SIRT Combines Homologous Recombination, Site-Specific Integration, and Bacterial Recombineering for Targeted Mutagenesis in Drosophila

2009 ◽  
Vol 2009 (6) ◽  
pp. pdb.prot5236-pdb.prot5236 ◽  
Author(s):  
G. Gao ◽  
N. Wesolowska ◽  
Y. S. Rong
Keyword(s):  
Homologous Recombination ◽  
Targeted Mutagenesis ◽  
Recombination Site ◽  
Site Specific ◽  
Site Specific Integration

scholarly journals Erratum: Efficient site-specific integration in Plasmodium falciparum chromosomes mediated by mycobacteriophage Bxb1 integrase

2006 ◽  
Vol 3 (9) ◽  
pp. 763-763
Author(s):  
Louis J Nkrumah ◽  
Rebecca A Muhle ◽  
Pedro A Moura ◽  
Pallavi Ghosh ◽  
Graham F Hatfull ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Site Specific ◽  
Site Specific Integration

scholarly journals Transcriptional Analysis of the Adeno-Associated Virus Integration Site

2009 ◽  
Vol 83 (23) ◽  
pp. 12512-12525 ◽  
Author(s):  
Nathalie Dutheil ◽  
Els Henckaerts ◽  
Erik Kohlbrenner ◽  
R. Michael Linden
Keyword(s):  
Transcriptional Activity ◽  
Integration Site ◽  
Regulatory Elements ◽  
Transcriptional Analysis ◽  
Start Codon ◽  
Adeno Associated Virus ◽  
Site Specific ◽  
Complex Interactions ◽  
Site Specific Integration ◽  
Virus Integration

ABSTRACT The nonpathogenic human adeno-associated virus type 2 (AAV-2) has adopted a unique mechanism to site-specifically integrate its genome into the human MBS85 gene, which is embedded in AAVS1 on chromosome 19. The fact that AAV has evolved to integrate into this ubiquitously transcribed region and that the chromosomal motifs required for integration are located a few nucleotides upstream of the translation initiation start codon of MBS85 suggests that the transcriptional activity of MBS85 might influence site-specific integration and thus might be involved in the evolution of this mechanism. In order to begin addressing this question, we initiated the characterization of the human MBS85 promoter region and compared its transcriptional activity to that of the AAV-2 p5 promoter. Our results clearly indicate that AAVS1 is defined by a complex transcriptional environment and that the MBS85 promoter shares key regulatory elements with the viral p5 promoter. Furthermore, we provide evidence for bidirectional MBS85 promoter activity and demonstrate that the minimal motifs required for AAV site-specific integration are present in the 5′ untranslated region of the gene and play a posttranscriptional role in the regulation of MBS85 expression. These findings should provide a framework to further elucidate the complex interactions between the virus and its cellular host in this unique pathway to latency.

Site-specific integration of rotavirus VP6 gene in rabbit β-casein locus by CRISPR/Cas9 system

2019 ◽  
Vol 55 (8) ◽  
pp. 586-597 ◽  
Author(s):  
Hongli Li ◽  
Zhipeng Li ◽  
Ning Xiao ◽  
Xiaoping Su ◽  
Shanshan Zhao ◽  
...  
Keyword(s):  
Site Specific ◽  
Site Specific Integration ◽  
Vp6 Gene ◽  
Rotavirus Vp6

scholarly journals Cloning of Frankia species putative tRNA(Pro) genes and their efficacy for pSAM2 site-specific integration in Streptomyces lividans.

1994 ◽  
Vol 60 (12) ◽  
pp. 4279-4283 ◽  
Author(s):  
M T Alegre ◽  
B Cournoyer ◽  
J M Mesas ◽  
M Guérineau ◽  
P Normand ◽  
...  
Keyword(s):  
Streptomyces Lividans ◽  
Site Specific ◽  
Site Specific Integration
Sign in / Sign up

Export Citation Format

Share Document