Fluorescent antibody studies in pemphigus vulgaris

1966 ◽  
Vol 93 (5) ◽  
pp. 562-569 ◽  
Author(s):  
E. T. Wright
Author(s):  
Kenjiro Yasuda

Localization of amylase,chymotrypsinogen and trypsinogen in pancreas was demonstrated by Yasuda and Coons (1966), by using fluorescent antibody method. These enzymes were naturally found in the zymogen granules. Among them, amylase showed a diffuse localization around the nucleus, in addition to the zymogen granules. Using ferritin antibody method, scattered ferritin granules were also found around the Golgi area (Yasuda et al.,1967). The recent advance in the tissue preparation enables the antigen to be localized in the ultrathin frozen sections, by applying the labeled antibodies onto the sections instead of staining the tissue en bloc.The present study deals with the comparison of the localization of amylase and lipase demonstrated by applying the bismuth-labeled, peroxidase-labeled and ferritin-labeled antibody methods on the ultrathin frozen sections of pancreas, and on the blocks of the same tissue.


2001 ◽  
Vol 144 (2) ◽  
pp. 421-422 ◽  
Author(s):  
K. Ogata ◽  
H. Nakajima ◽  
M. Ikeda ◽  
Y. Yamamoto ◽  
M. Amagai ◽  
...  

1973 ◽  
Vol 107 (5) ◽  
pp. 751-751 ◽  
Author(s):  
A. Baumal
Keyword(s):  

1969 ◽  
Vol 99 (2) ◽  
pp. 176-179 ◽  
Author(s):  
R. E. Jordon
Keyword(s):  

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