scholarly journals Seasonal distribution of the shellfish-killing dinoflagellate Heterocapsa circularisquama in Ago Bay monitored by an indirect fluorescent antibody technique using monoclonal antibodies

2007 ◽  
Vol 2 (1) ◽  
pp. 49-62 ◽  
Author(s):  
TOMOTAKA SHIRAISHI ◽  
SHINGO HIROISHI ◽  
KIYOHITO NAGAI ◽  
JYOJI GO ◽  
TAKASHI YAMAMOTO ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Fluorescent Antibody ◽  
Seasonal Distribution ◽  
Fluorescent Antibody Technique ◽  
Indirect Fluorescent Antibody ◽  
Antibody Technique ◽  
Heterocapsa Circularisquama

Rapid quantification and in situ detection of nitrifying bacteria in biofilms by monoclonal antibody method

2000 ◽  
Vol 41 (4-5) ◽  
pp. 301-308 ◽  
Author(s):  
N. Noda ◽  
H. Ikuta ◽  
Y. Ebie ◽  
A. Hirata ◽  
S. Tsuneda ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Fluorescent Antibody ◽  
Nitrifying Bacteria ◽  
Fluorescent Antibody Technique ◽  
Antibody Technique ◽  
Antibody Method ◽  
In Situ Detection ◽  
Rapid Quantification

Fluorescent antibody technique by the monoclonal antibody method is very useful and helpful for the rapid quantification and in situ detection of the specific bacteria like nitrifiers in a mixed baxterial habitat such as a biofilm. In this study, twelve monoclonal antibodies against Nitrosomonas europaea (IFO14298) and sixteen against Nitrobacter winogradskyi (IFO14297) were raised from splenocytes of mice (BALB/c). It was found that these antibodies exhibited little cross reactivity against various kinds of heterotrophic bacteria. The direct cell count method using monoclonal antibodies could exactly detect and rapidly quantify N. europaea and N. winogradskyi. Moreover, the distribution of N. europaea and N. winogradskyi in a biofilm could be examined by in situ fluorescent antibody technique. It was shown that most of N. winogradskyi existed near the surface part and most of N. europaea existed at the inner part of the polyethylene glycol (PEG) gel pellet, which had entrapped activated sludge and used in a landfill leachate treatment reactor. It was suggested that this monoclonal antibody method was utilized for estimating and controlling the population of nitrifying bacteria as a quick and favorable tool.

Rubella Antibodies in Human Serum: Detection by the Indirect Fluorescent-Antibody Technique

Science ◽  
1964 ◽  
Vol 145 (3635) ◽  
pp. 943-945 ◽  
Author(s):  
G. C. Brown ◽  
H. F. Maassab ◽  
J. A. Veronelli ◽  
T. J. Francis
Keyword(s):  
Human Serum ◽  
Fluorescent Antibody ◽  
Fluorescent Antibody Technique ◽  
Indirect Fluorescent Antibody ◽  
Antibody Technique ◽  
Serum Detection

scholarly journals Toxoplasma antibodies in immigrants from Hong Kong

1974 ◽  
Vol 72 (1) ◽  
pp. 19-22 ◽  
Author(s):  
S. K. K. Seah
Keyword(s):  
Hong Kong ◽  
Toxoplasma Gondii ◽  
Indigenous Population ◽  
Fluorescent Antibody ◽  
Age Groups ◽  
Fluorescent Antibody Technique ◽  
Indirect Fluorescent Antibody ◽  
Chinese Adults ◽  
Antibody Technique ◽  
Total Incidence

SUMMARYThe sera of 200 Chinese adults recently immigrated to Canada from Hong Kong were examined for antibodies to Toxoplasma gondii by the indirect fluorescent antibody technique. It was found that the total incidence was 18·0%. This is significantly lower than that of the indigenous population of the same age groups in this area.

scholarly journals Diagnosis of Avian Infectious Bronchitis by Indirect Fluorescent Antibody Technique Using Monoclonal Antibody

1993 ◽  
Vol 46 (2) ◽  
pp. 117-119 ◽  
Author(s):  
Kazuki ISHIBASHI ◽  
Hitomi SHIRAKAWA ◽  
Yoshifumi TOMISHITA ◽  
Hiroyuki MATUO ◽  
Akira WATANABE ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Fluorescent Antibody ◽  
Fluorescent Antibody Technique ◽  
Indirect Fluorescent Antibody ◽  
Antibody Technique ◽  
Infectious Bronchitis

scholarly journals Fluorescent antibody test for the serological diagnosis of gonorrhea

1976 ◽  
Vol 3 (4) ◽  
pp. 438-442
Author(s):  
H A Gaafar ◽  
D C D'Arcangelis
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Immunoglobulin G ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Serological Diagnosis ◽  
Fluorescent Antibody Technique ◽  
Indirect Fluorescent Antibody ◽  
Antibody Technique ◽  
Normal Individuals ◽  
Male Patients

An indirect fluorescent antibody technique has been developed for the serological diagnosis of gonorrhea. The selected strain(s) of Neisseria gonorrhoeae possesses a heat-labile surface antigen (L-antigen). Sera are diluted 1:10, and an aliquot is hear inactivated at 59 C for 30 min. The treated and untreated aliquots are then examined for human immunoglobulin G anti-L-antigen. In a prelimiary study of 495 sera, 95% of those from women with a bacteriologically confirmed diagnosis of gorrhea and 87% of those from male patients were reactive in this test, whereas only 1.4% fo the sera from presumably normal individuals were reactive.

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