A Survey of Amino Acid Analysers Readily Available in the United Kingdom

Recently, a new SARS-CoV-2 lineage called B.1.1.7 (variant of concern: VOC 202012/01) emerged in the United Kingdom that was reported to spread more efficiently and faster than other strains. This variant has an unusually large number of mutations with 10 amino acid changes in the spike protein, raising concerns that its recognition by neutralizing antibodies may be affected. Here, we tested SARS-CoV-2-S pseudoviruses bearing either the Wuhan reference strain or the B.1.1.7 lineage spike protein with sera of 40 participants who were vaccinated in a previously reported trial with the mRNA-based COVID-19 vaccine BNT162b2. The immune sera had slightly reduced but overall largely preserved neutralizing titers against the B.1.1.7 lineage pseudovirus. These data indicate that the B.1.1.7 lineage will not escape BNT162b2-mediated protection.

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Neutralization of SARS-CoV-2 lineage B.1.1.7 pseudovirus by BNT162b2 vaccine-elicited human sera

10.1101/2021.01.18.426984 ◽

2021 ◽

Cited By ~ 8

Author(s):

Alexander Muik ◽

Ann-Kathrin Wallisch ◽

Bianca Sänger ◽

Kena A. Swanson ◽

Julia Mühl ◽

...

Keyword(s):

United Kingdom ◽

Amino Acid ◽

Neutralizing Antibodies ◽

Reference Strain ◽

Spike Protein ◽

The United Kingdom ◽

Human Sera

AbstractRecently, a new SARS-CoV-2 lineage called B.1.1.7 has emerged in the United Kingdom that was reported to spread more efficiently than other strains. This variant has an unusually large number of mutations with 10 amino acid changes in the spike protein, raising concerns that its recognition by neutralizing antibodies may be affected. Here, we investigated SARS-CoV-2-S pseudoviruses bearing either the Wuhan reference strain or the B.1.1.7 lineage spike protein with sera of 16 participants in a previously reported trial with the mRNA-based COVID-19 vaccine BNT162b2. The immune sera had equivalent neutralizing titers to both variants. These data, together with the combined immunity involving humoral and cellular effectors induced by this vaccine, make it unlikely that the B.1.1.7 lineage will escape BNT162b2-mediated protection.

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Early empirical assessment of the N501Y mutant strains of SARS-CoV-2 in the United Kingdom, October to November 2020

10.1101/2020.12.20.20248581 ◽

2020 ◽

Author(s):

Kathy Leung ◽

Marcus HH Shum ◽

Gabriel M Leung ◽

Tommy TY Lam ◽

Joseph T Wu

Keyword(s):

United Kingdom ◽

Amino Acid ◽

Receptor Binding ◽

Binding Domain ◽

Spike Protein ◽

Receptor Binding Domain ◽

Amino Acid Deletion ◽

The United Kingdom ◽

Mutant Strains ◽

The Uk

AbstractTwo new SARS-CoV-2 lineages with the N501Y mutation in the receptor binding domain of the spike protein have rapidly become prevalent in the UK. We estimated that the earlier 501Y lineage without amino acid deletion Δ69/Δ70 circulating mainly between early September to mid-November was 10% (6-13%) more transmissible than the 501N lineage, and the currently dominant 501Y lineage with amino acid deletion Δ69/Δ70 circulating since late September was 75% (70-80%) more transmissible than the 501N lineage.

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Early transmissibility assessment of the N501Y mutant strains of SARS-CoV-2 in the United Kingdom, October to November 2020

Eurosurveillance ◽

10.2807/1560-7917.es.2020.26.1.2002106 ◽

2021 ◽

Vol 26 (1) ◽

Author(s):

Kathy Leung ◽

Marcus HH Shum ◽

Gabriel M Leung ◽

Tommy TY Lam ◽

Joseph T Wu

Keyword(s):

United Kingdom ◽

Amino Acid ◽

Receptor Binding ◽

Binding Domain ◽

Spike Protein ◽

Receptor Binding Domain ◽

Amino Acid Deletion ◽

The United Kingdom ◽

Mutant Strains

Two new SARS-CoV-2 lineages with the N501Y mutation in the receptor-binding domain of the spike protein spread rapidly in the United Kingdom. We estimated that the earlier 501Y lineage without amino acid deletion Δ69/Δ70, circulating mainly between early September and mid-November, was 10% (6–13%) more transmissible than the 501N lineage, and the 501Y lineage with amino acid deletion Δ69/Δ70, circulating since late September, was 75% (70–80%) more transmissible than the 501N lineage.

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