Test Method for Carbon Tetrachloride and Chloroform in Liquid Chlorine by Direct Injection (Gas Chromatographic Procedure)

Abstract Presently, disulfram is used in aversion therapy for recovering alcoholics. It acts by inhibiting aldehyde dehydrogenase, leading to high blood levels of acetaldehyde. A simple direct injection micellar liquid chromatographic procedure was developed to determine disulfram in illicit preparations (ayurvedic, herbal, divine ash, and traditional medicine), as well as inpharmaceuticals and biological samples (urine). After application of a predictive optimization strategy, the proposed method was developed using a 0.1 M sodium dodecyl sulfate-butanol 4% (v/v) buffered to pH 7 as the mobile phase at a ﬂow rate of 1 mL/min, an octyl silyl (C8) 150 mm column, and diode array detection at 248 nm. Under the above conditions, the analysis time was below 8 min. Validation studies were based on U.S. Food and Drug Administration guidelines. The LOD (3 × SD criterion) was 15 ng/mL and LOQ (10 × SD criterion) was 70 ng/mL for disulfram. The intraday and interday precisions were below 3.5%, recoveries were in the range of 97–102%, and robustness was below 3%. The optimized and validated micellar liquid chromatographic method was successfully applied to the determination of disulfram in ayurvedic, herbal, divine ash, and other samples. The procedure developed could also be used in the felds of QC, routine analysis, and pharmacokinetic studies.

Substitutes for Carbon Tetrachloride in the Standard ASTM Test Method for Activity of Activated Carbon

Journal of the Air Pollution Control Association ◽

10.1080/00022470.1984.10465873 ◽

1984 ◽

Vol 34 (12) ◽

pp. 1215-1217

Author(s):

Atilio Sosa ◽

Dwight Underbill

Keyword(s):

Activated Carbon ◽

Carbon Tetrachloride ◽

Test Method

Test Method for Water Content of Coatings by Direct Injection Into a Gas Chromatograph

10.1520/d3792-05r09 ◽

2009 ◽

Author(s):

Keyword(s):

Water Content ◽

Direct Injection ◽

Test Method ◽

Gas Chromatograph