limiting factors
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2022 ◽  
Vol 23 (2) ◽  
pp. 957
Franziska Steger ◽  
Johanna Reich ◽  
Werner Fuchs ◽  
Simon K.-M. R. Rittmann ◽  
Georg M. Gübitz ◽  

Strategies for depleting carbon dioxide (CO2) from flue gases are urgently needed and carbonic anhydrases (CAs) can contribute to solving this problem. They catalyze the hydration of CO2 in aqueous solutions and therefore capture the CO2. However, the harsh conditions due to varying process temperatures are limiting factors for the application of enzymes. The current study aims to examine four recombinantly produced CAs from different organisms, namely CAs from Acetobacterium woodii (AwCA or CynT), Persephonella marina (PmCA), Methanobacterium thermoautotrophicum (MtaCA or Cab) and Sulphurihydrogenibium yellowstonense (SspCA). The highest expression yields and activities were found for AwCA (1814 WAU mg−1 AwCA) and PmCA (1748 WAU mg−1 PmCA). AwCA was highly stable in a mesophilic temperature range, whereas PmCA proved to be exceptionally thermostable. Our results indicate the potential to utilize CAs from anaerobic microorganisms to develop CO2 sequestration applications.

2022 ◽  
Aguilar Carrasco María José ◽  
Gielen Eric ◽  
Vallés Planells María Concepción ◽  
Galiana Galán Francisco ◽  
Riutort Mayol Gabriel ◽  

2022 ◽  
Anshuka Anshuka ◽  
Alexander JV Buzacott ◽  
Floris van Ogtrop

Abstract Monitoring hydrological extremes is essential for developing risk-mitigation strategies. One of the limiting factors for this is the absence of reliable on the ground monitoring networks that capture data on climate variables, which is highly evident in developing states such as Fiji. Fortunately, increasing global coverage of satellite-derived datasets is facilitating utilisation of this information for monitoring dry and wet periods in data sparse regions. In this study, three global satellite rainfall datasets (CHIRPS, PERSIANN-CDR and CPC) were evaluated for Fiji. All satellite products had reasonable correlations with station data, and CPC had the highest correlation with minimum error values. The Effective Drought Index (EDI), a useful index for understanding hydrological extremes, was then calculated. Thereafter, a canonical correlation analysis (CCA) was employed to forecast the EDI using sea surface temperature anomaly (SSTa) data. A high canonical correlation of 0.98 was achieved between the PCs of mean SST and mean EDI, showing the influence of ocean–atmospheric interactions on precipitation regimes in Fiji. CCA was used to perform a hind cast and a short-term forecast. The training stage produced a coefficient of determinant (R2) value of 0.83 and mean square error (MSE) of 0.11. The results in the testing stage for the forecast were more modest, with an R2 of 0.45 and MSE of 0.26. This easy-to-implement system can be a useful tool used by disaster management bodies to aid in enacting water restrictions, providing aid, and making informed agronomic decisions such as planting dates or extents.

Pharmacy ◽  
2022 ◽  
Vol 10 (1) ◽  
pp. 15
Selina Taylor ◽  
Alice Cairns ◽  
Beverley Glass

Aim: The aim of this study is to explore pharmacist perspectives of the implementation of a community pharmacy-based ear health service in rural communities. Method: A community pharmacy-based health service model was designed and developed to provide an accessible ear care service (LISTEN UP—Locally Integrated Screening and Testing Ear aNd aUral Program) and pharmacist’s perspectives of the implementation of LISTEN UP were explored. Thematic analysis was conducted and data coded according to the Consolidated Framework for Implementation Research. Results: A total of 20 interviews were conducted with 10 pharmacists, averaging 30 min. Visualistion of the ear canal was reported as the greatest advantage of the service, whilst the time required for documentation reported as a complexity. The number of pharmacists working at one time and the availability of a private consultation room were identified as the two limiting factors for execution. On reflection, the need for government funding for service viability and sustainability was highlighted. Discussion/Conclusion: Expanded pharmacy practice is emerging for the Australian pharmacy profession. Rural community pharmacists are recognised as integral members of healthcare teams, providing accessible medication supply and health advice to seven million people in Australia who call rural and remote regions home. However, there are no structured models supporting them to provide expanded services to improve health outcomes in their communities. This study provides lessons learnt to guide future design and development of expanded models of pharmacy practice.

2022 ◽  
Vol 1 ◽  
pp. 103
Camilla Bertolini ◽  
Roberto Pastres

Background: Active restoration is necessary to enhance the recovery of Ostrea edulis reefs, which contribute to many ecosystem services. Restoration can be integrated within aquaculture practices, bringing positive environmental changes while maximising space utilisation. The restoration project MAREA (MAtchmaking Restoration Ecology and Aquaculture) aims to bring back O. edulis in the North-West Adriatic addressing the feasibility of its cultivation. Both successful restoration and sustainable aquaculture require a thorough understanding of the ecological needs, as the requirements of both activities need to be harmonized. Therefore, one of the preliminary activities before embarking on the pilot was the completion of a thorough literature review to identify research directions and gaps required for ‘restorative aquaculture’, aiming to gather the most up to date O. edulis knowledge on a global and local scale.  Methods: Internet (Web of Science, Scopus, Google scholar) and physical resources (libraries) were searched for all available global and local knowledge on O. edulis. Bibliometrix was used to identify the main research topics using keywords, titles, and abstracts analyses. Studies were then manually screened and summarised to extract knowledge specific to restoration and aquaculture. Results: While restoration studies are recent, evidence for the loss of this species and potential causes (and solutions) have been discussed since the end of the 19th century. While diseases were a leading cause for reef loss, substratum limitation appears to be one of the leading limiting factors for both restoration and aquaculture of O. edulis, and was already mentioned in the early texts that were found. Conclusions: The review highlighted that restoration success and aquaculture feasibility depend upon the crucial stage of settlement. The project ‘MAREA’ will therefore increase its focus on this stage, both in terms of timing, location, and materials for settlement plates placement.

2022 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Kerri M. Camp ◽  
Marilyn Young ◽  
Stephen C. Bushardt

Purpose This paper aims to propose that millennials – those born between 1980 and 1995 – are uniquely impacted by the long-term impact of the pandemic, which has accelerated the work from home movement and exacerbated organizational issues associated with working remotely. Millennials, on the cusp of embarking on important leadership roles in this new remote work environment, pose challenges and opportunities for organizations will that will last long after the pandemic. Design/methodology/approach This paper provides a viewpoint based on a narrative review and on the authors’ professional experiences within organizations. Based upon these findings, this paper has reimagined the Mumford skills model to explore millennial managers in this new remote work, post-pandemic context. The goal of this narrative review was to provide a full picture of the “Millennial manager” and the challenges they face in becoming effective managers, as well as highlight the important strengths they bring to the table. Findings Managers may have challenges regarding job satisfaction, productivity and organizational commitment. The positive factors are millennials prefer flexibility, teamwork and creating a positive work-life balance. The negative side has to do with isolation and the ability to embrace the organization culture in a remote environment. This model shows positive and limiting factors of millennials related to organization effectiveness. The major propositions and model were that millennium managers in this new environment increase interpersonal communication to maintain trust, effective mentoring, resolve strong organizational culture and ensure effective delegation and conflict resolution. Practical implications This study discovered challenges for managers, including developing loyalty and improving employee job satisfaction and organizational commitment. Active listening is necessary for team management to show that all team members are valued irrespective of the work environment. Active listening and empathy will increase social support at work, which improves personal well-being and productivity. Millennials will continue to use their technological skills, their desire for teamwork and their preference for participative management, resulting in becoming an invaluable asset in this era of organizational transformation. The challenge for organizations is to realize the millennial generation possesses many talents and must successfully engage them in the pursuit of organizational goals. Originality/value This paper adds to the body of knowledge regarding millennials in organizations with a specific focus on the new work environment created by the global pandemic. The authors hope that their adapted skills model – the millennial manager skills model – becomes an important resource for articulation this new environment that millennial managers are in and that this model can be further refined and expanded through empirical exploration.

Animals ◽  
2022 ◽  
Vol 12 (2) ◽  
pp. 153
Miguel A. Gutiérrez-Reinoso ◽  
Constanza J. Aguilera ◽  
Felipe Navarrete ◽  
Joel Cabezas ◽  
Fidel O. Castro ◽  

Over the last few years, several commercial FSH products have been developed for cattle superovulation (SOV) purposes in Multiple Ovulation and Embryo Transfer (MOET) programs. The SOV response is highly variable among individuals and remains one of the main limiting factors in obtaining a profitable number of transferable embryos. In this study, follicle stimulating hormone (FSH) from different origins was included in two SOV protocols, (a) FSH from purified pig pituitary extract (NIH-FSH-p; two doses/day, 12 h apart, four consecutive days); and (b) extra-long-acting bovine recombinant FSH (bscrFSH; a single dose/day, four consecutive days), to test the effects of bscrFSH on the ovarian response, hormone profile levels, in vivo embryo production and the pluripotency gene expression of the obtained embryos. A total of 68 healthy primiparous red Angus cows (Bos taurus) were randomly distributed into two experimental groups (n = 34 each). Blood sample collection for progesterone (P4) and cortisol (C) level determination was performed together with ultrasonographic assessment for ovarian size, follicles (FL) and corpora lutea (CL) quantification in each SOV protocol (Day 0, 4, 8, and 15). Moreover, FSH profiles were monitorised throughout both protocols (Day 0, 4, 5, 6, 7, 8, 9, 10, and 15). In vivo embryo quantity and quality (total structures, morulae, blastocysts, viable, degenerated and blocked embryos) were recorded in each SOV protocol. Finally, embryo quality in both protocols was assessed by the analysis of the expression level of crucial genes for early embryo development (OCT4, IFNt, CDX2, BCL2, and BAX). P4 and cortisol concentration peaks in both SOV protocols were obtained on Day 15 and Day 8, respectively, which were statistically different compared to the other time-points (p < 0.05). Ovarian dimensions increased from Day 0 to Day 15 irrespective of the SOV protocol considered (p < 0.05). Significant changes in CL number were observed over time till Day 15 irrespective of the SOV protocol applied (p < 0.05), being non- significantly different between SOV protocols within each time-point (p > 0.05). The number of CL was higher on Day 15 in the bscrFSH group compared to the NIH-FSH-p group (p < 0.05). The number of embryonic structures recovered was higher in the bscrFSH group (p = 0.025), probably as a result of a tendency towards a greater number of follicles developed compared to the NIH-FSH-p group. IFNt and BAX were overexpressed in embryos from the bscrFSH group (p < 0.05), with a fold change of 16 and 1.3, respectively. However, no statistical differences were detected regarding the OCT4, CDX2, BCL2, and BCL2/BAX expression ratio (p > 0.05). In conclusion, including bscrFSH in SOV protocols could be an important alternative by reducing the number of applications and offering an improved ovarian response together with better embryo quality and superior performance in embryo production compared to NIH-FSH-p SOV protocols.

Medicina ◽  
2022 ◽  
Vol 58 (1) ◽  
pp. 99
Ruud Droeghaag ◽  
Inge J. M. H. Caelers ◽  
Aggie T. G. Paulus ◽  
Wouter L. W. van Hemert ◽  
Henk van Santbrink ◽  

Background and Objectives: Only limited qualitative research concerning instrumented spine surgeries has been published, despite the increasing number of these surgeries and the evident importance of qualitative analysis of the processes surrounding these complex interventions. Current qualitative research is mainly limited to the experiences, emotions and expectations of patients. Insight into the full process, including experiences from the perspective of informal caregivers and healthcare professionals, remains scarce. Materials and Methods: Data were gathered by means of semi-structured face-to-face interviews. In total, there were 27 participants, including 11 patients, 7 informal caregivers and 9 healthcare professionals. The interview process was audiotaped, and each interview was transcribed verbatim. To systematically analyse the gathered data, software for qualitative analysis (NVivo) was used. After immersion in the raw data of transcripts and field notes, a list of broad categories for organising the data into meaningful clusters for analysis was developed. All interviews were coded by the first author, and 25% was independently assessed by the second author. Results: The results of our study describe several promoting and limiting factors concerning the process of lumbar fusion surgery from the perspective of patients, informal caregivers and healthcare providers. The most frequently mentioned promoting factors were: information and opportunities to ask questions during consultations; multidisciplinary consultations; good communication and guidance during hospitalization; and follow-up appointments. The most frequently mentioned limiting factors were: lack of educational material; lack of guidance and communication prior to, during and after hospitalisation. Conclusion: Overall, participants were satisfied with the current healthcare-process in lumbar fusion surgery. However, we found that lack of educational material and guidance during the process led to insecurity about complaints, surgery and recovery. To improve the process of lumbar interbody fusion and to increase patient satisfaction, healthcare providers should focus on guiding and educating patients and informal caregivers about the pre-operative trajectory, the surgery and the recovery. From the healthcare providers’ perspective, the process could be improved by multidisciplinary consultations and a dedicated spine team in the operation room. Although this study focusses on lumbar fusion surgery, results could be translated to other fields of spine surgery and surgery in general.

2022 ◽  
Vol 163 (2) ◽  
pp. 44
Bradley M. S. Hansen

Abstract We present a catalog of unbound stellar pairs, within 100 pc of the Sun, that are undergoing close, hyperbolic, encounters. The data are drawn from the GAIA EDR3 catalog, and the limiting factors are errors in the radial distance and unknown velocities along the line of sight. Such stellar pairs have been suggested to be possible events associated with the migration of technological civilizations between stars. As such, this sample may represent a finite set of targets for a SETI search based on this hypothesis. Our catalog contains a total of 132 close passage events, featuring stars from across the entire main sequence, with 16 pairs featuring at least one main-sequence star of spectral type between K1 and F3. Many of these stars are also in binaries, so that we isolate eight single stars as the most likely candidates to search for an ongoing migration event—HD 87978, HD 92577, HD 50669, HD 44006, HD 80790, LSPM J2126+5338, LSPM J0646+1829 and HD 192486. Among host stars of known planets, the stars GJ 433 and HR 858 are the best candidates.

A. Krisnawati ◽  
K. Noerwijati ◽  
S.W. Indiati ◽  
Trustinah . ◽  
E. Yusnawan ◽  

Background: The infestation of the pod sucking bug Riptortus linearis is one of the limiting factors of soybean productivity in Indonesia. The research aims were to identify the level of resistance to the pod sucking bug R. linearis on several soybean genotypes based on the no-choice test (NCT) and free-choice tests (FCT). Methods: The genetic materials used were 49 soybean genotypes. The study was conducted in the Indonesian Legumes and Tuber Crops Research Institute (ILETRI) from August to December 2020, using the randomized block design in triplicates. The evaluation for pod sucking bug resistance was based on NCT and FCT. Result: The FCT resulted in two genotypes with a consistent moderately resistance level (20-40% of damage intensity) to pod sucking bug based on the pod and seed damage intensity. The NCT method resulted in five genotypes as moderately resistant (20-40% of damage intensity) to pod sucking bug based on the seed damage. The NCT resulted in higher average intensity of pod and seed damage (80.25% and 71.23%, respectively) than the FCT (69.91% and 69.09%, respectively). Two soybean genotypes (Degra/Anjasmoro-1-559 and Anjasmoro/IAC100-2-618) with a consistent moderately resistance level could be used for cultivar improvement in the breeding program. The pod trichome density was suggested to be one of the effective morphological defenses against the pod sucking bug attack.

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