scholarly journals EFFECTIVENESS OF LOW SPEED VENTILATION CONTROL IN TUNNEL FIRES (INFLUENCE OF LONGITUDINAL INCLINATION TO THERMAL FUME BEHAVIOUR)

Author(s):  
Shinji EMOTO ◽  
Miho SEIKE ◽  
Nobuyoshi KAWABATA ◽  
Masato HASEGAWA
2016 ◽  
Vol 2016 (0) ◽  
pp. S0540401
Author(s):  
Shinji EMOTO ◽  
Miho SEIKE ◽  
Nobuyoshi KAWABATA ◽  
Masato HASEGAWA

2017 ◽  
Vol 87 ◽  
pp. 25-36 ◽  
Author(s):  
W. Ulises Rojas Alva ◽  
Grunde Jomaas ◽  
Anne S. Dederichs

Author(s):  
S. F. Hayes ◽  
M. D. Corwin ◽  
T. G. Schwan ◽  
D. W. Dorward ◽  
W. Burgdorfer

Characterization of Borrelia burgdorferi strains by means of negative staining EM has become an integral part of many studies related to the biology of the Lyme disease organism. However, relying solely upon negative staining to compare new isolates with prototype B31 or other borreliae is often unsatisfactory. To obtain more satisfactory results, we have relied upon a correlative approach encompassing a variety EM techniques, i.e., scanning for topographical features and cryotomy, negative staining and thin sectioning to provide a more complete structural characterization of B. burgdorferi.For characterization, isolates of B. burgdorferi were cultured in BSK II media from which they were removed by low speed centrifugation. The sedimented borrelia were carefully resuspended in stabilizing buffer so as to preserve their features for scanning and negative staining. Alternatively, others were prepared for conventional thin sectioning and for cryotomy using modified procedures. For thin sectioning, the fixative described by Ito, et al.


1994 ◽  
Vol 12 (4) ◽  
pp. 623-629 ◽  
Author(s):  
Andreas Hartmann ◽  
Martin Kaltenbach
Keyword(s):  

2014 ◽  
Vol 134 (1) ◽  
pp. 106-114
Author(s):  
Sari Maekawa ◽  
Toshifumi Hinata ◽  
Nobuyuki Suzuki ◽  
Hisao Kubota

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