Affinity Purification of Monoclonal Antibodies

2003 ◽  
pp. 49-56
Author(s):  
Alexander Schwarz
Keyword(s):  
Monoclonal Antibodies ◽  
Affinity Purification

[24] Affinity purification of oligosaccharides using monoclonal antibodies

1987 ◽  
pp. 307-313 ◽  
Author(s):  
D. Zopf ◽  
K. Schroer ◽  
G. Hansson ◽  
J. Dakour ◽  
A. Lundblad
Keyword(s):  
Monoclonal Antibodies ◽  
Affinity Purification

Engineering Staphylococcal Protein A for high-throughput affinity purification of monoclonal antibodies

2020 ◽  
Vol 44 ◽  
pp. 107632
Author(s):  
Vinod Amritkar ◽  
Satish Adat ◽  
Vijay Tejwani ◽  
Anurag Rathore ◽  
Rahul Bhambure
Keyword(s):  
Monoclonal Antibodies ◽  
High Throughput ◽  
Affinity Purification ◽  
Protein A ◽  
Staphylococcal Protein A ◽  
Staphylococcal Protein

Characterization of Monoclonal Antibodies Recognizing Human Merosin and Their Use in Affinity Purification of Native Merosin

Hybridoma ◽  
1996 ◽  
Vol 15 (5) ◽  
pp. 373-378 ◽  
Author(s):  
TOSHIHIKO MIZUTA ◽  
HISAKI SHIMADA ◽  
KATSUHIKO ARAI ◽  
HISAE HORI ◽  
SHUNJI HATTORI ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Affinity Purification

Monoclonal Antibodies to the Soluble Human IL-6 Receptor: Affinity Purification, ELISA, and Inhibition of Ligand Binding

Hybridoma ◽  
1991 ◽  
Vol 10 (1) ◽  
pp. 137-146 ◽  
Author(s):  
DANIELA NOVICK ◽  
HARTMUT ENGELMANN ◽  
M. REVEL ◽  
O. LEITNER ◽  
MENACHEM RUBINSTEIN
Keyword(s):  
Monoclonal Antibodies ◽  
Ligand Binding ◽  
Affinity Purification ◽  
Receptor Affinity

Monoclonal antibodies to bovine and human acrosin

1986 ◽  
Vol 64 (12) ◽  
pp. 1242-1248 ◽  
Author(s):  
John S. Elce ◽  
Elise J. Graham ◽  
Gizela Zboril ◽  
Lisette Leyton ◽  
Elcira Perez ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Ethyl Ester ◽  
Indirect Immunofluorescence ◽  
Zona Pellucida ◽  
Affinity Purification ◽  
Human Sperm ◽  
Cross Reaction ◽  
Sperm Cells ◽  
Immunosorbent Assays ◽  
Hydrolysis Of

Monoclonal antibodies to human acrosin were required for studies of immunological interference with fertilization. Since human acrosin was not available in adequate amounts, monoclonal antibodies have been raised in mice against purified bovine acrosin and screened for cross-reaction with human sperm cells. Two of these antibodies are described, B4F6 and C2E5. Data from enzyme-linked immunosorbent assays, immunoblots, immunoprecipitation, and indirect immunofluorescence on sperm cells indicate that B4F6 binds only to bovine acrosin, and that C2E5 binds both to bovine and to human acrosin at a conformationally determined epitope. The antibodies do not inhibit the hydrolysis of benzoylarginine ethyl ester by acrosin, but C2E5 did inhibit the dissolution of the hamster zona pellucida by purified human acrosin. The antibodies have also been used for affinity purification of acrosin and proacrosin.

Affinity Depletion and Affinity Purification of Human Factor IX by Monoclonal Antibodies

1983 ◽  
pp. 403-407 ◽  
Author(s):  
A.H. GOODALL ◽  
D.P. O'BRIEN ◽  
E. RAWLINGS ◽  
F. ROTBLAT ◽  
E.G.D. TUDDENHAM
Keyword(s):  
Monoclonal Antibodies ◽  
Human Factor ◽  
Affinity Purification ◽  
Factor Ix ◽  
Human Factor Ix

Monoclonal Antibodies to Murine Interferon-γ: Affinity Purification and Molecular Characterization of Murine Interferon-γ

1985 ◽  
Vol 5 (1) ◽  
pp. 199-208 ◽  
Author(s):  
GIORGIO GRIBAUDO ◽  
FRANCA COFANO ◽  
MARIA PRAT ◽  
SANTO LANDOLFO
Keyword(s):  
Monoclonal Antibodies ◽  
Molecular Characterization ◽  
Affinity Purification ◽  
Interferon Γ
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