaic virus (TMV), a tobamovirus not related to CMV. Northern blot analysis revealed that large amounts of unit length satellite RNA (335 bp) were produced in CMV-infected transgenic petunia plants; whereas, mainly transcripts driven by the CaMV 35S promoter (approximately 1 kb) were produced in TMV-infected transgenic plants. SDS-PAGE and Western blotting showed that symptom reduction was correlated with a reduction in the amount of viral coat protein in transgenic plants.

scholarly journals Nuevos registros de aislamientos del Cucumber mosaic virus y su RNA satélite en Colima, México

2019 ◽  
Vol 37 (2) ◽  
Author(s):  
Pedro Valadez-Ramírez ◽  
Javier Paz-Román ◽  
Salvador Guzmán-González ◽  
Marco Tulio Buenrostro-Nava ◽  
Daniel Leobardo Ochoa-Martínez
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Solanum Lycopersicum ◽  
Capsicum Annuum ◽  
Catharanthus Roseus ◽  
Cucumis Melo ◽  
Rt Pcr ◽  
Impacto Económico

El <em>Cucumber mosaic virus</em> (CMV) ocasiona una de las enfermedades virales más importantes a nivel mundial en plantas silvestres y cultivadas. En México son pocos los estudios que se han abordado con este virus, y dada su amplia gama de hospedantes e impacto económico, es necesario contar con mayor información de su presencia y distribución en zonas de importancia agrícola como las del estado de Colima. En este trabajo, se reportan nuevos aislamientos del CMV identificados por RT-PCR, secuenciación de DNA y su análisis filogenético: CMV-Vin en vinca (<em>Catharanthus roseus</em>), CMV-Chi en chile jalapeño (<em>Capsicum annuum</em>) y CMV-Tom en tomate saladette (<em>Solanum lycopersicum</em>). Se confirmó, además, la presencia del CMV en melón cantaloupe (<em>Cucumis melo</em>) (CMV-Mel). Los aislamientos CMV-Vin, CMV-Chi y CMV-Mel agruparon en el subgrupo IB, mientras que CMV-Tom agrupó en el subgrupo IA de CMV. De estos aislamientos, sólo CMV-Vin evidenció la presencia de un RNA satélite (satRNA Vin) sin dominio necrogénico. Este es el primer reporte de la presencia del CMV en vinca, chile y tomate y de un RNA satélite en vinca en Colima, México.

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