Transport of glucose and leucine by intestinal membrane vesicles in genetic diabetes

1980 ◽  
Vol 238 (5) ◽  
pp. G419-G423 ◽  
Author(s):  
R. Bennetts ◽  
K. Ramaswamy
Keyword(s):  
Brush Border ◽  
Brush Border Membrane ◽  
Membrane Vesicles ◽  
Diabetic Mice ◽  
Drug Induced ◽  
Intestinal Brush Border Membrane ◽  
Intestinal Brush Border ◽  
Intestinal Membrane ◽  
Small Intestinal ◽  
Genetically Diabetic Mice

Na+-dependent D-glucose and L-leucine uptakes by isolated small intestinal brush-border membrane vesicles were studied in normal and genetically diabetic mice (C57BL/KsJ-dbm). Vesicles from normal mice demonstrated transport characteristics and morphological appearances identical to those from other mammalian small intestinal brush-border membrane isolates. There was no difference found between genetically diabetic mice and their littermate controls. These data suggest that the small intestinal brush-border membrane transport is not altered in genetic diabetes in contrast to that found in drug-induced diabetes.

scholarly journals Characterisation of penicillin G uptake in human small intestinal brush border membrane vesicles

Gut ◽  
1999 ◽  
Vol 44 (5) ◽  
pp. 620-624 ◽  
Author(s):  
J F Poschet ◽  
S M Hammond ◽  
P D Fairclough
Keyword(s):  
Brush Border ◽  
Brush Border Membrane ◽  
Membrane Vesicles ◽  
Brush Border Membrane Vesicles ◽  
Penicillin G ◽  
Uptake System ◽  
Intestinal Brush Border Membrane ◽  
Intestinal Brush Border ◽  
Model Molecule ◽  
Small Intestinal

BACKGROUNDMany β lactams are well absorbed by the small intestine, although the reasons for this are poorly understood.AIMSTo characterise the uptake of penicillin G into human small intestinal brush border membrane vesicles (BBMV) and to compare the uptake characteristics to those of rabbit BBMV.METHODS AND RESULTSUptake of penicillin G was studied in human BBMV. Penicillin G was actively transported into the lumen of BBMV via an H+ dependent, Na+ independent uptake system. The carrier mediated process was saturable and adhered to Michaelis-Menten kinetics (Vmax 52 nmol penicillin G per mg protein per 30 seconds,Km 13.9 mM). These results are similar to those previously reported in rabbit BBMV.CONCLUSIONSIt is suggested that penicillin G can be used as a model molecule for peptide and β lactam transport studies as it is cheap and readily available in isotopically labelled form. Furthermore, rabbit BBMV may be used as an acceptable substitute for human BBMV for the study of penicillin transport.

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