Normal Human Bone Marrow Cultures in Vitro: Cellular Composition and Maturation of the Granulocytic Colonies

1977 ◽  
Vol 37 (1) ◽  
pp. 127-136 ◽  
Author(s):  
C. Dao ◽  
D. Metcalf ◽  
R. Zittoun ◽  
G. Bilski-Pasquier
Keyword(s):  
Bone Marrow ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Cellular Composition ◽  
Bone Marrow Cultures ◽  
Normal Human ◽  
Human Bone Marrow Cultures ◽  
Normal Human Bone Marrow ◽  
Marrow Cultures

scholarly journals No stimulative effect of adipocytes on hematopoiesis in long-term human bone marrow cultures

Blood ◽  
1983 ◽  
Vol 61 (4) ◽  
pp. 770-774
Author(s):  
I Touw ◽  
B Lowenberg
Keyword(s):  
Bone Marrow ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Adherent Cell ◽  
Murine Bone Marrow ◽  
Bone Marrow Cultures ◽  
Human Bone Marrow Cultures ◽  
Marrow Cultures

Long-term cultures of human bone marrow were established for 5–13 wk to study the role of adipocytes in sustaining hematopoiesis. At weekly intervals, the numbers of nucleated cells and granulocyte-macrophage progenitor cells (GM-CFU) in culture were estimated in relation to the numbers of fat-containing cells present in the adherent stroma layer. In these quantifications, the numbers of GM-CFU trapped in the adherent cell layer were considered separately. It was found that the presence of adipocytes did not correlate with more active hematopoiesis. Fat cells appeared at late stages when successful cultures were being exhausted or early in cultures with poor activity. These observations suggest that human marrow continuous hematopoiesis in vitro, unlike hematopoiesis in the analogous murine bone marrow cultures, does not depend on the presence of adipocytes.

scholarly journals Effects of testosterone and erythropoietin on erythroid colony formation in human bone marrow cultures

Blood ◽  
1975 ◽  
Vol 45 (5) ◽  
pp. 665-670 ◽  
Author(s):  
Y Moriyama ◽  
JW Fisher
Keyword(s):  
Bone Marrow ◽  
Colony Formation ◽  
Methyl Cellulose ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Erythroid Cell ◽  
Bone Marrow Cultures ◽  
Human Bone Marrow Cultures ◽  
Marrow Cultures

Abstract The effects of testosterone and erythropoietin (ESF) on erythroid colony formation in normal human bone marrow cultures were studied in vitro using a methyl cellulose gel system. Testosterone was found to produce a significant increase in erythroid colony formation at concentrations of 10–4–10-4M in vitro. In this system, the numbers of erythroid colonies formed per plate increased in direct proportion to the increase in the number of erythroid precursors inoculated as well as to the increase in the dose of ESF in vitro. In addition, a synergistic effect of a combination of testosterone and ESF on erythroid colony formation was seen when ESF was present at high concentrations. These data suggest that a greater number of erythropoietin-responsive cells are available for ESF to differentiate into the nucleated erythroid cell line in the presence of testosterone, indicating that the effect of a combination of testosterone and ESF is greater in enhancing erythropoiesis than the additive effects of either agent alone.

scholarly journals No stimulative effect of adipocytes on hematopoiesis in long-term human bone marrow cultures

Blood ◽  
1983 ◽  
Vol 61 (4) ◽  
pp. 770-774 ◽  
Author(s):  
I Touw ◽  
B Lowenberg
Keyword(s):  
Bone Marrow ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Adherent Cell ◽  
Murine Bone Marrow ◽  
Bone Marrow Cultures ◽  
Human Bone Marrow Cultures ◽  
Marrow Cultures

Abstract Long-term cultures of human bone marrow were established for 5–13 wk to study the role of adipocytes in sustaining hematopoiesis. At weekly intervals, the numbers of nucleated cells and granulocyte-macrophage progenitor cells (GM-CFU) in culture were estimated in relation to the numbers of fat-containing cells present in the adherent stroma layer. In these quantifications, the numbers of GM-CFU trapped in the adherent cell layer were considered separately. It was found that the presence of adipocytes did not correlate with more active hematopoiesis. Fat cells appeared at late stages when successful cultures were being exhausted or early in cultures with poor activity. These observations suggest that human marrow continuous hematopoiesis in vitro, unlike hematopoiesis in the analogous murine bone marrow cultures, does not depend on the presence of adipocytes.

Studies on normal human bone marrow cultures in controlled environment thin-film culture systems

In Vitro ◽  
1976 ◽  
Vol 12 (5) ◽  
pp. 363-372 ◽  
Author(s):  
Salman Gailani ◽  
William F. McLimans ◽  
Annie Nussbaum ◽  
Frances Robinson ◽  
Oliver Roholt
Keyword(s):  
Thin Film ◽  
Bone Marrow ◽  
Human Bone ◽  
Controlled Environment ◽  
Film Culture ◽  
Bone Marrow Cultures ◽  
Normal Human ◽  
Human Bone Marrow Cultures ◽  
Normal Human Bone Marrow ◽  
Marrow Cultures

scholarly journals Effects of testosterone and erythropoietin on erythroid colony formation in human bone marrow cultures

Blood ◽  
1975 ◽  
Vol 45 (5) ◽  
pp. 665-670 ◽  
Author(s):  
Y Moriyama ◽  
JW Fisher
Keyword(s):  
Bone Marrow ◽  
Colony Formation ◽  
Methyl Cellulose ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Erythroid Cell ◽  
Bone Marrow Cultures ◽  
Human Bone Marrow Cultures ◽  
Marrow Cultures

The effects of testosterone and erythropoietin (ESF) on erythroid colony formation in normal human bone marrow cultures were studied in vitro using a methyl cellulose gel system. Testosterone was found to produce a significant increase in erythroid colony formation at concentrations of 10–4–10-4M in vitro. In this system, the numbers of erythroid colonies formed per plate increased in direct proportion to the increase in the number of erythroid precursors inoculated as well as to the increase in the dose of ESF in vitro. In addition, a synergistic effect of a combination of testosterone and ESF on erythroid colony formation was seen when ESF was present at high concentrations. These data suggest that a greater number of erythropoietin-responsive cells are available for ESF to differentiate into the nucleated erythroid cell line in the presence of testosterone, indicating that the effect of a combination of testosterone and ESF is greater in enhancing erythropoiesis than the additive effects of either agent alone.

Formation of Multinucleated Cells that Respond to Osteotropic Hormones in Long Term Human Bone Marrow Cultures*

Endocrinology ◽  
1987 ◽  
Vol 120 (6) ◽  
pp. 2326-2333 ◽  
Author(s):  
B. R. MACDONALD ◽  
N. TAKAHASHI ◽  
L. M. MCMANUS ◽  
J. HOLAHAN, ◽  
G. R. MUNDY ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Multinucleated Cells ◽  
Bone Marrow Cultures ◽  
Human Bone Marrow Cultures ◽  
Marrow Cultures
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