scholarly journals Chloroform-Methanol Protein Extraction with Bead Beating for Yeast v1 (protocols.io.btj3nkqn)

protocols.io ◽  
2021 ◽  
Author(s):  
Leanne Chan ◽  
Christopher Petzold
Keyword(s):  
Protein Extraction ◽  
Bead Beating ◽  
Chloroform Methanol

Automated Chloroform-Methanol Protein Extraction on the Biomek-FX Liquid Handler System v2

2022 ◽  
Author(s):  
Yan Chen ◽  
Tad Ogorzalek ◽  
Nurgul Kaplan Lease ◽  
Jennifer Gin ◽  
Christopher J Petzold
Keyword(s):  
Sample Preparation ◽  
Protein Extraction ◽  
Tryptic Digestion ◽  
Gram Negative ◽  
Protein Quantitation ◽  
System P ◽  
Automated Protocol ◽  
Chloroform Methanol ◽  
Extract Protein

This protocol details steps to extract protein from Gram-negative bacterial or fungal cells (that have been pretreated with zymolyase) in quantitative proteomic workflows by using a Biomek FX liquid handler system. It is a semi-automated protocol that includes several 'pause' steps for centrifugation steps that are conducted manually "off-deck". This protocol works best as part of an automated proteomic sample preparation workflow with: Automated Protein Quantitation with the Biomek-FX liquid handler system and Automated Protein Normalization and Tryptic Digestion on a Biomek-NX Liquid Handler System

Automated Chloroform-Methanol Protein Extraction on the Biomek-FX Liquid Handler System v1

2020 ◽  
Author(s):  
Yan Chen ◽  
Tad Ogorzalek ◽  
Nurgul Kaplan Lease ◽  
Jennifer Gin ◽  
Christopher J Petzold
Keyword(s):  
Sample Preparation ◽  
Protein Extraction ◽  
Tryptic Digestion ◽  
Gram Negative ◽  
Protein Quantitation ◽  
System P ◽  
Automated Protocol ◽  
Chloroform Methanol ◽  
Extract Protein

This protocol details steps to extract protein from Gram-negative bacterial or fungal cells (that have been pretreated with zymolyase) in quantitative proteomic workflows by using a Biomek FX liquid handler system. It is a semi-automated protocol that includes several 'pause' steps for centrifugation steps that are conducted manually "off-deck". This protocol works best as part of an automated proteomic sample preparation workflow with: Automated Protein Quantitation with the Biomek-FX liquid handler system and Automated Protein Normalization and Tryptic Digestion on a Biomek-NX Liquid Handler System

Automated Protein Normalization and Tryptic Digestion on a Biomek-NX Liquid Handler System v2

2022 ◽  
Author(s):  
Yan Chen ◽  
Tad Ogorzalek ◽  
Nurgul Kaplan Lease ◽  
Jennifer Gin ◽  
Christopher J Petzold
Keyword(s):  
Sample Preparation ◽  
Protein Extraction ◽  
Tryptic Digestion ◽  
Protein Quantitation ◽  
System P ◽  
Chloroform Methanol

This protocol details steps to normalize the amount of protein for tryptic digestion in quantitative proteomic workflows by using a Biomek NX liquid handler system. It is optimized to normalize protein concentrations in a 96-well plate format and add TCEP, IAA, and trypsin. This protocol works best as part of a semi-automated proteomic sample preparation workflow with: Automated Chloroform-Methanol Protein Extraction on the Biomek-FX Liquid Handler System and Automated Protein Quantitation with the Biomek-FX liquid handler system

Automated Protein Quantification with the Biomek-FX Liquid Handler System v1

2021 ◽  
Author(s):  
Yan Chen ◽  
Nurgul Kaplan Lease ◽  
Tad Ogorzalek ◽  
Jennifer Gin ◽  
Christopher J Petzold
Keyword(s):  
Sample Preparation ◽  
Protein Extraction ◽  
Protein Quantification ◽  
Tryptic Digestion ◽  
System P ◽  
Plate Reader ◽  
Separate Control ◽  
Control Plate ◽  
Chloroform Methanol

This protocol details steps to perform the protein quantification (Lowry-based) assay by using a Biomek FX liquid handler system. It is optimized to assay a full 96-well plate of protein samples in duplicate with a separate (control) plate for BSA standards. You will need a plate reader to measure the samples and standards. This protocol works best as part of a full proteomic sample preparation workflow with: Automated Chloroform-Methanol Protein Extraction on the Biomek-FX Liquid Handler System and Automated Protein Normalization and Tryptic Digestion on a Biomek-FX Liquid Handler System

Automated Protein Normalization and Tryptic Digestion on a Biomek-NX Liquid Handler System v1

2021 ◽  
Author(s):  
Yan Chen ◽  
Tad Ogorzalek ◽  
Nurgul Kaplan Lease ◽  
Jennifer Gin ◽  
Christopher J Petzold
Keyword(s):  
Sample Preparation ◽  
Protein Extraction ◽  
Tryptic Digestion ◽  
Protein Quantitation ◽  
System P ◽  
Chloroform Methanol

This protocol details steps to normalize the amount of protein for tryptic digestion in quantitative proteomic workflows by using a Biomek NX liquid handler system. It is optimized to normalize protein concentrations in a 96-well plate format and add TCEP, IAA, and trypsin. This protocol works best as part of a semi-automated proteomic sample preparation workflow with: Automated Chloroform-Methanol Protein Extraction on the Biomek-FX Liquid Handler System and Automated Protein Quantitation with the Biomek-FX liquid handler system

Automated Protein Quantification with the Biomek-FX Liquid Handler System v2

2022 ◽  
Author(s):  
Yan Chen ◽  
Nurgul Kaplan Lease ◽  
Tad Ogorzalek ◽  
Jennifer Gin ◽  
Christopher J Petzold
Keyword(s):  
Sample Preparation ◽  
Protein Extraction ◽  
Protein Quantification ◽  
Tryptic Digestion ◽  
System P ◽  
Plate Reader ◽  
Separate Control ◽  
Control Plate ◽  
Chloroform Methanol

This protocol details steps to perform the protein quantification (Lowry-based) assay by using a Biomek FX liquid handler system. It is optimized to assay a full 96-well plate of protein samples in duplicate with a separate (control) plate for BSA standards. You will need a plate reader to measure the samples and standards. This protocol works best as part of a full proteomic sample preparation workflow with: Automated Chloroform-Methanol Protein Extraction on the Biomek-FX Liquid Handler System and Automated Protein Normalization and Tryptic Digestion on a Biomek-FX Liquid Handler System

Sign in / Sign up

Export Citation Format

Share Document