A framework for evaluating edited cell libraries created by massively parallel genome engineering

Genome engineering methodologies are transforming biological research and discovery. Approaches based on CRISPR technology have been broadly adopted and there is growing interest in the generation of massively parallel edited cell libraries. Comparing the libraries generated by these varying approaches is challenging and researchers lack a common framework for defining and assessing the characteristics of these libraries. Here we describe a framework for evaluating massively parallel libraries of edited genomes based on established methods for sampling complex populations. We define specific attributes and metrics that are informative for describing a complex cell library and provide examples for estimating these values. We also connect this analysis to generic phenotyping approaches, using either pooled (typically via a selection assay) or isolate (often referred to as screening) phenotyping approaches. We approach this from the context of creating massively parallel, precisely edited libraries with one edit per cell, though the approach holds for other types of modifications, including libraries containing multiple edits per cell (combinatorial editing). This framework is a critical component for evaluating and comparing new technologies as well as understanding how a massively parallel edited cell library will perform in a given phenotyping approach.

Parallelized Simulation of a Finite Element Method in Many Integrated Core Architecture

We investigate a domain decomposition method (DDM) of finite element method (FEM) using Intel's many integrated core (MIC) architecture in order to determine the most effective MIC usage. For this, recently introduced high-scalable parallel method of DDM is first introduced with a detailed procedure. Then, the Intel's Xeon Phi MIC architecture is presented to understand how to apply the parallel algorithm into a multicore architecture. The parallel simulation using the Xeon Phi MIC has an advantage that traditional parallel libraries such as the message passing interface (MPI) and the open multiprocessing (OpenMP) can be used without any additional libraries. We demonstrate the DDM using popular libraries for solving linear algebra such as the linear algebra package (LAPACK) or the basic linear algebra subprograms (BLAS). Moreover, both MPI and OpenMP are used for parallel resolutions of the DDM. Finally, numerical parallel efficiencies are validated by a two-dimensional numerical example.