scholarly journals Splitting of a Prevalent Mycobacterium bovis Spoligotype by Variable-Number Tandem-Repeat Typing Reveals High Heterogeneity in an Evolving Clonal Group

2013 ◽  
Vol 51 (11) ◽  
pp. 3658-3665 ◽  
Author(s):  
Sabrina Rodriguez-Campos ◽  
Yurena Navarro ◽  
Beatriz Romero ◽  
Lucía de Juan ◽  
Javier Bezos ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Tandem Repeat ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Clonal Group

scholarly journals Genotyping North American Animal Mycobacterium Bovis Isolates Using Multilocus Variable Number Tandem Repeat Analysis

2008 ◽  
Vol 20 (6) ◽  
pp. 707-715 ◽  
Author(s):  
Lorene R. Martinez ◽  
Beth Harris ◽  
William C. Black ◽  
Robert M. Meyer ◽  
Patrick J. Brennan ◽  
...  
Keyword(s):  
North America ◽  
Mycobacterium Bovis ◽  
Tandem Repeat ◽  
North American ◽  
Variable Number Tandem Repeat ◽  
Epidemiological Data ◽  
Variable Number ◽  
Repeat Analysis ◽  
Trace Back ◽  
Animal Isolates

Multilocus variable number tandem repeat analysis (MLVA) has recently emerged as a genotyping method that is both robust and highly discriminatory for the differentiation of Mycobacterium tuberculosis complex (MTBC) strains, including Mycobacterium bovis. However, MLVA assessment of M. bovis isolates recovered from animals in North America has been limited. Using an epidemiologically diverse set of 41 North American M. bovis animal isolates, MLVA, based on 27 published variable number tandem repeat (VNTR) loci, was evaluated. Nineteen loci displayed polymorphism, which resulted in differentiation of 21 unique MLVA genotypes. A subset of 6 loci differentiated the isolates into 14 genetically related groups that displayed remarkable concordance with the epidemiological data gathered via traditional trace-back methods. In most cases, MLVA exhibited greater resolution than spoligotyping, which differentiated the isolates into 11 groups. MLVA genotyping of M. bovis shows great potential as a molecular typing tool for characterizing the epidemiology of M. bovis animal infections in North America. However, the greatest resolution was achieved by using a combination of both MLVA and spoligotyping.

scholarly journals Spoligotyping and variable number tandem repeat analysis of Mycobacterium bovis isolates from cattle in Brazil

2012 ◽  
Vol 107 (1) ◽  
pp. 64-73 ◽  
Author(s):  
Patrícia Martins Parreiras ◽  
Giovanna Ivo Andrade ◽  
Telma de Figueiredo do Nascimento ◽  
Maraníbia Cardoso Oelemann ◽  
Harrison Magdinier Gomes ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Tandem Repeat ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Repeat Analysis

Evaluation of the discriminatory power of variable number tandem repeat (VNTR) typing of Mycobacterium bovis strains

2005 ◽  
Vol 109 (3-4) ◽  
pp. 217-222 ◽  
Author(s):  
Markus Hilty ◽  
Colette Diguimbaye ◽  
Esther Schelling ◽  
Franca Baggi ◽  
Marcel Tanner ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Tandem Repeat ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
Discriminatory Power

scholarly journals Limitations of Spoligotyping and Variable-Number Tandem-Repeat Typing for Molecular Tracing of Mycobacterium bovis in a High-Diversity Setting

2011 ◽  
Vol 49 (9) ◽  
pp. 3361-3364 ◽  
Author(s):  
Sabrina Rodriguez-Campos ◽  
Alicia Aranaz ◽  
Lucía de Juan ◽  
José Luis Sáez-Llorente ◽  
Beatriz Romero ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Tandem Repeat ◽  
Variable Number Tandem Repeat ◽  
Variable Number ◽  
High Diversity
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