scholarly journals Phylogenetic relationships among Acanthamoeba spp. based on PCR-RFLP analyses of mitochondrial small subunit rRNA gene

1999 ◽  
Vol 37 (3) ◽  
pp. 181 ◽  
Author(s):  
Hak-sun Yu ◽  
Mee-yul Hwang ◽  
Tae-ook Kim ◽  
Ho-cheol Yun ◽  
Tae-ho Kim ◽  
...  
Keyword(s):  
Phylogenetic Relationships ◽  
Small Subunit ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Small Subunit Rrna Gene ◽  
Pcr Rflp

Phylogenetic Relationships within the Class Spirotrichea (Ciliophora) Inferred from Small Subunit rRNA Gene Sequences

2001 ◽  
Vol 21 (1) ◽  
pp. 86-92 ◽  
Author(s):  
Detlef Bernhard ◽  
Alexandra Stechmann ◽  
Wilhelm Foissner ◽  
Dieter Ammermann ◽  
Manuela Hehn ◽  
...  
Keyword(s):  
Phylogenetic Relationships ◽  
Small Subunit ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Gene Sequences ◽  
Small Subunit Rrna Gene

scholarly journals Genetic Characterization of Cryptosporidium cuniculus from Rabbits in Egypt

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 775
Author(s):  
Doaa Naguib ◽  
Dawn M. Roellig ◽  
Nagah Arafat ◽  
Lihua Xiao
Keyword(s):  
Rflp Analysis ◽  
Small Subunit ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Genetic Characteristics ◽  
Glycoprotein Gene ◽  
Cryptosporidium Spp ◽  
Small Subunit Rrna Gene ◽  
Pcr Rflp

Rabbits are increasingly farmed in Egypt for meat. They are, however, known reservoirs of infectious pathogens. Currently, no information is available on the genetic characteristics of Cryptosporidium spp. in rabbits in Egypt. To understand the prevalence and genetic identity of Cryptosporidium spp. in these animals, 235 fecal samples were collected from rabbits of different ages on nine farms in El-Dakahlia, El-Gharbia, and Damietta Provinces, Egypt during the period from July 2015 to April 2016. PCR-RFLP analysis of the small subunit rRNA gene was used to detect and genotype Cryptosporidium spp. The overall detection rate was 11.9% (28/235). All 28 samples were identified as Cryptosporidium cuniculus. The 16 samples successfully subtyped by the sequence analysis of the partial 60 kDa glycoprotein gene belonged to two subtypes, VbA19 (n = 1) and VbA33 (n = 15). As C. cuniculus is increasingly recognized as a cause of human cryptosporidiosis, Cryptosporidium spp. in rabbits from Egypt have zoonotic potential.

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