A Method for Studying the Dynamics of Transcapillary Transfer Quantitatively At the Microscopic Level in Situ in Living Organs

Angiology ◽  
1963 ◽  
Vol 14 (3) ◽  
pp. 97-106 ◽  
Author(s):  
Edward H. Bloch
Keyword(s):  
Author(s):  
Vivian V. Yang ◽  
S. Phyllis Stearner

The heart is generally considered a radioresistant organ, and has received relatively little study after total-body irradiation with doses below the acutely lethal range. Some late damage in the irradiated heart has been described at the light microscopic level. However, since the dimensions of many important structures of the blood vessel wall are submicroscopic, investigators have turned to the electron microscope for adequate visualization of histopathological changes. Our studies are designed to evaluate ultrastructural changes in the mouse heart, particularly in the capillaries and muscle fibers, for 18 months after total-body exposure, and to compare the effects of 240 rad fission neutrons and 788 rad 60Co γ-rays.Three animals from each irradiated group and three control mice were sacrificed by ether inhalation at 4 days, and at 1, 3, 6, 12, and 18 months after irradiation. The thorax was opened and the heart was fixed briefly in situwith Karnofsky's fixative.


1993 ◽  
Vol 68 (3) ◽  
pp. 169-174 ◽  
Author(s):  
Chong-Hua Yao ◽  
Sohei Kitazawa ◽  
Takahiro Fujimori ◽  
Sakan Maeda

1956 ◽  
Vol 34 (5) ◽  
pp. 869-881 ◽  
Author(s):  
Par Marc Crevier ◽  
Léonard-F. Bélanger

The cholinesterase activity of the motor end plate has been determined in situ by histophotometry. It has been possible to establish statistically the probable value of the enzymic activity of a single motor end plate morphologically identified. Studying the rate of the enzymic reaction in function of time, temperature, and concentration of substrate, it became evident that the progressive deposition of the precipitate as the reaction proceeds is represented by a proportional and local increase in its concentration and not by its spreading over the fiber. Inhibition experiments with eserine and acetylcholine in excess have proved that with the substrates used, β-naphthylacetate and β-carbonaphthoxycholine, it is possible to identify independently acetylcholinesterase and cholinesterase activity at the level of the motor end plate, the former being in proportion of 90%. The acetylcholinesterase inactivation has been observed after intramuscular injections of eserine, indicating that pharmacological problems can be studied at the microscopic level by histophotometry.


Soft Matter ◽  
2018 ◽  
Vol 14 (11) ◽  
pp. 2059-2067 ◽  
Author(s):  
Na Qi ◽  
Haoyang Sun ◽  
Hui Zhao ◽  
Ying Li

The smart foaming control performance of a swollen micelle system and the mechanism at the microscopic level were studied.


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