scholarly journals West Nile virus nucleic acid persistence in whole blood months after clearance in plasma: implication for transfusion and transplantation safety

Transfusion ◽  
2014 ◽  
Vol 54 (12) ◽  
pp. 3232-3241 ◽  
Author(s):  
Marion C. Lanteri ◽  
Tzong-Hae Lee ◽  
Li Wen ◽  
Zhanna Kaidarova ◽  
Marjorie D. Bravo ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Nucleic Acid ◽  
Whole Blood ◽  
West Nile

scholarly journals Extraordinary increase in West Nile virus cases and first confirmed human Usutu virus infection in Hungary, 2018

2019 ◽  
Vol 24 (28) ◽  
Author(s):  
Anna Nagy ◽  
Eszter Mezei ◽  
Orsolya Nagy ◽  
Tamás Bakonyi ◽  
Nikolett Csonka ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Nucleic Acid ◽  
Whole Blood ◽  
Urine Samples ◽  
Differential Diagnostics ◽  
Sample Type ◽  
West Nile ◽  
Viral Nucleic Acid ◽  
Transmission Season ◽  
Usutu Virus

Background During the 2018 WNV transmission season, similarly to other endemic areas in Europe, a large number of human West Nile virus (WNV) infections were reported in Hungary. Aims We summarise the epidemiological and laboratory findings of the 2018 transmission season and expand experiences in flavivirus differential diagnostics. Methods Every patient with clinical suspicion of acute WNV infection was in parallel tested for WNV, tick-borne encephalitis virus and Usutu virus (USUV) by serological methods. Sera, whole blood and urine samples were also tested for the presence of viral nucleic acid. Results Until the end of December 2018, 215 locally acquired and 10 imported human WNV infections were notified in Hungary. All reported cases were symptomatic; most of them exhibited neurological symptoms. In a large proportion of tested individuals, whole blood was the most appropriate sample type for viral nucleic acid detection, but because whole blood samples were not always available, testing of urine samples also extended diagnostic possibilities. In addition, the first human USUV infection was confirmed in 2018 in a patient with aseptic meningitis. Serological cross-reactions with WNV in different serological assays were experienced, but subsequent molecular biological testing and sequence analysis identified Europe lineage 2 USUV infection. Conclusion Careful interpretation and simultaneous application of different laboratory methods are necessary to avoid misdiagnosis of human USUV cases. Expansion of the laboratory-confirmed case definition criteria for detection of viral RNA in any clinical specimens to include urine samples could increase diagnostic sensitivity.

scholarly journals Detection of West Nile virus-specific antibodies and nucleic acid in horses and mosquitoes, respectively, in Nuevo Leon State, northern Mexico, 2006-2007

2012 ◽  
Vol 26 (3) ◽  
pp. 351-354 ◽  
Author(s):  
L. IBARRA-JUAREZ ◽  
L. EISEN ◽  
B. G. BOLLING ◽  
B. J. BEATY ◽  
B. J. BLITVICH ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Nucleic Acid ◽  
West Nile ◽  
Nuevo Leon ◽  
Specific Antibodies ◽  
Northern Mexico ◽  
Nuevo León

The Informed Consent Process in Whole Blood Donation

2008 ◽  
Vol 132 (6) ◽  
pp. 947-951 ◽  
Author(s):  
Lindsay A. Alaishuski ◽  
Rodney D. Grim ◽  
Ronald E. Domen
Keyword(s):  
West Nile Virus ◽  
Informed Consent ◽  
Whole Blood ◽  
Blood Donors ◽  
Blood Donation ◽  
Informed Consent Process ◽  
Positive Test ◽  
Consent Process ◽  
West Nile ◽  
Eligibility Requirements

Abstract Context.—Informed consent in transfusion medicine has been an area lacking of significant research and it is unknown if donors fully comprehend the risks associated with whole blood donation. Objective.—To assess the adequacy of the informed consent process in whole blood donation. Design.—A brief questionnaire was constructed and distributed to whole blood donors visiting various fixed and mobile donor sites of the Central Pennsylvania Blood Bank. Questions consisted of demographic information; donor opinions of information content, length, and comprehension; and a short quiz pertaining to donor risks and eligibility. Results.—Analysis of 849 surveys demonstrated that donors comprehended a mean of 73.5% of the various donor eligibility and risks that were surveyed. Female and younger donors scored statistically higher on comprehension questions compared with male and older counterparts. Donors were most aware of (1) donor eligibility requirements related to acquired immunodeficiency syndrome comprehension, (2) the risk of dizziness postdonation, and (3) having lived in a certain country (93.7%–95.6% comprehension, respectively). Donors were least aware of (1) the risk of a possible referral to a physician for outstanding medical conditions or positive test results, (2) the risk of a positive test result, and (3) West Nile virus testing information (22.4%–49.3% comprehension, respectively). Conclusions.—Whole blood donors believed that they were giving informed consent, but a significant percentage of donors were unaware of several of the risks associated with blood donation, including participation in West Nile virus research testing. Our data suggest that donors do not fully comprehend the risks of whole blood donation and that repetition of information to the donor, and in multiple formats, strengthens the level of comprehension and thus the informed consent process.

scholarly journals Screening of Organ and Tissue Donors for West Nile Virus by Nucleic Acid Amplification - A Three Year Experience in Alberta

2008 ◽  
Vol 8 (10) ◽  
pp. 2119-2125 ◽  
Author(s):  
Peter A. G. Tilley ◽  
Julie D. Fox ◽  
Bonita Lee ◽  
Linda Chui ◽  
Jutta Preiksaitis
Keyword(s):  
West Nile Virus ◽  
Nucleic Acid ◽  
Nucleic Acid Amplification ◽  
West Nile ◽  
Tissue Donors

Probable transfusion transmission of West Nile virus from an apheresis platelet that screened non‐reactive by individual donor‐nucleic acid testing

Transfusion ◽  
2019 ◽  
Vol 60 (2) ◽  
pp. 424-429 ◽  
Author(s):  
Chelsea Hayes ◽  
Laura Stephens ◽  
Joy L. Fridey ◽  
Robert E. Snyder ◽  
Jamel A. Groves ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Nucleic Acid ◽  
Nucleic Acid Testing ◽  
West Nile ◽  
Individual Donor

West Nile virus testing experience in 2007: evaluation of different criteria for triggering individual-donation nucleic acid testing

Transfusion ◽  
2009 ◽  
Vol 49 (6) ◽  
pp. 1160-1170 ◽  
Author(s):  
Steven H. Kleinman ◽  
Joan Dunn Williams ◽  
Gene Robertson ◽  
Sally Caglioti ◽  
Robert C. Williams ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Nucleic Acid ◽  
Nucleic Acid Testing ◽  
West Nile ◽  
Virus Testing

Associations between West Nile virus infection and symptoms reported by blood donors identified through nucleic acid test screening

Transfusion ◽  
2009 ◽  
Vol 49 (2) ◽  
pp. 278-288 ◽  
Author(s):  
Brian Custer ◽  
Hany Kamel ◽  
Nancy E. Kiely ◽  
Edward L. Murphy ◽  
Michael P. Busch
Keyword(s):  
West Nile Virus ◽  
Nucleic Acid ◽  
Virus Infection ◽  
Blood Donors ◽  
West Nile Virus Infection ◽  
West Nile ◽  
Nucleic Acid Test ◽  
Acid Test
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