Amino acid sequence of cyanogen bromide fragment CB5 of human hemopexin

Cyanogen bromide fragment CB5 represents the region of the polypeptide chain of hemopexin between the fourth and fifth methionine residue (residues 232-352). It contains 120 amino acid residues in the following sequence: Arg-Cys-Ser-Pro-His-Leu-Val-Leu-Ser-Ala-Leu-Thr-Ser-Asp-Asn-His-Gly-Ala-Thr-Tyr-Ala-Phe-Ser-Gly-Thr-His-Tyr-Trp-Arg-Leu-Asp-Thr-Ser-Arg-Asp-Gly-Trp-His-Ser-Trp-Pro-Ile-Ala-His-Gln-Trp-Pro-Gln-Gly-Pro-Ser-Ala-Val-Asp-Ala-Ala-Phe-Ser-Trp-Glu-Glu-Lys-Leu-Tyr-Leu-Val-Gln-Gly-Thr-Gln-Val-Tyr-Val-Phe-Leu-Thr-Lys-Gly-Gly-Tyr-Thr-Leu-Val-Ser-Gly-Tyr-Pro-Lys-Arg-Leu-Glu-Lys-Glu-Val-Gly-Thr-Pro-His-Gly-Ile-Ile-Leu-Asp-Ser-Val-Asp-Ala-Ala-Phe-Ile-Cys-Pro-Gly-Ser-Ser-Arg-Leu-His-Ile-Met. The sequence was derived from the data on peptides prepared by cleavage of fragment CB5 by mild acid hydrolysis, by trypsin and chymotrypsin.

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Thermitase from Thermoactinomyces vulgaris; amino acid sequence of the large N-terminal cyanogen bromide peptide

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19850885 ◽

1985 ◽

Vol 50 (4) ◽

pp. 885-896 ◽

Cited By ~ 4

Author(s):

Bedřich Meloun ◽

Miroslav Baudyš ◽

Manfred Pavlík ◽

Vladimír Kostka ◽

Gert Hausdorf ◽

...

Keyword(s):

Amino Acid ◽

Sequence Analysis ◽

Amino Acid Sequence ◽

High Performance ◽

Cyanogen Bromide ◽

Sequential Data ◽

Amino Acid Residues ◽

Methionine Residue ◽

Large N ◽

Thermoactinomyces Vulgaris

The large cyanogen bromide fragment (CB1) represents the N-terminal part of the molecule of thermitase and contains 226 amino acid residues. Its molecular weight calculated from sequential data is 22 932 (the C-terminal residue is regarded as a methionine residue in the calculations). The amino acid sequence of fragment CB1 was determined by analysis of peptides obtained by tryptic hydrolysis of the fragment; these data were complemented by sequence analysis of the chymotryptic digest of fragment Mf (residues 75 through 226) and of chymotryptic fragment ET3 (residues 103 through 226) isolated from the limited tryptic digest of fragment CB1. The peptides were purified by high performance liquid chromatography and by thin layer techniques. The sequence analysis of the large peptides was effected in the sequenator, small peptides were sequenced manually by the DABITC/PITC double coupling technique. The results obtained in this study together with those of previous work5 permitted the complete amino acid sequence of fragment CB1 to be determined.

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Amino Acid Sequence of Cyanogen Bromide Fragment CN2 From a Hong Kong Influenza Haemagglutinin Heavy Chain

Australian Journal of Biological Sciences ◽

10.1071/bi9800137 ◽

1980 ◽

Vol 33 (2) ◽

pp. 137 ◽

Cited By ~ 2

Author(s):

Colin W Ward ◽

Theo AA Dopheide ◽

Adam S Inglis

Keyword(s):

Hong Kong ◽

Amino Acid ◽

Amino Acid Sequence ◽

Heavy Chain ◽

Cyanogen Bromide ◽

Edman Degradation ◽

Amino Acid Residues ◽

Core Peptide ◽

Cyanogen Bromide Fragment

The amino acid sequence of cyanogen bromide peptide CN2 from the heavy chain (HAl) of the haemagglutinin of the Hong Kong variant A/Memphis/l02/72 has been obtained by direct, automated sequence analysis on the whole fragment and by manual dansyl-Edman degradation of tryptic, peptic and chymotryptic peptides. It was found to contain 92 amino acid residues, including a large, insoluble, tryptic core peptide (residues 62-87). It did not contain any half-cystine residues or carbohydrate. The determination of its structure was complicated by the presence of an Asn-Ile bond at positions 48-49 which was readily cleaved by both trypsin and chymotrypsin.

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Amino acid sequence of cyanogen bromide fragment CB5 of hog pepsin

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19780344 ◽

1978 ◽

Vol 43 (1) ◽

pp. 344-355

Author(s):

Ladislav Morávek

Keyword(s):

Amino Acid ◽

Amino Acid Sequence ◽

Cyanogen Bromide ◽

Cyanogen Bromide Fragment

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Amino-acid sequence of cyanogen bromide fragment CB5(Phe) of human plasma albumin

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19753932 ◽

1975 ◽

Vol 40 (12) ◽

pp. 3932-3941 ◽

Cited By ~ 2

Author(s):

L. Morávek ◽

V. Kostka ◽

I. Rosenberg ◽

B. Meloun

Keyword(s):

Amino Acid ◽

Amino Acid Sequence ◽

Human Plasma ◽

Cyanogen Bromide ◽

Plasma Albumin ◽

Cyanogen Bromide Fragment

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Amino acid sequence of cyanogen bromide fragment CB3 of hog pepsin

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19810655 ◽

1981 ◽

Vol 46 (3) ◽

pp. 655-666

Author(s):

Ladislav Morávek ◽

Vladimír Kostka

Keyword(s):

Amino Acid ◽

Amino Acid Sequence ◽

Cyanogen Bromide ◽

Methionine Residues ◽

Cyanogen Bromide Fragment

On the basis of the knowlidge of thermolytic, chymotryptic and substilisin peptides the amino acid sequence was determined of cyanogen bromide fragment CB3 representing the region between methionine residues I and II of pepsin: Thr-Gly-Ile-Leu-Gly-Tyr-Asp-Thr-Val-Gln-Val-Gly-Gly-Ile-Ser-Asp-Thr-Asn-Gln-Ile-Phe-Gly-Leu-Ser-Glu-Thr-Glu-Pro-Gly-Ser-Phe-Leu-Tyr-Tyr-Ala-Pro-Phe-Asp-Gly-Ile-Leu-Gly-Leu-Ala-Tyr-Pro-Ser-Ile-Ser-Ala-Ser-Gly-Ala-Thr-Pro-Val-Phe-Asp-Asn-Leu-Trp-Asp-Gln-Gly-Leu-Val-Ser-Gln-Asp-Leu-Phe-Ser-Val-Tyr-Leu-Ser-Ser-Asn-Asp-Asp-Ser-Gly-Ser-Val-Val-Leu-Leu-Gly-Gly-Ile-Asp-Ser-Ser-Tyr-Tyr-Thr-Gly-Ser-Leu-Asn-Trp-Val-Pro-Val-Ser-Val-Glu-Gly-Tyr-Trp-Gln-Ile-Thr-Leu-Asp-Ser-Ile-Thr-Met.

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