The Chronic Toxic Effect of Water-Accommodated Fractions of 0# Diesel Oil on Splox Gene of Sea Urchin

0# diesel is common petroleum hydrocarbon pollutant of sea and marine organisms. One goal of this study was to assess the chronic toxic effect of water-accommodated fractions（WAFS）of 0# diesel oil on Splox gene of Strongylocentyotus intermedius by PCR-SSCP. The experiment was carried out with three concentrations (5mg/L, 20mg/L and 50mg/l). The results show that with the increase of exposure concentration and time, the mutation probability of the sea urchin increased. In the four primers, single base mutation took place in coding region of the 41 of Primer A-1, with the conversion of A-G, where amino acid changed from lysine into arginine.