Color and alcohol removal for the simultaneous detection of amino acids and sugars in wine by two-dimensional ion chromatography

2017 ◽  
Vol 1063 ◽  
pp. 36-41
Author(s):  
Yun Fa ◽  
Yinghui Liu ◽  
Aihua Xu ◽  
Yuexue Yu ◽  
Fangfang Li ◽  
...  
Keyword(s):  
Amino Acids ◽  
Ion Chromatography ◽  
Simultaneous Detection ◽  
Two Dimensional

Simultaneous detection of anions and cations in mineral water by two dimensional ion chromatography

2018 ◽  
Vol 1554 ◽  
pp. 123-127 ◽  
Author(s):  
Yun Fa ◽  
Yuexue Yu ◽  
Fangfang Li ◽  
Fanglin Du ◽  
Xiangfeng Liang ◽  
...  
Keyword(s):  
Ion Chromatography ◽  
Mineral Water ◽  
Simultaneous Detection ◽  
Two Dimensional ◽  
Anions And Cations

Simultaneous detection of anions and carbohydrates in cyanobacteria by two-dimensional ion chromatography

2020 ◽  
Author(s):  
Lijun Jiang ◽  
Yan Bao ◽  
Liang Guo ◽  
He Cui ◽  
Qi Wang ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Ion Chromatography ◽  
Solid Phase ◽  
Simultaneous Detection ◽  
Two Dimensional ◽  
Chromatography Method ◽  
Research Fields ◽  
Determination Of Anions ◽  
Valve Switching

Abstract Background The simultaneous analysis of several anions and carbohydrates by one-dimensional chromatography with a single detector is often complicated by the presence of overlapping peaks. To overcome this problem, analytes are usually analysed separately making analysis long and tedious. Objective A method combining two-dimensional ion chromatography (2 D-IC) and valve switching was developed for the simultaneous determination of anions (F−, Cl−, NO2−, SO42−, NO3−, and PO43−) and carbohydrates (glycerinum, glucosyl glycerol, trehalose, mannose, glucose, galactose, fructose, ribose, and sucrose) in cyanobacteria. Methods Interfering color compounds were removed by first passing the sample through graphitized carbom solid phase extraction (SPE) cartridges. Samples were applied to an AS11-HC column, which was used to separate the anions followed by quantification using a conductance detector. Carbohydrates eluted from the AS11-HC column were trapped and separated on a MA1 column and simultaneously quantified using electrochemical detection in the second dimension with valve switching. Results The following parameters were established: LOD, 0.001–0.030 (mg/L); LOQ, 0.001–0.010 (mg/L); linearity (r), 0.9940; repeatability, 0.39%–3.02%; and spiked recovery, 90.1%–107%. Conclusion The proposed method is adequately linear, accurate, and repeatable. The 2 D-IC method provides fast, high resolution, and completely automated procedure for the simultaneous determination of anions and carbohydrates without co-elution compared to the one dimension ion chromatography method. This study provides application perspectives for use in biotechnology and other research fields. Highlights An accurate and effective 2 D-IC method was developed for determining anions and carbohydrates in cyanobacteria. The method includes pre-treating samples with graphitized carbon SPE cartridges.

New Methods for Analyzing Water Pollutants

1982 ◽  
Vol 14 (4-5) ◽  
pp. 59-71 ◽  
Author(s):  
L H Keith ◽  
R C Hall ◽  
R C Hanisch ◽  
R G Landolt ◽  
J E Henderson
Keyword(s):  
Amino Acids ◽  
Gas Chromatography ◽  
Two Dimensional ◽  
Gas Chromatograph ◽  
New Class ◽  
New Methods ◽  
System A ◽  
Drinking Waters ◽  
Computer Controlled ◽  
Nitrogen Containing Compounds

Two new methods have been developed to analyze for organic pollutants in water. The first, two-dimensional gas chromatography, using post detector peak recycling (PDPR), involves the use of a computer-controlled gas Chromatograph to selectively trap compounds of interest and rechromatograph them on a second column, recycling them through the same detector again. The second employs a new detector system, a thermally modulated electron capture detector (TMECD). Both methods were used to demonstrate their utility by applying them to the analysis of a new class of potentially ubiquitous anthropoaqueous pollutants in drinking waters- -haloacetonitriles. These newly identified compounds are produced from certain amino acids and other nitrogen-containing compounds reacting with chlorine during the disinfection stage of treatment.

PAPER CHROMATOGRAPHY OF COMPLEX BIOLOGICAL MATERIALS BY SOLVENT REDEVELOPMENT WITHOUT PRIOR REMOVAL OF SALTS OR PROTEINS

1960 ◽  
Vol 38 (10) ◽  
pp. 1137-1147 ◽  
Author(s):  
Arthur E. Pasieka
Keyword(s):  
Amino Acids ◽  
Paper Chromatography ◽  
Filter Paper ◽  
Complex Mixtures ◽  
Two Dimensional ◽  
One Dimensional ◽  
Equivalent Time ◽  
Biological Compounds ◽  
High Concentrations ◽  
Subsequent Staining

A solvent redeveloping technique has been devised by which amino acids, peptides, and sugars can be separated from complex mixtures in the presence of high concentrations of salts and proteins. The separations are effected by two to four successive 18-hour solvent developments with drying between each 18-hour period before subsequent staining of the chromatograms. Better separations and resolutions are obtained by such successive 18-hour solvent developments than by one continuous solvent development for an equivalent time. The effect of these redevelopments on the separations and resolutions of biological compounds is illustrated at various stages by photographs of one- and two-dimensional chromatograms. The redevelopment technique requires filter paper sheets up to 4 ft in length for one-dimensional analytical and preparative types of chromatograms.

Role of N- and C-Terminal Amino Acids in Two-Dimensional Streptavidin Crystal Formation

Langmuir ◽  
2000 ◽  
Vol 16 (11) ◽  
pp. 5199-5204 ◽  
Author(s):  
Szu-Wen Wang ◽  
Channing Robertson ◽  
Alice Gast ◽  
Sandy Koppenol ◽  
Todd Edwards ◽  
...  
Keyword(s):  
Amino Acids ◽  
Crystal Formation ◽  
Two Dimensional ◽  
Terminal Amino
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