scholarly journals ZOO: an automatic data-collection system for high-throughput structure analysis in protein microcrystallography

2019 ◽  
Vol 75 (2) ◽  
pp. 138-150 ◽  
Author(s):  
Kunio Hirata ◽  
Keitaro Yamashita ◽  
Go Ueno ◽  
Yoshiaki Kawano ◽  
Kazuya Hasegawa ◽  
...  

Owing to the development of brilliant microfocus beamlines, rapid-readout detectors and sample changers, protein microcrystallography is rapidly becoming a popular technique for accessing structural information from complex biological samples. However, the method is time-consuming and labor-intensive and requires technical expertise to obtain high-resolution protein crystal structures. At SPring-8, an automated data-collection system named ZOO has been developed. This system enables faster data collection, facilitates advanced data-collection and data-processing techniques, and permits the collection of higher quality data. In this paper, the key features of the functionality put in place on the SPring-8 microbeam beamline BL32XU are described and the major advantages of this system are outlined. The ZOO system will be a major driving force in the evolution of the macromolecular crystallography beamlines at SPring-8.

2013 ◽  
Vol 779-780 ◽  
pp. 878-889
Author(s):  
Su Ping Chen ◽  
Dai Zong Liu

This research explores the application of available information from Automated Data Collection Systems (ADC) with a focus on bus passenger Origin-Destination (OD) matrices inference, using Chongqing as a case study. It demonstrates the feasibility and ease of applying to infer bus passengers boarding and alighting locations if boarding information is available from neither Automatic Fare Collection System (AFC) nor GPS data, and is the first known attempt to create an algorism to use stop Geographic Information System (GIS) and dual-direction bus stop group to estimate the boarding location and traveling direction when GPS data cannot provide the arriving information at bus stops. The results of this research have been practically applied to a full-size bus network in Chongqing, and can be easily implemented to other Chinese cities as Chongqing presents most popular data structure of ADC in China.


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