Correction: Metabolic constraints drive self-organization of specialized cell groups

AbstractHow phenotypically distinct states in isogenic cell populations appear and stably co-exist remains an unresolved question. We find that within a clonal yeast colony developing in low glucose, cells arrange into metabolically disparate cell groups. Using this system, we model and experimentally identify metabolic constraints sufficient to drive such assembly. Beginning in a gluconeogenic state, cells in a contrary state, exhibiting high pentose phosphate pathway activity, spontaneously appear and proliferate, in a spatially constrained manner. The gluconeogenic cells in the developing colony produce a resource, which we identify as trehalose. At threshold concentrations of trehalose, cells in the new metabolic state emerge and proliferate. A self-organized system establishes, where cells in this new state are sustained by trehalose consumption, which thereby restrains other cells in the trehalose producing, gluconeogenic state. Our work suggests simple physico-chemical principles that determine how isogenic cells spontaneously self-organize into structured assemblies in complimentary, specialized states.

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Decision letter: Metabolic constraints drive self-organization of specialized cell groups

10.7554/elife.46735.032 ◽

2019 ◽

Author(s):

Martin Ackermann

Keyword(s):

Self Organization ◽

Specialized Cell ◽

Cell Groups

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Author response: Metabolic constraints drive self-organization of specialized cell groups

10.7554/elife.46735.sa2 ◽

2019 ◽

Author(s):

Sriram Varahan ◽

Adhish Walvekar ◽

Vaibhhav Sinha ◽

Sandeep Krishna ◽

Sunil Laxman

Keyword(s):

Self Organization ◽

Author Response ◽

Specialized Cell ◽

Cell Groups

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Author response: Metabolic constraints drive self-organization of specialized cell groups

10.7554/elife.46735.033 ◽

2019 ◽

Author(s):

Sriram Varahan ◽

Adhish Walvekar ◽

Vaibhhav Sinha ◽

Sandeep Krishna ◽

Sunil Laxman

Keyword(s):

Self Organization ◽

Author Response ◽

Specialized Cell ◽

Cell Groups

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Self-Organization of Recognition Cell Groups in Nerve Fields

Systems and Computers in Japan ◽

10.1002/scj.4690200310 ◽

2007 ◽

Vol 20 (3) ◽

pp. 98-108 ◽

Cited By ~ 1

Author(s):

Nobuhiko Ikeda ◽

Toyoshi Torioka

Keyword(s):

Self Organization ◽

Cell Groups

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Metabolic constraints drive self-organization of specialized cell groups

eLife ◽

10.7554/elife.46735 ◽

2019 ◽

Vol 8 ◽

Cited By ~ 16

Author(s):

Sriram Varahan ◽

Adhish Walvekar ◽

Vaibhhav Sinha ◽

Sandeep Krishna ◽

Sunil Laxman

Keyword(s):

Self Assembly ◽

Pentose Phosphate ◽

Self Organization ◽

Cell Populations ◽

Pathway Activity ◽

Self Organized ◽

Physico Chemical ◽

Cell Groups ◽

Low Glucose ◽

Activity State

How phenotypically distinct states in isogenic cell populations appear and stably co-exist remains unresolved. We find that within a mature, clonal yeast colony developing in low glucose, cells arrange into metabolically disparate cell groups. Using this system, we model and experimentally identify metabolic constraints sufficient to drive such self-assembly. Beginning in a uniformly gluconeogenic state, cells exhibiting a contrary, high pentose phosphate pathway activity state, spontaneously appear and proliferate, in a spatially constrained manner. Gluconeogenic cells in the colony produce and provide a resource, which we identify as trehalose. Above threshold concentrations of external trehalose, cells switch to the new metabolic state and proliferate. A self-organized system establishes, where cells in this new state are sustained by trehalose consumption, which thereby restrains other cells in the trehalose producing, gluconeogenic state. Our work suggests simple physico-chemical principles that determine how isogenic cells spontaneously self-organize into structured assemblies in complimentary, specialized states.

Download Full-text