False-Positive Reactions in the Latex Agglutination Test for Cryptococcus neoformans Antigen

1991 ◽  
Vol 29 (9) ◽  
pp. 2091-2091
Keyword(s):  
Cryptococcus Neoformans ◽  
False Positive ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test ◽  
False Positive Reactions

scholarly journals False positive reactions with rotavirus latex agglutination test.

1992 ◽  
Vol 45 (5) ◽  
pp. 460-460 ◽  
Author(s):  
N Brink ◽  
S Rice ◽  
C Pickering ◽  
S Thurlbeck
Keyword(s):  
False Positive ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test ◽  
False Positive Reactions

Frequent false-positive results ofAspergillus latex agglutination test

Cancer ◽  
1999 ◽  
Vol 86 (2) ◽  
pp. 274-281 ◽  
Author(s):  
Masahiro Kami ◽  
Yoshinobu Kanda ◽  
Seishi Ogawa ◽  
Shin-ichiro Mori ◽  
Yuji Tanaka ◽  
...  
Keyword(s):  
False Positive ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test ◽  
Positive Results

Development of a Latex Agglutination Test for the Rapid Identification of Vibrio parahaemolyticus

1994 ◽  
Vol 57 (1) ◽  
pp. 31-36 ◽  
Author(s):  
TSUNG C. CHANG ◽  
CHI H. CHEN ◽  
HUI C. CHEN
Keyword(s):  
Outer Membrane ◽  
False Positive ◽  
Vibrio Parahaemolyticus ◽  
Outer Membrane Proteins ◽  
False Negative ◽  
Sodium Dodecyl ◽  
Rapid Identification ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Latex Agglutination Test

A latex agglutination test for the rapid identification of Vibrio parahaemolyticus has been developed. Two bacterial outer membrane proteins, with molecular weights of 36,000 and 34,000, were obtained by preparative sodium dodecyl sulfate polyacrylamide gel electrophoresis and were subsequently used as antigens for producing antibodies in rabbits. Immunoelectron microscopy demonstrated that the two proteins were present in the outer membrane of the microorganism. Latex particles sensitized with the affinity-purified antibodies were used as a reagent for the rapid identification of the bacterium. Of 173 strains (including 94 isolates of V. parahaemolyticus, 40 isolates of other vibrios and 39 strains of other bacteria) tested, the false-negative and false-positive rates were 1.4 and 3.1%, respectively. Some strains of three vibrios (V. alginolyticus, V. harveyi, and V. mimicus) produced false-positive results. However, no cross-reactions were observed with the latex reagent among the 39 strains (33 species) of other bacteria. It is proposed that suspicious colonies (green or blue) of V. parahaemolyticus on thiosulfate-citrate-bile salts-sucrose agar (TCBS) be subcultured to tryptic soy agar with 3% NaCl for overnight incubation. Cultures grown on tryptic soyagar-3% NaCl may be used for latex agglutination and galactosidase assays. V. parahaemolyticus will be latex positive and galactosidase negative. Under this condition, V. parahaemolyticus can be identified in only one day after suspect colonies were observed on TCBS agar.

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