scholarly journals Genetic Characterization of Rhizoctonia solani AG-2-3 by Analyzing Restriction Fragment Length Polymorphisms of Nuclear Ribosomal DNA Internal Transcribed Spacers.

1995 ◽  
Vol 61 (1) ◽  
pp. 18-21 ◽  
Author(s):  
Seiji KANEMATSU ◽  
Shigeo NAITO
Keyword(s):  
Rhizoctonia Solani ◽  
Restriction Fragment ◽  
Ribosomal Dna ◽  
Fragment Length ◽  
Genetic Characterization ◽  
Internal Transcribed Spacers ◽  
Nuclear Ribosomal Dna ◽  
Length Polymorphisms ◽  
Restriction Fragment Length

Studies on keratinophilic fungi. X. Arthrographis alba sp.nov.

1996 ◽  
Vol 42 (12) ◽  
pp. 1185-1189 ◽  
Author(s):  
Josepa Gené ◽  
Josep Guarro ◽  
José Manuel Guillamón ◽  
Krzysztof Ulfig
Keyword(s):  
Restriction Fragment ◽  
Ribosomal Dna ◽  
Fragment Length ◽  
Restriction Fragment Length Polymorphisms ◽  
Keratinophilic Fungi ◽  
Length Polymorphisms ◽  
Restriction Fragment Length

In our studies on keratinophilic fungi in Spain, an arthroconidial anamorphic species has been recovered and is herein described as new. Arthrographis alba sp.nov. is characterized by having arthroconidia borne from laterally or pseudodichotomously branched conidiophores, white colonies, and nil or restricted growth at 37 °C. Analysis of restriction fragment length polymorphisms of ribosomal DNA reveals differences with Arthrographis kalrae, a morphologically similar species.Key words: Arthrographis alba, Hyphomycetes, rDNA.

Restriction fragment length polymorphisms in polymerase chain reaction amplified ribosomal DNAs of three Trichogramma (Hymenoptera: Trichogrammatidae) species

Genome ◽  
1995 ◽  
Vol 38 (3) ◽  
pp. 419-425 ◽  
Author(s):  
Navtej Pal Sappal ◽  
Robert S. Jeng ◽  
Martin Hubbes ◽  
Fuhua Liu
Keyword(s):  
Restriction Fragment ◽  
Ribosomal Dna ◽  
Restriction Enzyme ◽  
Fragment Length ◽  
Its Region ◽  
Restriction Fragment Length Polymorphisms ◽  
Length Variation ◽  
Length Polymorphisms ◽  
Ribosomal Dnas ◽  
Restriction Fragment Length

Restriction fragment length polymorphisms from PCR amplified ribosomal DNAs of three Trichogramma species, T. minutum, T. brassicae, and T. near sibiricum, were studied. Length variation in the internal transcribed spacer (ITS) region was observed. The ITS region of T. brassicae is about 1350 base pairs (bp) in length and those of T. minutum and T. near sibiricum are 1300 bp. These three species also differ in the size of their ITS1 and ITS2 regions. Restriction enzyme digestions of these regions showed unique banding patterns for each species. The amplified 18S region of ribosomal DNA is about 1800 bp in length and showed no length variation between the three species of Trichogramma. Restriction enzyme digestion of this region by BamHI differentiated T. brassicae from the other two species. Restriction site maps of the ITS and 18S regions were constructed for each species. The amplified 28S region is about 1700 bp for these three species. Restriction of this region by RsaI and SacII differentiates these three species. The reported results indicate that these species of Trichogramma can be clearly differentiated from one another by nuclear ribosomal DNA markers.Key words: rDNA, Trichogramma, PCR.

Isolation and characterization of DNA probes from the short arm of the human X chromosome that detect restriction fragment length polymorphisms

Genome ◽  
1987 ◽  
Vol 29 (1) ◽  
pp. 201-205 ◽  
Author(s):  
Terence Starr ◽  
Stephen Wood
Keyword(s):  
X Chromosome ◽  
Restriction Fragment ◽  
Fragment Length ◽  
Restriction Fragment Length Polymorphisms ◽  
Somatic Cell Hybrids ◽  
Key Words Dna ◽  
Isolation And Characterization ◽  
Length Polymorphisms ◽  
Restriction Fragment Length

We have isolated 30 X chromosome specific probes from a flow-sorted library enriched for the human X chromosome. Hybridization to somatic cell hybrids containing different regions of the X chromosome localized nine of these probes to Xp. After testing 185 probe–enzyme combinations, three of the Xp probes were found to detect restriction fragment length polymorphisms. Key words: DNA probe, polymorphisms (restriction), X chromosome.

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