Monoclonal Antibodies to Murine Interferon-γ: Affinity Purification and Molecular Characterization of Murine Interferon-γ

1985 ◽  
Vol 5 (1) ◽  
pp. 199-208 ◽  
Author(s):  
GIORGIO GRIBAUDO ◽  
FRANCA COFANO ◽  
MARIA PRAT ◽  
SANTO LANDOLFO
Keyword(s):  
Monoclonal Antibodies ◽  
Molecular Characterization ◽  
Affinity Purification ◽  
Interferon Γ

scholarly journals Molecular characterization of a virulence-associated epitope on the lipopolysaccharide ofLegionella pneumophilaserogroup 1

1995 ◽  
Vol 115 (1) ◽  
pp. 71-78 ◽  
Author(s):  
J. H. Helbig ◽  
P. C. Lück ◽  
Y. A. Knirel ◽  
W. Witzleb ◽  
U. Zähringer
Keyword(s):  
Monoclonal Antibodies ◽  
Molecular Characterization ◽  
Legionella Pneumophila ◽  
Reactivity Pattern ◽  
Virulence Marker ◽  
Nonulosonic Acid ◽  
Acetyl Group

SummaryFor identification of lipopolysaccharide (LPS)-associated epitopes ofLegionella pneumophilaserogroup 1, LPS of strain Philadelphia 1 was investigated using monoclonal antibodies (MAbs). The O-specific chain of LPS is a homopolymer of 5-acetamidino-7-acetamido-8-O-acetyl-3,5,7,9-tetradeoxy-D-glycero-L-galacto-nonulosonic acid. At least four immunoaccessible epitopes were recognized by different MAbs on the intact LPS. AfterO-deacetylation of LPS, the reactivity of one of the MAbs (MAb 3/1) was lost, indicating thus that the corresponding epitope is associated with the 8-O-acetyl group. Since the reactivity pattern of the MAb 3/1 is identical with those of the MAb 2 which was considered as a virulence marker for serogroup 1, this epitope may be involved in mediating virulence inL. pneumophila. Four MAbs specific to strains of serogroup 1 other than the monoclonal subtype Philadelphia recognized epitopes on theO-deacetylated LPS of strain Philadelphia 1 and, therefore, the virulence-associated epitope blocks recognition of the immunodeterminants that are accessible on the intact LPS of the strains lacking this epitope.

Molecular Characterization of Variable Heavy and Light Chain Regions of Five HIV Type 1-Specific Human Monoclonal Antibodies*

1994 ◽  
Vol 10 (12) ◽  
pp. 1639-1649 ◽  
Author(s):  
ERIC M.M. van der DONK ◽  
MARTIN SCHUTTEN ◽  
ALBERT D.M.E. OSTERHAUS ◽  
ROGER W.J. van der HEIJDEN
Keyword(s):  
Monoclonal Antibodies ◽  
Molecular Characterization ◽  
Light Chain ◽  
Human Monoclonal Antibodies ◽  
Variable Heavy ◽  
Hiv Type 1

Molecular characterization of chicken prion proteins by C-terminal-specific monoclonal antibodies

2009 ◽  
Vol 128 (4) ◽  
pp. 402-406 ◽  
Author(s):  
Naotaka Ishiguro ◽  
Yasuo Inoshima ◽  
Yukiko Sassa ◽  
Toyomi Takahashi
Keyword(s):  
Monoclonal Antibodies ◽  
Molecular Characterization ◽  
Prion Proteins

Characterization of Monoclonal Antibodies Recognizing Human Merosin and Their Use in Affinity Purification of Native Merosin

Hybridoma ◽  
1996 ◽  
Vol 15 (5) ◽  
pp. 373-378 ◽  
Author(s):  
TOSHIHIKO MIZUTA ◽  
HISAKI SHIMADA ◽  
KATSUHIKO ARAI ◽  
HISAE HORI ◽  
SHUNJI HATTORI ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Affinity Purification

Molecular Characterization of South American Bovine Herpesvirus-1 Isolates with Monoclonal Antibodies and SDS-PAGE

1993 ◽  
Vol 40 (1-10) ◽  
pp. 125-130 ◽  
Author(s):  
A. Suarez Heinlein ◽  
A. E. Metzler ◽  
R. Weiblen ◽  
P. Berrios ◽  
A. A. Schudel ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Molecular Characterization ◽  
Bovine Herpesvirus ◽  
South American ◽  
Bovine Herpesvirus 1 ◽  
Sds Page ◽  
Herpesvirus 1
Sign in / Sign up

Export Citation Format

Share Document