Characterization of a Late Expression Gene of Bombyx mori Nucleopolyhedrovirus

Bombyx mori nucleopolyhedrovirus (BmNPV) ORF5 (Bm5) is a gene present in many lepidopteran nucleopolyhedroviruses (NPVs), but its function is unknown. In this study, Bm5 was characterized. The transcript of Bm5 was detected 12 - 72 h post infection (p.i.). Polyclonal antiserum raised to a His-BM5 fusion protein recognized BM5 in infected cell lysates from 24 to 72 h p.i., suggesting that Bm5 is a late gene. Immunofluorescence analysis by confocal microscopy showed that the BM5 protein is localized primarily in the cytoplasm. Localization of BM5 in budded virion (BV) and occlusion-derived virion (ODV) by Western analyses demonstrated that BM5 is not a structural protein associated with BV or ODV.

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Characterization of Bombyx mori Nucleopolyhedrovirus orf68 Gene That Encodes a Novel Structural Protein of Budded Virus

Virology ◽

10.1006/viro.2002.1443 ◽

2002 ◽

Vol 297 (1) ◽

pp. 39-47 ◽

Cited By ~ 19

Author(s):

Masashi Iwanaga ◽

Masaaki Kurihara ◽

Masahiko Kobayashi ◽

WonKyung Kang

Keyword(s):

Bombyx Mori ◽

Structural Protein ◽

Bombyx Mori Nucleopolyhedrovirus ◽

Budded Virus

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Characterization of a Late Gene, ORF60 from Bombyx mori Nucleopolyhedrovirus

BMB Reports ◽

10.5483/bmbrep.2006.39.6.737 ◽

2006 ◽

Vol 39 (6) ◽

pp. 737-742 ◽

Cited By ~ 4

Author(s):

Meng-Fang Du ◽

Xin-Ming Yin ◽

Zhong-Jian Guo ◽

Liang-Jun Zhu

Keyword(s):

Bombyx Mori ◽

Late Gene ◽

Bombyx Mori Nucleopolyhedrovirus

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Characterization of a late gene, ORF75 from Bombyx mori nucleopolyhedrovirus

Molecular Biology Reports ◽

10.1007/s11033-010-0341-6 ◽

2010 ◽

Vol 38 (3) ◽

pp. 2141-2149 ◽

Cited By ~ 2

Author(s):

Jun-Qing Ge ◽

Guo-Hui Gao ◽

Yi-Peng Xu ◽

Chuan-Xi Zhang

Keyword(s):

Bombyx Mori ◽

Late Gene ◽

Bombyx Mori Nucleopolyhedrovirus

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Bombyx mori Nucleopolyhedrovirus p26 Is Associated with Viral Late Stage Replication

Insects ◽

10.3390/insects12080707 ◽

2021 ◽

Vol 12 (8) ◽

pp. 707

Author(s):

Jun-Qing Ge ◽

Zhu-Hong Wang ◽

Xi Chen ◽

Hua Chen ◽

Jian Huang

Keyword(s):

Infected Cell ◽

Bombyx Mori ◽

Infectious Virus ◽

Pcr Analysis ◽

Immunofluorescence Analysis ◽

Bombyx Mori Nucleopolyhedrovirus ◽

Cell Cytoplasm ◽

Infected Cells ◽

Budded Virus ◽

Infection Stage

Bombyx mori nucleopolyhedrovirus (BmNPV) p26 is conserved among all Lepidoptera baculoviruses that have been completely sequenced thus far, and some baculoviruses even have two copies of p26, which suggested that p26 may play an important role in the virus infection cycle. This study aimed to characterize BmNPV p26. We found that BmNPV p26 transcripts were detectable as early as 3 h post-infection (hpi), and the transcript levels rapidly increased starting from 12 hpi. Western blot analysis using an anti-p26 polyclonal antibody demonstrated that the corresponding protein was also detectable from 6 hpi in BmNPV-infected cell lysates. Immunofluorescence analysis demonstrated that p26 was mainly dispersed in the infected cell cytoplasm, whereas the over-expressed fusion protein EGFP-p26 also accumulated in the nucleus. These results indicated that p26 is an early BmNPV gene and has functions both in the cytoplasm and the nucleus. RNAi-based knockdown of p26 could produce infectious virus and normal-appearing virions but decreased budded virus (BV) production in BmNPV-infected cells at 72 hpi. Moreover, the results of further quantitative PCR (Q-PCR) analysis indicated that the gp64 and p74 transcripts levels decreased significantly. These results indicated that BmNPV p26 may be associated with BmNPV replication during the late infection stage.

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