Rapid PCR Method for Site-Directed Mutagenesis on Double-Stranded Plasmid DNA

BioTechniques ◽

10.2144/96201bm09 ◽

1996 ◽

Vol 20 (1) ◽

pp. 44-46 ◽

Author(s):

X. Xu ◽

S.-H. Kang ◽

O. Heidenreich ◽

Q. Li ◽

M. Nerenberg

Keyword(s):

Plasmid Dna ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Rapid Pcr ◽

Download Full-text

Site-Directed Mutagenesis Using Double-Stranded Plasmid DNA Templates

In Vitro Mutagenesis Protocols ◽

10.1385/0-89603-332-5:31 ◽

2003 ◽

pp. 31-44 ◽

Author(s):

Jeffrey Braman ◽

Carol Papworth ◽

Alan Greener

Keyword(s):

Plasmid Dna ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Download Full-text

Totalin VitroSynthesis of Plasmid DNA Facilitates Site-Directed Mutagenesis

Analytical Biochemistry ◽

10.1006/abio.1995.0017 ◽

1995 ◽

Vol 232 (2) ◽

pp. 254-258 ◽

Author(s):

Rolf Postina ◽

Falk Fahrenholz

Keyword(s):

Plasmid Dna ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis

Download Full-text

A Simple Method for Site-Directed Mutagenesis with Double-Stranded Plasmid DNA

In Vitro Mutagenesis Protocols ◽

10.1385/0-89603-332-5:75 ◽

2003 ◽

pp. 75-86

Author(s):

Derhsing Lai ◽

Sidney Pestka

Keyword(s):

Plasmid Dna ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Download Full-text

Rapid and efficient site-directed mutagenesis by the single-tube megaprimer PCR method

Nature Methods ◽

10.1038/nmeth1104-181 ◽

2004 ◽

Vol 1 (2) ◽

pp. 181-182

Keyword(s):

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Single Tube ◽

Download Full-text

Asymmetric overlap extension PCR method bypassing intermediate purification and the amplification of wild-type template in site-directed mutagenesis

Biotechnology Letters ◽

10.1007/s10529-007-9327-4 ◽

2007 ◽

Vol 29 (6) ◽

pp. 925-930 ◽

Author(s):

Yue-Hua Xiao ◽

Meng-Hui Yin ◽

Lei Hou ◽

Ming Luo ◽

Yan Pei

Keyword(s):

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Wild Type ◽

Overlap Extension Pcr ◽

Overlap Extension ◽

Download Full-text

Rapid and efficient site-directed mutagenesis by single-tube 'megaprimer' PCR method

Nucleic Acids Research ◽

10.1093/nar/25.16.3371 ◽

1997 ◽

Vol 25 (16) ◽

pp. 3371-3372 ◽

Author(s):

S. Ke

Keyword(s):

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Single Tube ◽

Download Full-text

Fast and Almost 100% Efficiency Site-directed Mutagenesis by The Megaprimer PCR Method*

PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS ◽

10.3724/sp.j.1206.2009.00139 ◽

2009 ◽

Vol 36 (11) ◽

pp. 1490-1494 ◽

Author(s):

Zhen-Hua XIE ◽

Xiao-Jun SHI

Keyword(s):

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Download Full-text

A simple and efficient method for site-directed mutagenesis with double-stranded plasmid DNA

Nucleic Acids Research ◽

10.1093/nar/21.17.3977 ◽

1993 ◽

Vol 21 (17) ◽

pp. 3977-3980 ◽

Author(s):

Derhsing Lai ◽

Xueli Zhu ◽

Sidney Pestka

Keyword(s):

Efficient Method ◽

Plasmid Dna ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis

Download Full-text

Site-Directed Mutagenesis Using a Rapid PCR-Based Method

In Vitro Mutagenesis Protocols ◽

10.1385/0-89603-332-5:239 ◽

2003 ◽

pp. 239-248 ◽

Author(s):

Gina L. Costa ◽

John C. Bauer ◽

Barbara McGowan ◽

Mila Angert ◽

Michael P. Weiner

Keyword(s):

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Download Full-text

Rapid and Efficient Site-directed Mutagenesis by the Single-tube Megaprimer PCR Method

Cold Spring Harbor Protocols ◽

10.1101/pdb.prot3467 ◽

2006 ◽

Vol 2006 (1) ◽

pp. pdb.prot3467 ◽

Author(s):

Joseph Sambrook ◽

David W. Russell

Keyword(s):

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Single Tube ◽

Download Full-text