Investigation and circumvention of transfection inhibition by ferric ammonium citrate in serum‐free media for Chinese hamster ovary cells

2019 ◽  
Vol 36 (3) ◽  
Author(s):  
Berta Capella Roca ◽  
Nga T. Lao ◽  
Martin Clynes ◽  
Padraig Doolan
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ammonium Citrate ◽  
Ferric Ammonium Citrate ◽  
Ovary Cells ◽  
Serum Free ◽  
Ferric Ammonium ◽  
Free Media

Effects of phospholipids on growth of Chinese hamster ovary cells in serum-free media

1999 ◽  
Vol 88 (3) ◽  
pp. 306-309 ◽  
Author(s):  
Kentaro Sakai ◽  
Toshio Matsunaga ◽  
Hideki Yamaji ◽  
Hideki Fukuda
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells ◽  
Serum Free ◽  
Free Media

scholarly journals Polyamine starvation causes accumulation of cadaverine and its derivatives in a polyamine-dependent strain of Chinese-hamster ovary cells

1983 ◽  
Vol 210 (3) ◽  
pp. 945-948 ◽  
Author(s):  
E Hölttä ◽  
P Pohjanpelto
Keyword(s):  
Cell Proliferation ◽  
Ornithine Decarboxylase ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Specific Inhibitor ◽  
Ovary Cells ◽  
Serum Free ◽  
Dependent Strain

Starvation of the polyamine-dependent Chinese-hamster ovary cells for ornithine or ornithine-derived polyamines in serum-free culture resulted in the formation of cadaverine and its aminopropyl derivatives, N-(3-aminopropyl)cadaverine and NN'-bis(3-aminopropyl)cadaverine. The synthesis of these unusual amines was inhibited by treatment of the cells with DL-2-difluoromethylornithine, a specific inhibitor of ornithine decarboxylase (EC 4.1.1.17). In the absence of ornithine (the normal substrate), ornithine decarboxylase thus appeared to catalyse the decarboxylation of lysine to cadaverine. Cell proliferation was markedly inhibited by ornithine deprivation of the cells, and further depressed by exposure of the cultures to difluoromethylornithine.

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