Analysis of the mutant picornavirus RNA-dependent RNA polymerase with disrupted primary and secondary RNA structure (RdRPΔrna)-dependent transcriptome in human THP-1 monocytic leukemia cells

2017 ◽  
Author(s):  
EM Poeschla ◽  
M Rodriguez
Keyword(s):  
Rna Polymerase ◽  
Rna Structure ◽  
Leukemia Cells ◽  
Monocytic Leukemia ◽  
Rna Dependent Rna Polymerase

scholarly journals Analysis of the picornavirus RNA-dependent RNA polymerase (RdRP)-dependent transcriptome in human THP-1 monocytic leukemia cells

2017 ◽  
Author(s):  
MM Painter ◽  
JH Morrison ◽  
LJ Zoecklein ◽  
TA Rinkoski ◽  
JO Watzlawik ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Leukemia Cells ◽  
Monocytic Leukemia ◽  
Rna Dependent Rna Polymerase

scholarly journals Isolation of an RNA-dependent RNA Polymerase from Friend Murine Leukemia Cells

1970 ◽  
Vol 46 (10) ◽  
pp. 1016-1021 ◽  
Author(s):  
Ichiro HARUNA ◽  
Tsuneya OHNO ◽  
Itaru WATANABE ◽  
Yoji IKAWA
Keyword(s):  
Rna Polymerase ◽  
Leukemia Cells ◽  
Rna Dependent Rna Polymerase ◽  
Murine Leukemia

scholarly journals A cis-Acting Replication Element in the Sequence Encoding the NS5B RNA-Dependent RNA Polymerase Is Required for Hepatitis C Virus RNA Replication

2004 ◽  
Vol 78 (3) ◽  
pp. 1352-1366 ◽  
Author(s):  
Shihyun You ◽  
Decherd D. Stump ◽  
Andrea D. Branch ◽  
Charles M. Rice
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Rna Polymerase ◽  
Rna Structure ◽  
Mutational Analysis ◽  
Rna Replication ◽  
Site Directed Mutagenesis ◽  
Hcv Rna ◽  
Rna Dependent Rna Polymerase ◽  
Structure Probing

ABSTRACT RNA structures play key roles in the replication of RNA viruses. Sequence alignment software, thermodynamic RNA folding programs, and classical comparative phylogenetic analysis were used to build models of six RNA elements in the coding region of the hepatitis C virus (HCV) RNA-dependent RNA polymerase, NS5B. The importance of five of these elements was evaluated by site-directed mutagenesis of a subgenomic HCV replicon. Mutations disrupting one of the predicted stem-loop structures, designated 5BSL3.2, blocked RNA replication, implicating it as an essential cis-acting replication element (CRE). 5BSL3.2 is about 50 bases in length and is part of a larger predicted cruciform structure (5BSL3). As confirmed by RNA structure probing, 5BSL3.2 consists of an 8-bp lower helix, a 6-bp upper helix, a 12-base terminal loop, and an 8-base internal loop. Mutational analysis and structure probing were used to explore the importance of these features. Primary sequences in the loops were shown to be important for HCV RNA replication, and the upper helix appears to serve as an essential scaffold that helps maintain the overall RNA structure. Unlike certain picornavirus CREs, whose function is position independent, 5BSL3.2 function appears to be context dependent. Understanding the role of 5BSL3.2 and determining how this new CRE functions in the context of previously identified elements at the 5′ and 3′ ends of the RNA genome should provide new insights into HCV RNA replication.

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