Self-healing mortar using different types, content, and concentrations of bacteria to repair cracks.

The creation of cracks, which are the most common cause of structural failure, has a significant impact on the structure's strength and durability. As a result, effective repair and maintenance are vital and unavoidable for treating any of these issues. Self-healing mortar holds promising benefits for reducing the cost of repair as cracks are autonomously repaired without any human intervention. This study investigated the effect of bacteria type, bacteria content, bacteria concentration, and nutrient type on the properties of the self-healing mortar. Three types of bacteria, Bacillus sphaericus, Bacillus Megaterium, and Bacillus subtilis encapsulated in calcium alginate beads, were introduced into the mortar. Two concentrations of bacteria, 2× 108 and 2× 109 Colony Forming Units per milliliter, and different percentages of bacteria of cement weight were selected for the study. In addition, calcium lactate and calcium acetate were used at 0.5% of cement weight as nutrition for bacteria. Tests were performed for compressive strength, bending strength, SEM, EDX, and TGA/DTG. The results show a significant development in the mechanical behaviour of mortar, especially with Bacillus Megaterium using a 2.5% bacterial proportion with a concentration 2× 109 CFU/ml. This can be related to the filling of voids and cracks in microbial mortar by calcite, which was confirmed by SEM and EDX.

Use of Bacteria Externally for Repairing Cracks and Improving Properties of Concrete Exposed to High Temperatures

The current paper presents the results of an experimental study on the application of calcium carbonate precipitation bacteria as a new approach to repairing damaged concrete when exposed to high temperatures. To do so, cylindrical and cubic concrete specimens were initially exposed to heat in a furnace for 1 h, after reaching two different temperatures of 600 and 800 °C. A heat rate of 5.5 °C per minute was used to achieve the target temperatures. Then, two types of bacteria, namely Sporosarcina pasteurii and Bacillus sphaericus, with cell concentration of 107 cells/mL, were utilized externally, to repair the thermal cracks, enhancing the mechanical properties and durability of the damaged concrete. The efficiency of the bacterial remediation technique was then evaluated through compressive strength, ultrasonic pulse velocity (UPV), and electrical conductivity tests on the control specimens (unexposed to heat), and those exposed to high temperature with or without bacterial healing. The experimental results demonstrate that the compressive strength of the test specimens exposed to temperatures of 600 and 800 °C decreased by about 31–44% compared with the control ones. However, compared to those damaged at 600 and 800 °C, the compressive strength of specimens repaired by the S. pasteurii and the B. sphaericus showed increases of 31–93%. This increase is associated with the precipitation of calcium carbonate in the deep and superficial cracks and pores of the damaged specimens. Furthermore, the ultrasonic pulse velocity of the specimens subjected to bacterial remediation had a significant increase of about 1.65–3.47 times compared with the damaged ones. In addition, the electrical conductivity of repaired specimens decreased by 22–36% compared with the damaged specimens.

Reliability of Unconventional Concrete: Improvement in Mix Design and Addition of Bacterial Admixture

Portland cement is used by the construction industries, which is known to be a heavy contributor of carbon dioxide emissions and environmental damage. Adding of industrial wastes like demolished old concrete OF structures, silica fume (SF) fly ash (FA) as additional cementing materials (SCMs) could result in a substantial reduction of the overall Carbon dioxide trace marks of the final concrete product. Use of these additional materials in construction industry especially in the making of concrete is highly challenging. Remarkable research efforts are needed to study about the engineering properties of concrete incorporating such industrial wastes. Present research is an effort to study the properties of concrete adding industrial wastes such as demolished concrete, FA and SF The improvement of properties of RCA concrete with the incorporation of two ureolytic-type bacteria, Bacillus subtilis and Bacillus sphaericus to improve the properties of RCA concrete. The experimental investigations are carried out by experts evaluate the improvement of the compressive strength, capillary water absorption and drying shrinkage of RCA concrete adding bacteria. Seven concrete mixes are manufactured using Portland slag cement (PSC) partially changed with SF ranging from 0 to 30%. The mix proportions were obtained as per Indian standard IS: 10262-2009 with 10% extra cement when SF is taken as per the above the construction practice by experts. Optimal dosages of SF for maximum values of compressive strength, tensile splitting strength and flexural strength at 28 days are determined. Keywords: Bacillus subtilis, Bacillus sphaericus, RCA, PSC, Silica Fume.

Self-Healing Biogeopolymers Using Biochar-Immobilized Spores of Pure- and Co-Cultures of Bacteria

A sustainable solution for crack maintenance in geopolymers is necessary if they are to be the future of modern green construction. This study aims to develop self-healing biogeopolymers that could potentially rival bioconcrete. First, a suitable healing agent was selected from Bacillus subtilis, Bacillus sphaericus, and Bacillus megaterium by directly adding their spores in the geopolymers and subsequently exposing them to a precipitation medium for 14 days. Scanning electron microscope with energy-dispersive X-ray (SEM-EDX) analysis revealed the formation of mineral phases for B. subtilis and B. sphaericus. Next, the effect of biochar-immobilization and co-culturing (B. sphaericus and B. thuringiensis) on the healing efficiencies of the geopolymers were tested and optimized by measuring their ultrasonic pulse velocities weekly over a 28-day healing period. The results show that using co-cultured bacteria significantly improved the observed efficiencies, while biochar-immobilization had a weak effect, but yielded an optimum response between 0.3–0.4 g/mL. The maximum crack width sealed was 0.65 mm. Through SEM-EDX and FTIR analyses, the precipitates in the cracks were identified to be mainly CaCO3. With that, there is potential in developing self-healing biogeopolymers using biochar-immobilized spores of bacterial cultures.