Purification and Characterization of a Chitinolytic Enzyme Produced by Bacillus licheniformis GA9

2016 ◽  
Vol 44 (4) ◽  
pp. 470-478
Author(s):  
Dong Ho Hwang ◽  
Sung Wook Hong ◽  
Hyung seo Hwang ◽  
Kun Sub Chung
Keyword(s):  
Bacillus Licheniformis ◽  
Purification And Characterization ◽  
Chitinolytic Enzyme

Purification and characterization of an exochitinase from Bacillus thuringiensis subsp. aizawai and its action against phytopathogenic fungi

2006 ◽  
Vol 52 (7) ◽  
pp. 651-657 ◽  
Author(s):  
Luis Morales de la Vega ◽  
J Eleazar Barboza-Corona ◽  
Maria G Aguilar-Uscanga ◽  
Mario Ramírez-Lepe
Keyword(s):  
Bacillus Thuringiensis ◽  
Sodium Dodecyl ◽  
Sclerotium Rolfsii ◽  
Phytopathogenic Fungi ◽  
Purification And Characterization ◽  
Chitinolytic Enzyme ◽  
Bacillus Thuringiensis Subsp ◽  
Sds Page ◽  
Fusarium Sp

A chitinolytic enzyme from Bacillus thuringiensis subsp. aizawai has been purified and its molecular mass was estimated ca. 66 kDa by sodium dodecyl sulfate – polyacryamide gel electrophoresis (SDS–PAGE). The enzyme was able to hydrolyze chitin to chitobiosides but not carboxymethylcellulose, cellulose, pullulan, and laminarin. Optimal pH and temperature were detected at 6 and 50 °C, respectively. Stability, in the absence of substrate, was observed at temperatures less than 60 °C and pH between 5 and 8. Enzyme activity was significantly inhibited by K+ and EDTA and completely inhibited by Hg2+. Purified chitinase showed lytic activity against cell walls from six phytopathogenic fungi and inhibited the mycelial growth of both Fusarium sp. and Sclerotium rolfsii. The biocontrol efficacy of the enzyme was tested in the protection of bean seeds infested with six phytopathogenic fungi.Key words: chitinase, Bacillus thuringiensis, purification, phytopathogenic fungi.

Purification and characterization of a peptide from Bacillus licheniformis showing dual antimicrobial and emulsifying activities

2009 ◽  
Vol 42 (1) ◽  
pp. 63-68 ◽  
Author(s):  
Mário Lettieri Teixeira ◽  
Florencia Cladera-Olivera ◽  
Juliana dos Santos ◽  
Adriano Brandelli
Keyword(s):  
Bacillus Licheniformis ◽  
Purification And Characterization

Cloning, purification, and characterization of β-galactosidase from Bacillus licheniformis DSM 13

2010 ◽  
Vol 89 (3) ◽  
pp. 645-654 ◽  
Author(s):  
Onladda Juajun ◽  
Thu-Ha Nguyen ◽  
Thomas Maischberger ◽  
Sanaullah Iqbal ◽  
Dietmar Haltrich ◽  
...  
Keyword(s):  
Bacillus Licheniformis ◽  
Purification And Characterization

Purification and Characterization of Two Xylanases From Alkalophilic and Thermophilic Bacillus licheniformis 77-2

2006 ◽  
Vol 129 (1-3) ◽  
pp. 289-302 ◽  
Author(s):  
Valquiria B. Damiano ◽  
Richard Ward ◽  
Eleni Gomes ◽  
Heloiza Ferreira Alves-Prado ◽  
Roberto DA Silva
Keyword(s):  
Bacillus Licheniformis ◽  
Purification And Characterization ◽  
Thermophilic Bacillus

Purification and characterization of a halotolerant serine proteinase from thermotolerant Bacillus licheniformis RKK-04 isolated from Thai fish sauce

2010 ◽  
Vol 86 (6) ◽  
pp. 1867-1875 ◽  
Author(s):  
Yoichi Toyokawa ◽  
Hiroaki Takahara ◽  
Alissara Reungsang ◽  
Masakazu Fukuta ◽  
Yuki Hachimine ◽  
...  
Keyword(s):  
Bacillus Licheniformis ◽  
Serine Proteinase ◽  
Fish Sauce ◽  
Purification And Characterization

Purification and characterization of antimicrobial substances from Bacillus licheniformis BFP011

2014 ◽  
Vol 50 (6) ◽  
pp. 580-587 ◽  
Author(s):  
N. Arbsuwan ◽  
P. Sirithorn ◽  
S. Daduang ◽  
A. Dhiravisit ◽  
S. Thammasirirak
Keyword(s):  
Bacillus Licheniformis ◽  
Purification And Characterization ◽  
Antimicrobial Substances

Purification and characterization of a thermostable alpha-amylase from Bacillus licheniformis

1993 ◽  
Vol 28 (2-3) ◽  
pp. 277-289 ◽  
Author(s):  
Viara N. Ivanova ◽  
Elena P. Dobreva ◽  
Elka I. Emanuilova
Keyword(s):  
Bacillus Licheniformis ◽  
Alpha Amylase ◽  
Purification And Characterization
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