The paper aims to suggest possibilities of using molecular biology in environmental biomonitoring and importance of the linearity of quantification method applied to the DNA from the honeybee parasite Varroa destructor.
The laboratory work was carried out was in the Livestock Genotypization Laboratory of the Faculty of Animal Science and Biotechnology, University of Agricultural Sciences and Veterinary Medicine Cluj – Napoca.
According to the methodology proposed by Anderson (2000), the DNA extraction was performed using the standard methodology reagents, but their doses were modified for obtaining the best extraction yield.
The resulted linearity equation was y = 0.0675 + 0.0009x, demonstrating the validity of our initial assumption. The correlation coefficient between quantitative DNA determination and absorption (r = 0.9459) was very strong with very significant differences (P < 0.001).
Research must be continued in order to perform a validation portfolio for the honeybee parasite Varroa destructor by calculating the other needed parameters, together with implementation of the revised standard 17043, the Vocabulary of Basic and General Terms in Metrology and Guide for Expression of Uncertainty in Measurements.
Tools for Molecular Environmental Biomonitoring - Testing Linearity of the DNA Extraction Method In Varroa Destructor Mite
