scholarly journals Combining bleach and mild predigestion improves ancient DNA recovery from bones

2016 ◽  
Vol 17 (4) ◽  
pp. 742-751 ◽  
Author(s):  
Sanne Boessenkool ◽  
Kristian Hanghøj ◽  
Heidi M. Nistelberger ◽  
Clio Der Sarkissian ◽  
Agata T. Gondek ◽  
...  
Keyword(s):  
Ancient Dna ◽  
Dna Recovery

Aspartic acid racemization variability in ancient human remains: implications in the prediction of ancient DNA recovery

2009 ◽  
Vol 36 (4) ◽  
pp. 965-972 ◽  
Author(s):  
E. Fernández ◽  
J.E. Ortiz ◽  
A. Pérez-Pérez ◽  
E. Prats ◽  
D. Turbón ◽  
...  
Keyword(s):  
Aspartic Acid ◽  
Ancient Dna ◽  
Human Remains ◽  
Dna Recovery ◽  
Aspartic Acid Racemization

scholarly journals Ancient Plant Genomics in Archaeology, Herbaria, and the Environment

2020 ◽  
Vol 71 (1) ◽  
pp. 605-629 ◽  
Author(s):  
Logan Kistler ◽  
Vanessa C. Bieker ◽  
Michael D. Martin ◽  
Mikkel Winther Pedersen ◽  
Jazmín Ramos Madrigal ◽  
...  
Keyword(s):  
Ancient Dna ◽  
Plant Evolution ◽  
Genomic Research ◽  
Degraded Dna ◽  
Plant Domestication ◽  
Plant Genomics ◽  
Dna Recovery ◽  
Plant Research

The ancient DNA revolution of the past 35 years has driven an explosion in the breadth, nuance, and diversity of questions that are approachable using ancient biomolecules, and plant research has been a constant, indispensable facet of these developments. Using archaeological, paleontological, and herbarium plant tissues, researchers have probed plant domestication and dispersal, plant evolution and ecology, paleoenvironmental composition and dynamics, and other topics across related disciplines. Here, we review the development of the ancient DNA discipline and the role of plant research in its progress and refinement. We summarize our understanding of long-term plant DNA preservation and the characteristics of degraded DNA. In addition, we discuss challenges in ancient DNA recovery and analysis and the laboratory and bioinformatic strategies used to mitigate them. Finally, we review recent applications of ancient plant genomic research.

scholarly journals Human Auditory Ossicles as an Alternative Optimal Source of Ancient DNA

2019 ◽  
Author(s):  
Kendra Sirak ◽  
Daniel Fernandes ◽  
Olivia Cheronet ◽  
Eadaoin Harney ◽  
Matthew Mah ◽  
...  
Keyword(s):  
Ancient Dna ◽  
Dna Analysis ◽  
Substantial Reduction ◽  
Future Research ◽  
Alternative Source ◽  
Dna Recovery ◽  
Auditory Ossicles ◽  
Genetic Landscape ◽  
Ancient Dna Analysis ◽  
The Rich

ABSTRACTDNA recovery from ancient human remains has revolutionized our ability to reconstruct the genetic landscape of the past. Ancient DNA research has benefited from the identification of skeletal elements, such as the cochlear part of the osseous inner ear, that provide optimal contexts for DNA preservation; however, the rich genetic information obtained from the cochlea must be counterbalanced against the loss of valuable morphological information caused by its sampling. Motivated by similarities in developmental processes and histological properties between the cochlea and auditory ossicles, we evaluated the efficacy of ossicles as an alternative source of ancient DNA. We demonstrate that ossicles perform comparably to the cochlea in terms of DNA recovery, finding no substantial reduction in data quality, quantity, or authenticity across a range of preservation conditions. Ossicles can be sampled from intact skulls or disarticulated petrous bones without damage to surrounding bone, and we argue that, when available, they should be selected over the cochlea to reduce damage to skeletal integrity. These results identify a second optimal skeletal element for ancient DNA analysis and add to a growing toolkit of sampling methods that help to better preserve skeletal remains for future research while maximizing the likelihood that ancient DNA analysis will produce useable results.

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