scholarly journals Genetic Characterization of Cryptosporidium cuniculus from Rabbits in Egypt

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 775
Author(s):  
Doaa Naguib ◽  
Dawn M. Roellig ◽  
Nagah Arafat ◽  
Lihua Xiao
Keyword(s):  
Rflp Analysis ◽  
Small Subunit ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Genetic Characteristics ◽  
Glycoprotein Gene ◽  
Cryptosporidium Spp ◽  
Small Subunit Rrna Gene ◽  
Pcr Rflp

Rabbits are increasingly farmed in Egypt for meat. They are, however, known reservoirs of infectious pathogens. Currently, no information is available on the genetic characteristics of Cryptosporidium spp. in rabbits in Egypt. To understand the prevalence and genetic identity of Cryptosporidium spp. in these animals, 235 fecal samples were collected from rabbits of different ages on nine farms in El-Dakahlia, El-Gharbia, and Damietta Provinces, Egypt during the period from July 2015 to April 2016. PCR-RFLP analysis of the small subunit rRNA gene was used to detect and genotype Cryptosporidium spp. The overall detection rate was 11.9% (28/235). All 28 samples were identified as Cryptosporidium cuniculus. The 16 samples successfully subtyped by the sequence analysis of the partial 60 kDa glycoprotein gene belonged to two subtypes, VbA19 (n = 1) and VbA33 (n = 15). As C. cuniculus is increasingly recognized as a cause of human cryptosporidiosis, Cryptosporidium spp. in rabbits from Egypt have zoonotic potential.

scholarly journals Characterization of two urostylid ciliates, Metaurostylopsis flavicana spec. nov. and Tunicothrix wilberti (Lin & Song, 2004) Xu et al., 2006 (Ciliophora, Stichotrichia), from a mangrove nature protection area in China

2011 ◽  
Vol 61 (7) ◽  
pp. 1740-1750 ◽  
Author(s):  
Yangang Wang ◽  
Xiaozhong Hu ◽  
Jie Huang ◽  
Khaled A. S. Al-Rasheid ◽  
Alan Warren
Keyword(s):  
Phylogenetic Trees ◽  
Small Subunit ◽  
The Body ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Cortical Granules ◽  
Nature Protection ◽  
Small Subunit Rrna Gene ◽  
Protection Area

Two marine stichotrich ciliates, Metaurostylopsis flavicana spec. nov. and Tunicothrix wilberti (Lin & Song, 2004) Xu et al., 2006, isolated from the Shenzhen Mangrove Protection Area on the coast of the South China Sea, were investigated using live observation and protargol impregnation techniques. Metaurostylopsis flavicana is characterized by its elongate body shape, yellowish body colour and bright-yellow cortical granules that are either grouped around the cirri and the dorsal cilia or aligned between the rows of cirri and dorsal cilia. It has four to eight frontal, two frontoterminal, one buccal and seven to ten transverse cirri, a mid-ventral complex comprising 13–17 midventral cirral pairs in a row that extends about three-fifths of the body length, four left and three right marginal rows and three complete dorsal kineties. The small subunit rRNA gene of this species was sequenced and phylogenetic trees were constructed in which M. flavicana does not group with its congeners, suggesting that the genus Metaurostylopsis is paraphyletic. Some supplementary morphological and morphogenetic traits for Tunicothrix wilberti are also documented.

Small-subunit rRNA gene sequences from representatives of selected families of the Gigartinales and Rhodymeniales (Rhodophyta). 2. Recognition of the Halymeniales ord.nov.

1996 ◽  
Vol 74 (5) ◽  
pp. 694-707 ◽  
Author(s):  
G. W. Saunders ◽  
G. T. Kraft
Keyword(s):  
Phylogenetic Analyses ◽  
Molecular Data ◽  
Small Subunit ◽  
Botanical Nomenclature ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Small Subunit Rrna Gene ◽  
Suggested Keywords ◽  
Polymerase Chain

Nucleotide sequences of the nuclear, small-subunit ribosomal RNAs, as inferred from polymerase chain reaction amplified products, are introduced for representatives of the Dumontiaceae, Endocladiaceae, Halymeniaceae, and Kallymeniaceae of the order Cryptonemiales sensu Kylin, the Mychodeaceae, Phyllophoraceae, Schizymeniaceae, and Sebdeniaceae of the order Gigartinales sensu Kylin, and the Lomentariaceae and Rhodymeniaceae of the order Rhodymeniales. The new sequences are included in phylogenetic analyses incorporating previously published sequences from additional families of the orders Ahnfeltiales, Ceramiales, Gigartinales, Gracilariales, Palmariales, Plocamiales, and Rhodymeniales. We used the molecular data to test the validity of the taxonomic merger of the orders Gigartinales and Cryptonemiales that was proposed by G.T. Kraft and P.A. Robins in 1985. With only two exceptions (the families Halymeniaceae and Sebdeniaceae), phylogenetic analyses of the SSU data support a monophyletic origin for a combined Gigartinales–Cryptonemiales. We therefore propose the resurrection of a redefined Cryptonemiales to consist, at this time, of only the Halymeniaceae and Sebdeniaceae. Because virtually no elements of the original or recent definitions of the Cryptonemiales survive in the characterization of this taxon, we followed procedures allowed by the International Code of Botanical Nomenclature to designate it the Halymeniales ord.nov. Analysis of molecular data further indicates that the Rhodymeniales is a monophyletic assemblage distinct from both the Gigartinales and Halymeniales; it should not be merged with the Gigartinales as is occasionally suggested. Keywords: Cryptonemiales, Gigartinales, Halymeniaceae, Halymeniales, phylogeny, Rhodophyta, Sebdeniaceae, small-subunit rRNA, systematics.

scholarly journals Genetic Diversity of Cryptosporidium spp. in Captive Reptiles

2004 ◽  
Vol 70 (2) ◽  
pp. 891-899 ◽  
Author(s):  
Lihua Xiao ◽  
Una M. Ryan ◽  
Thaddeus K. Graczyk ◽  
Josef Limor ◽  
Lixia Li ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Cryptosporidium Parvum ◽  
Fragment Length Polymorphism ◽  
Small Subunit ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Cryptosporidium Spp ◽  
Small Subunit Rrna Gene ◽  
Different Types ◽  
Restriction Fragment Length

ABSTRACT The genetic diversity of Cryptosporidium in reptiles was analyzed by PCR-restriction fragment length polymorphism and sequence analysis of the small subunit rRNA gene. A total of 123 samples were analyzed, of which 48 snake samples, 24 lizard samples, and 3 tortoise samples were positive for Cryptosporidium. Nine different types of Cryptosporidium were found, including Cryptosporidium serpentis, Cryptosporidium desert monitor genotype, Cryptosporidium muris, Cryptosporidium parvum bovine and mouse genotypes, one C. serpentis-like parasite in a lizard, two new Cryptosporidium spp. in snakes, and one new Cryptosporidium sp. in tortoises. C. serpentis and the desert monitor genotype were the most common parasites and were found in both snakes and lizards, whereas the C. muris and C. parvum parasites detected were probably the result of ingestion of infected rodents. Sequence and biologic characterizations indicated that the desert monitor genotype was Cryptosporidium saurophilum. Two host-adapted C. serpentis genotypes were found in snakes and lizards.

scholarly journals Phylogenetic relationships among Acanthamoeba spp. based on PCR-RFLP analyses of mitochondrial small subunit rRNA gene

1999 ◽  
Vol 37 (3) ◽  
pp. 181 ◽  
Author(s):  
Hak-sun Yu ◽  
Mee-yul Hwang ◽  
Tae-ook Kim ◽  
Ho-cheol Yun ◽  
Tae-ho Kim ◽  
...  
Keyword(s):  
Phylogenetic Relationships ◽  
Small Subunit ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Small Subunit Rrna Gene ◽  
Pcr Rflp

Characterization of Fusarium spp. isolates by PCR-RFLP analysis of the intergenic spacer region of the rRNA gene (rDNA)

2006 ◽  
Vol 106 (3) ◽  
pp. 297-306 ◽  
Author(s):  
A. Llorens ◽  
M.J. Hinojo ◽  
R. Mateo ◽  
M.T. González-Jaén ◽  
F.M. Valle-Algarra ◽  
...  
Keyword(s):  
Intergenic Spacer ◽  
Rflp Analysis ◽  
Rrna Gene ◽  
Fusarium Spp ◽  
Intergenic Spacer Region ◽  
Pcr Rflp

Phylogenetic analysis of Blastocystis isolates from different hosts based on the comparison of small-subunit rRNA gene sequences

2003 ◽  
Vol 126 (1) ◽  
pp. 119-123 ◽  
Author(s):  
Christophe Noël ◽  
Corinne Peyronnet ◽  
Delphine Gerbod ◽  
Virginia P Edgcomb ◽  
Pilar Delgado-Viscogliosi ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Small Subunit ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Gene Sequences ◽  
Small Subunit Rrna Gene

Genetic interrelationships of proteolyticClostridium botulinum types A, B, and F and other members of theClostridium botulinum complex as revealed by small-subunit rRNA gene sequences

1994 ◽  
Vol 64 (3-4) ◽  
pp. 273-283 ◽  
Author(s):  
R. A. Hutson ◽  
D. E. Thompson ◽  
P. A. Lawson ◽  
R. P. Schocken-Itturino ◽  
E. C. B�ttger ◽  
...  
Keyword(s):  
Small Subunit ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Gene Sequences ◽  
Small Subunit Rrna Gene

Free-living nematodes associated with pine cones of Pinus thunbergii and P. taeda at Japanese coastal and inland forest sites

Nematology ◽  
2019 ◽  
Vol 21 (4) ◽  
pp. 389-400 ◽  
Author(s):  
Yudai Kitagami ◽  
Natsumi Kanzaki ◽  
Toko Tanikawa ◽  
Yosuke Matsuda
Keyword(s):  
Gene Sequence ◽  
Small Subunit ◽  
Pinus Thunbergii ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Free Living ◽  
Small Subunit Rrna Gene ◽  
Forest Sites ◽  
High Bootstrap ◽  
Pine Cones

Summary We surveyed the distribution of nematodes in 56 cones of Pinus thunbergii collected from both live branches and on the forest floor in three coastal and inland habitats and in 11 cones of P. taeda collected at different heights. We identified 47 nematodes to family or genera by analysis of an 18S small subunit rRNA gene sequence. The frequencies of occurrence of free-living cone nematodes were 97% in coastal P. thunbergii, 92% in inland P. thunbergii, and 82% in P. taeda. Phylogenetic analysis assigned the nematodes to four clades with high bootstrap values. Nine sequences that were found only in cones on live branches were clustered with Panagrobelus stammeri and an unknown Panagrobelus sp. Our results imply that nematodes are commonly associated with cones in pine forest ecosystems and that a capacity for anhydrobiosis may be a key to surviving above-ground.

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