Isolation and characterization of rice cesium transporter genes from a rice-transporter-enriched yeast expression library

2017 ◽  
Vol 160 (4) ◽  
pp. 425-436 ◽  
Author(s):  
Tomohiro Yamaki ◽  
Masahiro Otani ◽  
Kohei Ono ◽  
Takuro Mimura ◽  
Koshiro Oda ◽  
...  
Keyword(s):  
Yeast Expression ◽  
Expression Library ◽  
Isolation And Characterization

Isolation and characterization of a putative collagen gene from the potato cyst nematode Globodera pallida

Parasitology ◽  
1996 ◽  
Vol 113 (6) ◽  
pp. 581-588 ◽  
Author(s):  
J. T. Jones ◽  
R. H. Curtis ◽  
P. J. Wightman ◽  
P. R. Burrows
Keyword(s):  
Cyst Nematode ◽  
Globodera Pallida ◽  
Collagen Gene ◽  
Expression Library ◽  
Northern Blots ◽  
Isolation And Characterization ◽  
Collagen Genes ◽  
Mixed Stage

SUMMARYA cDNA clone encoding a full length putative collagen has been isolated in a screen of a mixed stage Globodera pallida expression library. Comparison of the deduced amino acid sequence of this molecule with other collagens suggests it is a cuticular collagen and a member of the col-8 subfamily of collagen genes. Northern blots show the gene is expressed specifically in gravid, adult females of the parasite as compared to second (invasive) stage juveniles and virgin females. Preliminary immunocytochemical studies indicate this collagen is present in areas other than the cuticle; these findings and the potential functional role of this collagen are discussed.

Isolation and Characterization of Human Blood Platelet mRNA and Construction of a cDNA Library in λgt11

1989 ◽  
Vol 61 (03) ◽  
pp. 448-453 ◽  
Author(s):  
Andreas N Wicki ◽  
Alfred Walz ◽  
Susan N Gerber-Huber ◽  
Roland H Wenger ◽  
Rolf Vornhagen ◽  
...  
Keyword(s):  
Human Blood ◽  
Blood Platelets ◽  
Blood Platelet ◽  
Cdna Expression Library ◽  
Expression Library ◽  
Purification Method ◽  
Isolation And Characterization ◽  
Reticulocyte Lysate ◽  
Human Blood Platelets

SummaryWe have developed a purification method for the isolation of platelet-specific poly (A+) RNA and demonstrated that human blood platelets, despite the absence of a nucleus, contain stable mRNA. The poly (A+) RNA was used to construct a platelet- specific cDNA expression library in λgtll. The platelet derivation of the purified mRNA was confirmed by identification of membrane glycoprotein Ib (GPIb) message by immunoprecipitation of rabbit reticulocyte lysate translation products with poly- and monoclonal antibodies against GPIbα and by sequencing of a GPIbα cDNA clone

The isolation and characterization of 18 equine microsatellite loci, TKY272–TKY289

2000 ◽  
Vol 31 (2) ◽  
pp. 149-149 ◽  
Author(s):  
T Tozaki ◽  
H Kakoi ◽  
S Mashima ◽  
K Hirota ◽  
T Hasegawa ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Isolation And Characterization

Isolation and characterization of new phenanthrenes from Juncus inflexus and their chemotaxonomic significance

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
B Tóth ◽  
N Kúsz ◽  
A Csorba ◽  
T Kurtán ◽  
J Hohmann ◽  
...  
Keyword(s):  
Isolation And Characterization

Isolation and characterization of a novel O-methyltransferase from the tropical liana Triphyophyllum peltatum

2017 ◽  
Author(s):  
L Passolt ◽  
A Jindaprasert ◽  
T Le Tran ◽  
R Seupel ◽  
G Bringmann ◽  
...  
Keyword(s):  
Isolation And Characterization
Sign in / Sign up

Export Citation Format

Share Document