Purification and biochemical characterization of alkaline protease from an Egyptian biopesticide-producing Bacillus sphaericus strain

2011 ◽  
Vol 5 (28) ◽  
Author(s):  
Mourad A. M. Aboul-Soud
Keyword(s):  
Alkaline Protease ◽  
Biochemical Characterization ◽  
Bacillus Sphaericus

scholarly journals Cloning and Characterization of the Bile Salt Hydrolase Genes (bsh) from Bifidobacterium bifidum Strains

2004 ◽  
Vol 70 (9) ◽  
pp. 5603-5612 ◽  
Author(s):  
Geun-Bae Kim ◽  
Carol M. Miyamoto ◽  
Edward A. Meighen ◽  
Byong H. Lee
Keyword(s):  
Bile Salt ◽  
Active Sites ◽  
Biochemical Characterization ◽  
Bifidobacterium Longum ◽  
Pcr Amplification ◽  
Bacillus Sphaericus ◽  
Bifidobacterium Bifidum ◽  
Bile Salt Hydrolase ◽  
Cloned Gene

ABSTRACT Biochemical characterization of the purified bile salt hydrolase (BSH) from Bifidobacterium bifidum ATCC 11863 revealed some distinct characteristics not observed in other species of Bifidobacterium. The bsh gene was cloned from B. bifidum, and the DNA flanking the bsh gene was sequenced. Comparison of the deduced amino acid sequence of the cloned gene with previously known sequences revealed high homology with BSH enzymes from several microorganisms and penicillin V amidase (PVA) of Bacillus sphaericus. The proposed active sites of PVA were highly conserved, including that of the Cys-1 residue. The importance of the SH group in the N-terminal cysteine was confirmed by substitution of Cys with chemically and structurally similar residues, Ser or Thr, both of which resulted in an inactive enzyme. The transcriptional start point of the bsh gene has been determined by primer extension analysis. Unlike Bifidobacterium longum bsh, B. bifidum bsh was transcribed as a monocistronic unit, which was confirmed by Northern blot analysis. PCR amplification with the type-specific primer set revealed the high level of sequence homology in their bsh genes within the species of B. bifidum.

Biochemical characterization of a detergent-stable serine alkaline protease from Caldicoprobacter guelmensis

2015 ◽  
Vol 81 ◽  
pp. 299-307 ◽  
Author(s):  
Khelifa Bouacem ◽  
Amel Bouanane-Darenfed ◽  
Hassiba Laribi-Habchi ◽  
Mouna Ben Elhoul ◽  
Aïda Hmida-Sayari ◽  
...  
Keyword(s):  
Alkaline Protease ◽  
Biochemical Characterization ◽  
Serine Alkaline Protease

Purification and biochemical characterization of an alkaline protease from marine bacteriaPseudoalteromonassp. 129-1

2015 ◽  
Vol 55 (12) ◽  
pp. 1427-1434 ◽  
Author(s):  
Shimei Wu ◽  
Ge Liu ◽  
Dechao Zhang ◽  
Chaoxu Li ◽  
Chaomin Sun
Keyword(s):  
Alkaline Protease ◽  
Biochemical Characterization
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