scholarly journals Screening for Resistance to PVY in Australian Potato Germplasm

Genes ◽  
2020 ◽  
Vol 11 (4) ◽  
pp. 429 ◽  
Author(s):  
Anthony T. Slater ◽  
Lee Schultz ◽  
Maria Lombardi ◽  
Brendan C. Rodoni ◽  
Chris Bottcher ◽  
...  
Keyword(s):  
Potato Breeding ◽  
Potato Virus Y ◽  
Disease Susceptibility ◽  
Limiting Factors ◽  
Food Crop ◽  
Potato Breeding Program ◽  
Extreme Resistance ◽  
Sources Of Resistance ◽  
Potato Germplasm ◽  
Pvy Resistance

Potatoes are an important human food crop, but have a number of yield limiting factors, including disease susceptibility. Potato virus Y (PVY) is found worldwide, and is one of the main virus problems for potato growers. PVY is transmitted by aphids and mechanically by machinery, tools and people, and symptoms are variable across cultivars and strains, including being symptomless in some cultivars. Therefore, breeding resistant cultivars is the best way to control this virus. This study phenotypically screened 74 of the main commercial cultivars and a few other select cultivars grown in Australia, in order to identify sources of resistance to PVY. The cultivars were screened against PVYO and PVYNTN, with 23 out of 71 resistant to PVYO and 13 out of 74 resistant to PVYNTN, and all these 13 were resistant to both strains. When the phenotypic screening was compared to the results listed on the European Cultivated Potato Database, the majority of results were found to be consistent. We then evaluated three molecular markers RYSC3, M45, and STM0003 for the extreme resistance genes Ryadg and Rysto, to validate the usefulness of the markers for marker-assisted selection (MAS) on Australian germplasm. The degree of correlation between the resistance phenotypes and the RYSC3, M45, and STM0003 markers for Ryadg and Rysto conferred PVY resistance was determined. Three cultivars amplified the RYSC3 marker, while the M45 marker amplified the same 3 and an additional 9. Of the 12 cultivars, 11 phenotyped as resistant, but 1 was susceptible. The STM0003 marker was amplified from only 2 cultivars that both had resistant phenotypes. The RYSC3, M45, and STM0003 markers were therefore able to identify all the 13 cultivars that were resistant to both strains of PVY. Therefore, these markers will enable the identification of genotypes with resistance to PVY, and enable PVY resistant parents to be used for the development of superior progeny; these genetic markers can be used for MAS in the Australian potato breeding program.

scholarly journals Detection of molecular markers linked to Ry genes in potato germplasm for marker-assisted selection for extreme resistance to PVY in AAFC’s potato breeding program

2016 ◽  
Vol 96 (5) ◽  
pp. 737-742 ◽  
Author(s):  
Xianzhou Nie ◽  
Fayruza Lalany ◽  
Virginia Dickison ◽  
Donna Wilson ◽  
Mathuresh Singh ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Potato Virus ◽  
Marker Assisted Selection ◽  
Potato Breeding ◽  
Potato Virus Y ◽  
Breeding Program ◽  
Potato Breeding Program ◽  
Extreme Resistance ◽  
Potato Germplasm ◽  
Selection For

Molecular markers reported to be linked to extreme resistance (ER) against Potato virus Y (PVY) were evaluated in potato germplasm. YES3-3A and YES3-3B, markers linked to Rysto, were detected in ‘Barbara’ and its three descendants that exhibit ER to PVY; RYSC3, a marker linked to Ryadg, was detected in breeding clones NY121 and NY123. Assessment of RYSC3 as a marker for selection for Ryadg-mediated ER validated its efficacy in identification of selections with ER to PVY.

scholarly journals Resistance of potato clones to necrotic recombinant strains of potato virus y (pvy)

2014 ◽  
Vol 38 (4) ◽  
pp. 343-351 ◽  
Author(s):  
Silvia Regina Rodrigues de Paula Ribeiro ◽  
César Augusto Brasil Pereira Pinto ◽  
Suellen Bárbara Ferreira Galvino Costa ◽  
Mariney de Menezes ◽  
Antonia dos Reis Figueira
Keyword(s):  
Molecular Marker ◽  
Potato Virus ◽  
Potato Breeding ◽  
Potato Virus Y ◽  
Resistance Allele ◽  
Potato Breeding Program ◽  
Extreme Resistance ◽  
Tobacco Plants ◽  
Resistant Allele ◽  
Recombinant Strains

The Ry adg allele is widely used by breeders to confer extreme resistance to all strains of PVY. However, the necrotic strain has increased recombination resulting in recent considerable losses in productivity. Thus far, not all necrotic recombinant strains of PVY have been tested for their reaction to the Ry gene. The objective of this study was to identify potato clones carrying the resistant allele and to assess their reaction to the following recombinant strains: NTN (PVY NTN), Wilga (PVY N-Wi), and "curly top" (PVY E). Advanced clones from the potato breeding program at Universidade Federal de Lavras were evaluated through a specific molecular marker for the Ry adg allele. The clones carrying the resistance allele were grafted on tobacco plants infected with necrotic recombinant strains of PVY. The clones carrying the allele for resistance to PVY were not infected with any of the recombinants during the grafting test. These results confirm that resistance to necrotic recombinant strains has not yet been overcome and that the Ry adg allele also confers resistance to the three recombinant strains tested.

Validation and Implementation of Marker-Assisted Selection (MAS) for PVY Resistance (Ry adg gene) in a Tetraploid Potato Breeding Program

2009 ◽  
Vol 86 (4) ◽  
pp. 304-314 ◽  
Author(s):  
Ryon J. Ottoman ◽  
Dan C. Hane ◽  
Charles R. Brown ◽  
Solomon Yilma ◽  
Steven R. James ◽  
...  
Keyword(s):  
Marker Assisted Selection ◽  
Potato Breeding ◽  
Breeding Program ◽  
Potato Breeding Program ◽  
Tetraploid Potato ◽  
Pvy Resistance

scholarly journals Strain-Specific Hypersensitive and Extreme Resistance Phenotypes Elicited by Potato virus Y Among 39 Potato Cultivars Released in Three World Regions Over a 117-Year Period

Plant Disease ◽  
2018 ◽  
Vol 102 (1) ◽  
pp. 185-196 ◽  
Author(s):  
Roger A. C. Jones ◽  
Stuart J. Vincent
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Secondary Infection ◽  
Potato Cultivars ◽  
Symptom Expression ◽  
Extreme Resistance ◽  
Potato Plants ◽  
Pvy Resistance ◽  
World Regions ◽  
Healthy Seed

Strain-specific hypersensitive (HR) and extreme resistance (ER) phenotypes elicited in potato plants by three Potato virus Y (PVY) isolates in strain groups PVYO (BL and DEL3) and PVYD (KIP1) were studied. PVYO and PVYD isolates elicit HR genes Ny or putative Nd, respectively, and all three isolates elicit ER gene Ry. They were inoculated to 39 Australasian, European, or North American potato cultivars released over a 117-year period and harvested tubers were replanted. Both primary and secondary symptoms were recorded. Two European cultivars always developed ER following sap and graft inoculation and, thus, carried comprehensive PVY resistance gene Ry. One Australasian and two European cultivars always developed susceptible phenotypes and, thus, lacked genes Ry, Ny, and putative Nd. Sap inoculation with isolate KIP1 elicited localized HR (LHR) in 31 cultivars and both LHR and systemic HR (SHR) in three others; thus, all carried putative Nd. Isolates BL and DEL3 both elicited susceptible phenotypes in 11 of these 34 cultivars but LHR alone, SHR alone, or both LHR and SHR in the other 23 which, therefore, all carry Ny. With these two isolates, SHR expression ranged from very severe to very weak, with the greatest numbers of isolate–cultivar combinations occurring in the severe category with BL (n = 11) and moderate category (n = 12) with DEL3. Within the same isolate–cultivar combination, overall, SHR symptom expression was weaker with secondary than primary infection. With both primary and secondary infection, SHR expression was most severe with KIP1 and weakest with DEL3. Genes Ny and putative Nd were present in cultivars released between 1939 and 2010 or 1893 and 2010, respectively, occurring in cultivars from all three world regions. These findings have important implications concerning breeding new PVY-resistant potato cultivars, especially for countries lacking healthy seed potato stocks, or where subsistence farmers cannot afford them. An alternative to including gene Ry is incorporating as many strain-specific PVY resistance genes as possible.

Sign in / Sign up

Export Citation Format

Share Document