Generation and characterization of monoclonal antibodies to the putative CD4-binding domain of human immunodeficiency virus type 1 gp120.

1989 ◽  
Vol 63 (9) ◽  
pp. 3579-3585 ◽  
Author(s):  
N C Sun ◽  
D D Ho ◽  
C R Sun ◽  
R S Liou ◽  
W Gordon ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Monoclonal Antibodies ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Binding Domain ◽  
Immunodeficiency Virus

scholarly journals Monoclonal Antibodies against the Minimal DNA-Binding Domain in the Carboxyl-Terminal Region of Human Immunodeficiency Virus Type 1 Integrase

1999 ◽  
Vol 73 (5) ◽  
pp. 4475-4480 ◽  
Author(s):  
Tetsuya Ishikawa ◽  
Nobuo Okui ◽  
Noriko Kobayashi ◽  
Ryuta Sakuma ◽  
Tadaichi Kitamura ◽  
...  
Keyword(s):  
Amino Acids ◽  
Human Immunodeficiency Virus ◽  
Monoclonal Antibodies ◽  
Dna Binding ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Binding Domain ◽  
Dna Binding Domain ◽  
Immunodeficiency Virus

ABSTRACT Integrase of human immunodeficiency virus type 1 (HIVIN) consists of 288 amino acids, and its minimum DNA-binding domain (MDBD) (amino acids [aa] 220 to 270) is required for the integration reaction. We produced and characterized four murine monoclonal antibodies (MAbs) to the MDBD of HIVIN (strain LAI). Immunoblot and enzyme-linked immunosorbent assays with truncated HIVINs showed that those MAbs recognized sequential epitopes within the MDBD (aa 228 to 236, 237 to 252, 253 to 261, and 262 to 270). Their binding to HIVIN inhibited terminal cleavage and strand transfer activities but not disintegration activity in vitro. This collection of MAbs is useful for studying the structure and function of the MDBD by complementing mutational analyses and other biochemical studies.

Genotypic Characterization of Human Immunodeficiency Virus Type 1 Derived from Antiretroviral Therapy-Naive Individuals Residing in Sorong, West Papua

2016 ◽  
Vol 32 (8) ◽  
pp. 812-817 ◽  
Author(s):  
Adiana Mutamsari Witaningrum ◽  
Tomohiro Kotaki ◽  
Siti Qamariyah Khairunisa ◽  
Muhammad Qushai Yunifiar M ◽  
Dwi Wahyu Indriati ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Antiretroviral Therapy ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Genotypic Characterization ◽  
Immunodeficiency Virus ◽  
West Papua
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