Binding of Intracellular Anti-Rev Single Chain Variable Fragments to Different Epitopes of Human Immunodeficiency Virus Type 1 Rev: Variations in Viral Inhibition

2001 ◽  
Vol 75 (2) ◽  
pp. 1092-1092
Author(s):  
Yong Wu ◽  
Lingxun Duan ◽  
Minghua Zhu ◽  
Baocheng Hu ◽  
Satoshi Kubota ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Viral Inhibition ◽  
Single Chain ◽  
Immunodeficiency Virus ◽  
Single Chain Variable Fragments

scholarly journals Production of Uninfectious Human Immunodeficiency Virus Type 1 Containing Viral Protein R Fused to a Single-Chain Antibody against Viral Integrase

1998 ◽  
Vol 72 (8) ◽  
pp. 6960-6964 ◽  
Author(s):  
Nobuo Okui ◽  
Noriko Kobayashi ◽  
Yoshihiro Kitamura
Keyword(s):  
Human Immunodeficiency Virus ◽  
Fusion Protein ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Viral Protein ◽  
Single Chain ◽  
Single Chain Antibody ◽  
Immunodeficiency Virus ◽  
Hiv 1

ABSTRACT A single-chain antibody (scAb) against human immunodeficiency virus type 1 (HIV-1) integrase was expressed as a fusion protein of scAb and HIV-1 viral protein R (Vpr), together with the HIV-1 genome, in human 293T cells. The expression did not affect virion production much but markedly reduced the infectivity of progeny virions. The fusion protein was found to be incorporated into the virions. The incorporation appears to account for the reduced infectivity.

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