Potential Application of Indigenous Microorganism Derived Biosorbent in the Efficient Removal of Congo Red from Aqueous Solution

IMO compost was obtained using Indigenous Microorganisms (IMO) as inoculant through anaerobic fermentation process from kitchen waste biomass. The Physico-chemical analysis of the solid was obtained by taking pH, MC, OM, OC, N, K and P content. The biosorbent material was further characterized by SEM, IR and BET analytical techniques. Various physico-chemical parametric studies viz. effect of pH, initial dye concentration and background electrolyte concentrations are performed in the removal of Congo red (CR) dye using the IMO compost. The lower pH condition pH < 6.0 favoured the removal of Congo red and the percent removal was decreased with increase in the CR concentration. The removal of Congo red followed the pseudo-second order rate kinetics and the equilibrium state sorption data was reasonably fitted well to the Freundlich adsorption isotherm. In addition, the minimal effect of background electrolyte concentrations (0.0001 to 0.1 mol/L NaNO3) in the removal of CR inferred a specific sorption of Congo red forming ‘inner sphere’ complexes at the surface of the biosorbent.

Emerging trends for Harnessing plant metabolome and microbiome for sustainable food Production

Food production is obligatory to ensure efficient plant yield and accomplishments in a fast-growing global population predicted to exceed 9 trillion people in the future. In this regard, advancement in harnessing plant core microbiome using various 'omics' will be fruitful towards sustainable goals. Metabolomics, as we know, is a robust method and is very useful for evaluating the property of plant species before their genomes are completely sequenced. Metabolomics and other technologies facilitate us to unravel main agricultural output challenges such as regulating the health of soil microbial communities. Plantmicrobe interactions are an integral aspect of this planet. This review will develop a complete and elaborated panoramic view for bare acquaintances of interaction between plants and microbes required for developing different approaches in metagenomics, meta-transcriptomics, and metabolomics to increase general cultivable performance. In addition, the study will focus on microbiome development in earlier plant production and establish informatics pipelines for core microbiome design to optimize plant and indigenous microorganism interactions. This study will also concentrate on inter / intra interactions, such as quorum sensing activity, signal molecules like phytohormones, bacteriocins, etc. The bio-informatics genome mining for biosynthesis gene clusters leading to the identification of novel bioactive compounds and new metabolomics advances will also be established. A comprehensive model will be framed to assess the advancements in exploiting the microbiome and metabolome analysis to secure food production for a sustainable future.

Density and nitrifying potential of indigenous bacterial community in mangrove and seagrass in the north of Vietnam

Density and nitrification potential of indigenous microorganism in mangroves (Tien Yen - Quang Ninh and Bang La - Hai Phong) and seagrass (Ha Coi, Dam Ha - Quang Ninh and Tam Giang - Thua Thien Hue) in the north of Vietnam were evaluated through 4 sampling times in the dry and rainy seasons in the years of 2017-2019. The analytical results showed that the average density of nitrifying bacteria was 4.6 ± 1.8 × 102 MPN/ml, in which the density in mangroves tended to be higher than that in the seagrass beds (P < 0.05) in both the rainy and dry seasons. The average nitrifying rate was 2.7 ± 0.6 µgN/g wet soil/hour, in which the rate in mangroves tended to be higher than that in seagrass beds in the rainy season (P < 0.05). Nitrifying density and rate are not only correlated with substrate concentration but also with other environmental factors such as P-PO4, BOD5, total phosphate in sediment and total bacterial density.

Microbial diversity of Indigenous microorganism communities from different agri-ecosystems in Soc Trang province, Vietnam

Indigenous microorganism (IMO) has great potential for agricultural uses since they have high ability in biodegradation, nitrogen fixation, phosphate solubilization, plant growth hormone production as well as bio-control. This study aimed to determine the presence of some different major groups of microbes in IMO from different agri-ecosystem habitats like bacteria, fungi, actinomyces, Salmonella, Shigella, E. coli, and Coliform. The presence of bacteria, actinomyces, and fungi of IMO samples was identified by 27F/1492R, 243F/1378R and ITS1F/ITS4R primers, respectively. Numbers of bacteria, fungi, and actinomyces were determined by the plate counting method on TSA, PDA and Starch media, respectively. The numbers of Salmonella sp. and Shigella sp. were determined by the plate counting method on selective Salmonella and Shigella agar (SS agar) after incubation for 48 hours at 37oC while the density of Coliforms sp. and E.coli were counted by the Most Probable Number method (MPN). The results of the study showed that 3 major groups of microbes including bacteria, fungi, and actinomyces in 14 collected IMO samples were detected genetically. Moreover, bacterial numbers were dominated and ranged from 106 to 109 cfu/g IMO samples while the density of fungi and actinomyces were lower and varied from 105 to 107 cfu/g IMO sample. Interestingly, all surveyed IMO samples did not contain any human disease pathogens such as Salmonella, Shigella, Coliforms and E. coli. These results imply that collected IMO contains a high diversity of major groups of microbes and can be used as safe bio-stimulants for clean vegetable production.

The response of laying hens fed fermented pineapple peel waste by indigenous microorganism from bamboo sprout

ABSTRACT The bromelain enzyme content in pineapple peel waste predicted to promote digestive tract health and a positive effect on egg quality. This research aimed to evaluate the use of fermented pineapple peel waste in laying hens diet on egg quality. A total of laying hens 200 birds with Isa Brown strain, egg production of 70%, The average egg weight and body weight were 58.58 g/egg, and 1.62 g/bird, respectively. A completely randomized design was used, 5 different levels of fermented pineapple peel waste as treatment (0, 5, 10, 15, and 20%), and all treatments were repeated 4 times. Egg shell thickness, egg shell strength, haugh unit, egg yolk fat, and egg yolk color were measured. The use of fermented pineapple peel waste in the diet of laying hens up to 20% highly significant (P<0.01) on egg yolk color, and did not negative effect (P>0.05) on egg shell thickness, egg shell strength, haugh unit, and egg yolk fat. Fermented pineapple peel waste can be used as much as 20% in laying hens diet without disturbing egg quality and improve egg yolk color.

Effect of duration of time and dosage on fermentation of cacao pod by using Indigenous Microorganism YL (MOIYL)

The main problem with cocoa pod is the high crude fiber content and lowcrude protein. A treatment needed to change the nutritional content, one of which isfermentation. In this study, fermentation used Indigenous microorganisms which wereYL (MOIYL). This research lasted for 3 months which was carried out from July toSeptember 2018 at the Laboratory of Animal Production and the Laboratory of AnimalNutrition Sciences at the Faculty of Agriculture, University of North Sumatra. Theresearch design used was Factorial 4 x 4 Completely Randomized Design (CRD), with 3replications where factor 1 was the various levels of inoculum of Indigenous YL(MOIYL) and factor 2 was the length of fermentation (incubation) with measuredparameters of crude protein, crude fiber, crude fat, and ash content using proximateanalysis.The results showed that cocoa pod fermentation using Indigenous YL (MOIYL)microorganisms could improve the quality of nutrient content including the highestwater content with a dose of 3% with a 7 day fermentation of 11.75% and the lowestwithout Moiyl with 21 days fermentation time of 10.02%. highest crude protein with 5%dose with 7 days fermentation of 11.89% and the lowest without Moiyl with 28 daysfermentation of 7.18%, highest crude fat at 5% with 7 days fermentation at 1.33% andthe lowest without Moiyl with 28 days fermentation time is 1.22%, while the highestcrude fiber is 5% with 7 days fermentation of 21.3% and the lowest is 5% with 28 daysfermentation of 23.93%, the highest dry matter is 3% with 7 days fermentation timeequal to 88.24% and the lowest without Moiyl with 7 days long 89.95%, highest ashcontent with d osis 1% with a 7 day fermentation time of 9.52% and the lowest ashcontent without Moiyl with a 7 day fermentation time of 11.17%. The results of thisstudy concluded that cocoa pod fermentation using 5% Indigenous YL (MOIYL)microorganisms and 7 days fermentation time could increase water content, crudeprotein, and crude fat, while crude fiber, dry matter, and ash content decreased.

The Effect of Biofertilizer and Inorganic Fertilizer Toward The Nutrient Uptake in Maize Plant (Zea mays l.)

The productivity of land and plants have decreased slowly, due to the use of inorganic fertilizers continuously. Efforts to improve productivity are reducing inorganic fertilizers and returning organic matter to the soil. The aim of this research are (1) to identify the chemical characteristics of biofertilizer with an indigenous microorganism as bioactivators and (2) to study the effect of biofertilizer on nutrient uptake of maize plant. The research was conducted at Laboratory, Greenhouse, and Experimental Field of Politeknik Pertanian Negeri Payakumbuh, West Sumatra Indonesia. The first step experiment in the Laboratory was produced indigenous microorganism from banana humps. The second step of making bio-fertilizers in Greenhouse used a Completely Randomized Design with four treatments of indigenous microorganism (IMO) level 0%, 10%, 20%, 30% in biofertilizer with five replications. The third step of biofertilizer application on the Experimental Field used Randomized Block Design Factorial arranged with two factors and three replications, the first factor IMO level in biofertilizer (M) 0%, 10%, 20%, 30% and the second factor was the dosage of inorganic fertilizer (P) 0%, 50%, 100% of the recommended dosage, 12 combinations of treatments were obtained. Results showed that microbe consortium (Enterobacter cloacae, Bacillus subtilis, Pseudomonas fluorescens, Aspergillus niger, and Trichoderma asperellum) in biofertilizers with different levels would affect the chemical characteristics of biofertilizer. Biofertilizers influences nutrient uptake of P and K maize plant, while inorganic fertilizer influences nutrient uptake of N and P maize plant.