Monoclonal antibody-based ELISA to quantify the major allergen of Artemisia vulgaris pollen, Art v 1

Allergy ◽  
2004 ◽  
Vol 59 (9) ◽  
pp. 995-1001 ◽  
Author(s):  
L. Jimeno ◽  
O. Duffort ◽  
C. Serrano ◽  
D. Barber ◽  
F. Polo
Keyword(s):  
Monoclonal Antibody ◽  
Major Allergen ◽  
Artemisia Vulgaris ◽  
Art V 1

scholarly journals Chemical modification of Art v 1, a major mugwort pollen allergen, by cis-aconitylation and citraconylation

2009 ◽  
Vol 74 (4) ◽  
pp. 359-366 ◽  
Author(s):  
Dragana Stanic ◽  
Lidija Burazer ◽  
Marija Gavrovic-Jankulovic ◽  
Ratko Jankov ◽  
Tanja Cirkovic-Velickovic
Keyword(s):  
Specific Immunotherapy ◽  
Major Allergen ◽  
Artemisia Vulgaris ◽  
Amino Groups ◽  
Ph Values ◽  
Acidic Conditions ◽  
Citraconic Anhydride ◽  
Dimethylmaleic Anhydride ◽  
Derivatives Of ◽  
Art V 1

Art v 1 is the major allergen of mugwort (Artemisia vulgaris) pollen, a significant cause of hay fever all over Europe. Specific immunotherapy is the only treatment modality for allergic disease. Application of modified allergens makes the treatment safer and more efficient. In this work, two out of three (citraconic anhydride, cis-aconitic anhydride, 2,3-dimethylmaleic anhydride) tested anhydrides were proven to be suitable for chemical modifications of allergens. Art v 1 was modified by cis-aconitylation and citraconylation in order to obtain derivatives of Art v 1 that may be suitable for further immunological testing. Acylation of Art v 1 gave derivatives (caaArt v 1 and citArt v 1) with about 80 % modified amino groups. The derivatives were in the monomeric form and had dramatically reduced pI values. Both derivatives were relatively stable at neutral pH values, while the acyl groups undergo hydrolysis under acidic conditions. Modification of allergens by cis-aconitylation and citraconylation could be a new tool for obtaining allergoids.

Monoclonal Antibody-Based ELISA to Quantify the Major Allergen of Cynodon dactylon (Bermuda Grass) Pollen, Cyn d 1

2004 ◽  
Vol 135 (4) ◽  
pp. 277-283 ◽  
Author(s):  
O. Duffort ◽  
B. Calabozo ◽  
R. González ◽  
J.A. Carpizo ◽  
D. Barber ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Grass Pollen ◽  
Cynodon Dactylon ◽  
Major Allergen ◽  
Bermuda Grass ◽  
Bermuda Grass Pollen

Art v 1, the major allergen of mugwort pollen, is a modular glycoprotein with a defensin‐like and a hydroxyproline‐rich domain

2002 ◽  
Vol 17 (1) ◽  
pp. 106-108 ◽  
Author(s):  
Martin Himly ◽  
Beatrice Jahn‐Schmid ◽  
Azra Dedic ◽  
Peter Kelemen ◽  
Nicole Wopfner ◽  
...  
Keyword(s):  
Major Allergen ◽  
Rich Domain ◽  
Art V 1

scholarly journals Localization of the Major Allergen Bet V 1 inBetulaPollen Using Monoclonal Antibody Labelling

Grana ◽  
1996 ◽  
Vol 35 (6) ◽  
pp. 369-374 ◽  
Author(s):  
Gamal El-Ghazaly ◽  
Sumio Nakamura ◽  
Yuichi Takahashi ◽  
Mauro Cresti ◽  
Bjöorn Walles ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Major Allergen ◽  
Bet V 1 ◽  
Antibody Labelling

Quantification of the Major Allergen from Cypress (Cupressus arizonica) Pollen, Cup a 1, by Monoclonal Antibody-Based ELISA

2004 ◽  
Vol 134 (1) ◽  
pp. 10-16 ◽  
Author(s):  
M.C. Arilla ◽  
I. Ibarrola ◽  
R. García ◽  
B. de la Hoz ◽  
A. Martínez ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Major Allergen ◽  
Cupressus Arizonica
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